AB225861

Anti-TREM1 antibody [EPR22060-229]

RabMAb
Recombinant
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(9 Publications)

Anti-TREM1 antibody [EPR22060-229] (ab225861) is a rabbit monoclonal antibody suitable for IHC-P and reacts with Human samples. Cited in 9 publications.

Enhanced IHC validation data - Download the data
- detailed IHC expression profiling in normal and clinically relevant cancer FFPE TMAs- colon, lung, head and neck
- TREM1/CD14 duplex staining in relevant disease samples - COVID-19, glioblastoma, colitis
- singleplex/duplex protocols for BOND RX™ and Ventana DISCOVERY ULTRA
Cited in 9 publications
Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD354, Triggering receptor expressed on myeloid cells 1, TREM-1, Triggering receptor expressed on monocytes 1, TREM1

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

IHC image of TREM1 staining in a section of formalin-fixed paraffin-embedded normal human tonsil*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated overnight at +4°C with ab225861 at 1 µg/ml and ab783, Mouse monoclonal [PG-M1] to CD68, at 5 µg/ml and then detected with an Alexa Fluor® 594 goat anti-rabbit secondary antibody (ab150080) at a 1/1000 dilution (shown in red) and Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

IHC image of TREM1 staining in a section of formalin-fixed paraffin-embedded normal human lung*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was then incubated overnight at +4°C with ab225861 at 1 µg/ml and ab783, Mouse monoclonal [PG-M1] to CD68, at 5 µg/ml and then detected with an Alexa Fluor® 594 goat anti-rabbit secondary antibody (ab150080) at a 1/1000 dilution (shown in red) and Alexa Fluor® 488 goat anti-mouse secondary antibody (ab150117) at a 1/1000 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue). The section was then mounted using Dako Fluorescence Mounting Medium®. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). For other IHC staining systems (automated and non-automated), customers should optimize variable parameters such as antigen retrieval conditions, antibody concentrations and incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemical analysis of paraffin-embedded human colon tissue labeling TREM1 with ab225861 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on interstitial cells of human colon (PMID : 17853946; PMID : 20933010) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling TREM1 with ab225861 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on interstitial cells and lymphocytes in human stomach (PMID : 17853946; PMID : 20933010) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemical analysis of paraffin-embedded human lung tissue labeling TREM1 with ab225861 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on alveolar macrophages in human lung (PMID : 12792857; PMID : 20933010) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Carrier free

Anti-TREM1 antibody [EPR22060-229] - BSA and Azide free
Unconjugated

Anti-TREM1 antibody [EPR22060-229] - Mouse IgG2a (Chimeric)
Carrier free

Anti-TREM1 antibody [EPR22060-229] - Mouse IgG2a (Chimeric) - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22060-229

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Triggering receptor expressed on myeloid cells 1 (TREM1) is a transmembrane receptor involved in amplifying inflammatory responses. TREM1 is known alternatively as CD354. This protein has a molecular mass of approximately 30 kDa and is expressed on the surface of neutrophils monocytes and macrophages. The receptor participates in innate immune responses and acts as an amplifier for inflammatory signals. It is often found in tissues that are engaged in inflammatory processes including lungs and intestines.

Biological function summary

TREM1 significantly enhances the immune response by activating myeloid cells. It does not form a standalone unit; instead it works with the adapter protein DNAX-activating protein of 12 kDa (DAP12) which pairs with TREM1 to transduce signals that promote cytokine and chemokine release. Through this interaction the receptor plays a role in the defense against bacterial infection boosting the body's immune response during pathogen assault. This function positions TREM1 as an important player in the regulation of acute inflammation.

Pathways

TREM1 engages significantly in the Toll-like receptor (TLR) signaling pathway and NF-kB pathway. In these pathways TREM1 interacts with various proteins that include TLRs and DAP12 intensifying inflammatory responses. The integration of TREM1 within these pathways suggests that it acts as an important modulator of inflammation capable of enhancing TLR-mediated signals for a stronger immune response.

TREM1 connections extend to sepsis and inflammatory bowel disease (IBD). In the context of sepsis TREM1 expression correlates with increased inflammation and poor prognosis where it interacts with proteins involved in inflammatory pathways such as TNF-α. Concerning IBD TREM1's role in promoting inflammation links it to the pathogenesis of this disease where it could influence the severity and progression of IBD-related inflammation. Given these relationships therapeutic strategies targeting TREM1 could hold potential in modulating these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting
Download enhancedValidationDataWebsite|en

