Anti-TREM2 antibody - C-terminal

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody - C-terminal (AB223684)

Paraffin-embedded human lung tissue stained for TREM2 using ab223684 at 2 μg/ml in immunohistochemical analysis, followed by AP-staining.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody - C-terminal (AB223684)

Immunofluorescence analysis of paraformaldehyde fixed U937 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1hr using ab223684 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• Flow Cyt

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Flow Cytometry - Anti-TREM2 antibody - C-terminal (AB223684)

Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr using ab223684 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody - C-terminal (AB223684)

Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr using ab223684 (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

• WB

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Western blot - Anti-TREM2 antibody - C-terminal (AB223684)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-TREM2 antibody - C-terminal (ab223684) at 0.3 µg/mL

Lane 1:

Human cerebellum lysate in RIPA buffer at 35 µg

Lane 2:

Human frontal cortex lysate in RIPA buffer at 35 µg

Lane 3:

Human hippocampus lysate in RIPA buffer at 35 µg

Predicted band size: 25 kDa

Observed band size: 24 kDa

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