Goat Polyclonal TREM2 antibody. Suitable for ICC and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Mouse Trem2 aa 150-200.
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- Nature metabolism 5:129-1462023TREM2 resident macrophages protect the septic heart by maintaining cardiomyocyte homeostasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai Zhang,Yang Wang,Shiyu Chen,Jiali Mao,Yue Jin,Hui Ye,Yan Zhang,Xiwang Liu,Chenchen Gong,Xuejun Cheng,Xiaoli Huang,Andreas Hoeft,Qixing Chen,Xuekun Li,Xiangming FangPubMed 36635449
- Frontiers in immunology 13:9380752022Single-cell RNA-Seq reveals changes in immune landscape in post-traumatic osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAimy Sebastian,Nicholas R Hum,Jillian L McCool,Stephen P Wilson,Deepa K Murugesh,Kelly A Martin,Naiomy Deliz Rios-Arce,Beheshta Amiri,Blaine A Christiansen,Gabriela G LootsPubMed 35967299
- Brain : a journal of neurology 143:336-3582019Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaya Koronyo-Hamaoui,Julia Sheyn,Eric Y Hayden,Songlin Li,Dieu-Trang Fuchs,Giovanna C Regis,Dahabada H J Lopes,Keith L Black,Kenneth E Bernstein,David B Teplow,Sebastien Fuchs,Yosef Koronyo,Altan RentsendorjPubMed 31794021
- FASEB journal : official publication of the Federa 33:12164-121742019TREM2 deficiency aggravates α-synuclein-induced neurodegeneration and neuroinflammation in Parkinson's disease models.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Guo et. al.PubMed 31370707
- Frontiers in immunology 10:12412019The Intra-nuclear SphK2-S1P Axis Facilitates M1-to-M2 Shift of Microglia via Suppressing HDAC1-Mediated KLF4 Deacetylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Ji et. al.PubMed 31214192
- Cell stem cell 24:654-669.e62019A Subset of TREM2 Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEtienne C E Wang et. al.PubMed 30930146
- Journal of neuroinflammation 14:592017The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Jendresen et. al.PubMed 28320424
- Journal of Alzheimer's disease : JAD 55:199-2172016Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model.Applications:IHC, WBReactive species:MouseAnimesh Alexander Raha,James W Henderson,Simon R W Stott,Romina Vuono,Simona Foscarin,Robert P Friedland,Shahid H Zaman,Ruma Raha-ChowdhuryPubMed 27662313
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Trem2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q99NH8
Reactivity data
Select an application| ICC | |
|---|---|
| Mouse | Tested |
| Rat | Predicted |
ICC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 10 mg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
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Target data
Function
Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed:27477018, PubMed:29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed:28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed:15728241, PubMed:25631124, PubMed:28592261, PubMed:29752066). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed:28483841, PubMed:29663649, PubMed:29859094, PubMed:30232263). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed:29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed:28592261). Plays a role in microglial MTOR activation and metabolism (PubMed:28802038). Regulates age-related changes in microglial numbers (PubMed:25631124, PubMed:29752066, PubMed:30548312). Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:16715077). Involved in the positive regulation of osteoclast differentiation (PubMed:16418779).
Alternative names
Trem2a, Trem2b, Trem2c, Trem2, Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2
Recommended products
Goat Polyclonal TREM2 antibody. Suitable for ICC and reacts with Mouse samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Mouse Trem2 aa 150-200.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Trem2 aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q99NH8
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TREM2 also known as Triggering Receptor Expressed on Myeloid Cells 2 functions as a receptor with an important role in the immune system. This protein weighing about 230-290 kDa mostly expresses in myeloid cells which include macrophages monocytes and microglia. It serves as an important player in signaling for the activation of these cells. Researchers often study TREM2's functions through various species including cynomolgus monkeys to understand its implications better.
- Biological function summary
TREM2 significantly influences immune responses by participating in cellular clearance functions such as phagocytosis. It forms a receptor complex with DAP12 which transduces signals leading to the activation of immune responses. TREM2 aids in regulating inflammatory responses and ensures the maintenance of tissue homeostasis in healthy and diseased states. Its role extends to the control of lipid metabolism particularly in the central nervous system.
- Pathways
Scientific studies link TREM2 to the immune-inflammatory pathway and the neurodegenerative pathway. Within these pathways TREM2 interacts notably with proteins such as DAP12 and SYK. Through these interactions TREM2 contributes to signaling cascades that modulate inflammation and neurodegenerative processes within the brain supporting cellular communication and survival.
- Associated diseases and disorders
TREM2's functionality connects significantly with Alzheimer's disease and various inflammatory conditions. In Alzheimer's disease mutations in TREM2 alter its normal activity potentially increasing neuroinflammation and advancing disease progression. Additionally collaborations between TREM2 and ApoE proteins further highlight its involvement in lipid regulation within neurodegenerative conditions. Understanding TREM2’s role can pave the way for targeted therapeutic approaches in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry - Anti-TREM2 antibody (ab95470)
Immunocytochemistry/ Immunofluorescence analysis of paraformaldehyde fixed mouse 3T3-L1 cells labeling TREM2 with ab95470 at 10 μg/mL (incubation time, 1 hour). Cells were permeabilized with 0.15% Triton. Alexa Fluor 488 secondary antibody was used at 2μg/mL. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 μg/mL followed by Alexa Fluor 488 secondary antibody 2 μg/mL.
Immunocytochemistry - Anti-TREM2 antibody (ab95470)
Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation with ab95470 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
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