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Target data

Isoform 1. Cell surface receptor that plays important roles in innate and adaptive immunity by amplifying inflammatory responses (PubMed : 10799849, PubMed : 21393102). Upon activation by various ligands such as PGLYRP1, HMGB1 or HSP70, multimerizes and forms a complex with transmembrane adapter TYROBP/DAP12 (PubMed : 17568691, PubMed : 25595774, PubMed : 29568119). In turn, initiates a SYK-mediated cascade of tyrosine phosphorylation, activating multiple downstream mediators such as BTK, MAPK1, MAPK3 or phospholipase C-gamma (PubMed : 14656437, PubMed : 21659545). This cascade promotes the neutrophil- and macrophage-mediated release of pro-inflammatory cytokines and/or chemokines, as well as their migration and thereby amplifies inflammatory responses that are triggered by bacterial and fungal infections (PubMed : 17098818, PubMed : 17568691). By also promoting the amplification of inflammatory signals that are initially triggered by Toll-like receptor (TLR) and NOD-like receptor engagement, plays a major role in the pathophysiology of acute and chronic inflammatory diseases of different etiologies including septic shock and atherosclerosis (PubMed : 11323674, PubMed : 21393102).. Isoform 2. Acts as a decoy receptor, counterbalancing TREM1 pro-inflammatory activity through the neutralization of its ligand.

See full target information TREM1

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Discover oncology 16:1624 PubMed40855239

2025

DNA methylation regulates TREM1 expression to modulate immune responses and drive progression in colorectal neuroendocrine neoplasm as a potential therapeutic target.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Guo,Guiwen Zheng,Jia Li,Shuzhan Yao,Qiang Jia,Jian Tan,Zhaowei Meng

International journal of molecular sciences 26: PubMed40362275

2025

Identification and Exploration of Pyroptosis-Related Genes in Macrophage Cells Reveal Necrotizing Enterocolitis Heterogeneity Through Single-Cell and Bulk-Sequencing.

Applications

Unspecified application

Species

Unspecified reactive species

Peipei Zhang,Ying Li,Panpan Xu,Peicen Zou,Sihan Sheng,Ruiqi Xiao,Pu Xu,Ying Chen,Yue Du,Lishuang Ma,Yajuan Wang

Frontiers in immunology 15:1324010 PubMed38370418

2024

Molecular and immunological features of TREM1 and its emergence as a prognostic indicator in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhang,Xun Qu,Yangyang Xu

iScience 26:107585 PubMed37694144

2023

Multi-omic approach identifies hypoxic tumor-associated myeloid cells that drive immunobiology of high-risk pediatric ependymoma.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea M Griesinger,Kent Riemondy,Nithyashri Eswaran,Andrew M Donson,Nicholas Willard,Eric W Prince,Simon M L Paine,Georgia Bowes,John Rheaume,Rebecca J Chapman,Judith Ramage,Andrew Jackson,Richard G Grundy,Nicholas K Foreman,Timothy A Ritzmann

OncoTargets and therapy 16:331-345 PubMed37274309

2023

High Expression of Triggering Receptor Expressed on Myeloid Cells 1 Predicts Poor Prognosis in Glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ke Ma,Qianqian Guo,Xianwei Zhang,Yanxin Li

EMBO reports 23:e54558 PubMed35856334

2022

FOXM1 network in association with TREM1 suppression regulates NET formation in diabetic foot ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew P Sawaya,Rivka C Stone,Spencer Mehdizadeh,Irena Pastar,Stephen Worrell,Nathan C Balukoff,Mariana J Kaplan,Marjana Tomic-Canic,Maria I Morasso

Theranostics 11:3624-3641 PubMed33664852

2021

Genome-wide CRISPR/Cas9 knockout screening uncovers a novel inflammatory pathway critical for resistance to arginine-deprivation therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng-Ying Chu,Yi-Ching Lee,Cheng-Han Hsieh,Chi-Tai Yeh,Tsu-Yi Chao,Po-Hung Chen,I-Hsuan Lin,Tsung-Han Hsieh,Jing-Wen Shih,Chia-Hsiung Cheng,Che-Chang Chang,Ping-Sheng Lin,Yuan-Li Huang,Tsung-Ming Chen,Yun Yen,David K Ann,Hsing-Jien Kung

Redox biology 24:101201 PubMed31039480

2019

Mitochondrial oxidative stress in the retinal pigment epithelium (RPE) led to metabolic dysfunction in both the RPE and retinal photoreceptors.

Applications

Unspecified application

Species

Unspecified reactive species

Emily E Brown,Alexander J DeWeerd,Cristhian J Ildefonso,Alfred S Lewin,John D Ash

EBioMedicine 42:363-374 PubMed30885723

2019

Development and validation of a TP53-associated immune prognostic model for hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Junyu Long,Anqiang Wang,Yi Bai,Jianzhen Lin,Xu Yang,Dongxu Wang,Xiaobo Yang,Yan Jiang,Haitao Zhao

View all publications

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enhancedValidationDataWebsite

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TREM1 antibody [EPR22060-229]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM1 antibody [EPR22060-229] (AB225861)

Related conjugates and formulations (3)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (9)

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