Rabbit Polyclonal Trehalase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human TREH aa 50-150 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TREH aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O43280
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Intestinal trehalase is probably involved in the hydrolysis of ingested trehalose.
Alternative names
TREA, TREH, Trehalase
Recommended products
Rabbit Polyclonal Trehalase antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human TREH aa 50-150 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TREH aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link O43280
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Trehalase also known as TREH is an enzyme responsible for the hydrolysis of trehalose into two glucose molecules. It has a molecular mass of approximately 65 kDa. This enzyme is mainly expressed in the small intestine brush border membrane and kidney. The presence of trehalase in these regions of the body suggests it plays an important role in carbohydrate digestion.
- Biological function summary
Trehalase facilitates the conversion of trehalose a non-reducing disaccharide which serves as an important energy source for various organisms. By breaking down trehalose the enzyme contributes to glucose homeostasis within the body. Trehalase does not form part of any known multi-protein complexes functioning as an independent enzyme in cellular metabolism.
- Pathways
Trehalase participates in the carbohydrate digestion and absorption pathway. This pathway involves converting dietary carbohydrates into simple sugars that the body can utilize for energy. Trehalase operates in conjunction with other enzymes like lactase and sucrase-isomaltase that also contribute to carbohydrate digestion within the gastrointestinal tract.
- Associated diseases and disorders
Trehalase deficiency can lead to digestive issues notably trehalose intolerance where affected individuals experience symptoms after consuming foods containing trehalose. Additionally trehalase activity has connections to diabetes as it directly influences glucose levels and metabolism. Adjustments in trehalase activity may affect the concentration of glucose in the bloodstream impacting individuals with diabetes by potentially altering their glucose homeostasis.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Trehalase antibody (ab217293)
All lanes: Western blot - Anti-Trehalase antibody (ab217293) at 1/500 dilution
All lanes: mouse stomach lysate
SecondaryAll lanes: HRP conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution
Predicted band size: 67 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trehalase antibody (ab217293)
Immunohistochemical analysis of formalin-fixed paraffin-embedded sections of human kidney tissue labeling Trehalase with ab217293 at a 1/200 dilution, followed by conjugation to a secondary antibody and DAB staining.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com