Knockout Tested Rabbit Recombinant Monoclonal TREM2 antibody. Suitable for IHC-P, WB, IP, mIHC and reacts with Transfected cell line - Human, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
IHC-P | WB | IP | mIHC | Flow Cyt | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:10799849). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (By similarity). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (PubMed:29794134). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (By similarity). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (By similarity). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (By similarity). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (By similarity). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (By similarity). Plays a role in microglial MTOR activation and metabolism (By similarity). Regulates age-related changes in microglial numbers (PubMed:29752066). Triggers activation of the immune responses in macrophages and dendritic cells (PubMed:10799849). Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (By similarity). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:11602640). Involved in the positive regulation of osteoclast differentiation (PubMed:12925681).
Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2, TREM2
Knockout Tested Rabbit Recombinant Monoclonal TREM2 antibody. Suitable for IHC-P, WB, IP, mIHC and reacts with Transfected cell line - Human, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR26209-22
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
This supplementary information is collated from multiple sources and compiled automatically.
TREM2 also known as Triggering Receptor Expressed on Myeloid Cells 2 functions as a receptor with an important role in the immune system. This protein weighing about 230-290 kDa mostly expresses in myeloid cells which include macrophages monocytes and microglia. It serves as an important player in signaling for the activation of these cells. Researchers often study TREM2's functions through various species including cynomolgus monkeys to understand its implications better.
TREM2 significantly influences immune responses by participating in cellular clearance functions such as phagocytosis. It forms a receptor complex with DAP12 which transduces signals leading to the activation of immune responses. TREM2 aids in regulating inflammatory responses and ensures the maintenance of tissue homeostasis in healthy and diseased states. Its role extends to the control of lipid metabolism particularly in the central nervous system.
Scientific studies link TREM2 to the immune-inflammatory pathway and the neurodegenerative pathway. Within these pathways TREM2 interacts notably with proteins such as DAP12 and SYK. Through these interactions TREM2 contributes to signaling cascades that modulate inflammation and neurodegenerative processes within the brain supporting cellular communication and survival.
TREM2's functionality connects significantly with Alzheimer's disease and various inflammatory conditions. In Alzheimer's disease mutations in TREM2 alter its normal activity potentially increasing neuroinflammation and advancing disease progression. Additionally collaborations between TREM2 and ApoE proteins further highlight its involvement in lipid regulation within neurodegenerative conditions. Understanding TREM2’s role can pave the way for targeted therapeutic approaches in these disorders.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
TREM2 was immunoprecipitated from 0.35 mg THP-1 (human monocytic leukemia monocyte) whole cell lysate with ab318262 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab318262 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 2: ab318262 IP in THP-1 (human monocytic leukemia monocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab318262 in THP-1 whole cell lysate
All lanes: Immunoprecipitation - Anti-TREM2 antibody [EPR26209-22] (ab318262) at 1/30 dilution
All lanes: THP-1 (human monocytic leukemia monocyte) whole cell lysate
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 84s
In Western blot, ab318262 was shown to bind specifically to TREM2. Target of interest was observed at 30 kDa in wild-type THP-1 cell lysates (lane 1) with no signal observed at this size in TREM2 knockout cell line (lane 2) (lane 2, knockout cell line Human TREM2 knockout THP-1 cell line ab269489 / knockout cell lysate Human TREM2 knockout THP-1 cell lysate ab269652).
Negative control: SH-SY5Y, HL-60.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TREM2 antibody [EPR26209-22] (ab318262) at 1/1000 dilution
Lane 1: Wild-type THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg
Lane 2: TREM2 knockout THP-1 whole cell lysate at 20 µg
Lane 3: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 4: HL-60 (human acute promyelocytic leukemia promyeloblast) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 30 kDa, 36 kDa
Exposure time: 180s
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human astrocytoma tissue staining TREM2 with ab318262 at a 1:100 (5.29 ug/ml) dilution, Anti-TMEM119 antibody [EPR25865-89] ab306583 anti-TMEM119 used at 1:2000 (0.255 ug/ml) dilution and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 anti-GFAP used at a 1:1000 (1.325 ug/ml) dilution.
Panel A: merged staining of anti-TREM2 (green; Opal™520), anti-TMEM119 (magenta; Opal™690) and anti-GFAP (yellow; Opal™570) on human astrocytoma.
Panel B: anti-TREM2 staining microglia in human astrocytoma.
Panel C: anti-TMEM119 staining microglia in human astrocytoma.
Panel D: anti-GFAP staining astrocyte in human astrocytoma.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab318262, Anti-TMEM119 antibody [EPR25865-89] ab306583 and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human cerebrum tissue staining TREM2 with ab318262 at a 1:100 (5.29 ug/ml) dilution, Anti-TMEM119 antibody [EPR25865-89] ab306583 anti-TMEM119 used at 1:2000 (0.255 ug/ml) dilution and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 anti-GFAP used at a 1:1000 (1.325 ug/ml) dilution.
Panel A: merged staining of anti-TREM2 (green; Opal™520), anti-TMEM119 (magenta; Opal™690) and anti-GFAP (yellow; Opal™570) on human cerebrum.
Panel B: anti-TREM2 staining microglia in human cerebrum.
Panel C: anti-TMEM119 staining microglia in human cerebrum.
Panel D: anti-GFAP staining astrocytes in human cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).
The section was incubated in three rounds of staining: in the order of ab318262, Anti-TMEM119 antibody [EPR25865-89] ab306583 and Anti-GFAP antibody [EPR1034Y] - BSA and Azide free ab218309 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Nuclear counter stain with DAPI.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver (PMID: 12472885). The section was incubated with ab318262 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded (A) THP-1 (human monocytic leukemia monocyte) cell pellet (B) SH-SY5Y (human neuroblastoma epithelial cell) cell pellet (C) HL-60 (human acute promyelocytic leukemia promyeloblast) cell pellet tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) THP-1 cell pellet, negative staining on (B) SH-SY5Y cell pellet and (C) HL-60 cell pellet. The section was incubated with ab318262 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human Alzheimer's cerebrum tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human Alzheimer's cerebrum (PMID: 25186950; : 25186950; PMID: 28592261 ). The section was incubated with ab318262 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a TREM2 expression vector containing a Myc-His tag. (B) HEK-293T transfected with TREM1 expression vector containing a His tag. (C) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling TREM2 with ab318262 at 1/2000 (0.265 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on (A) HEK-293T (human epithelial cell line from embryonic kidney) transfected with a TREM2 expression vector containing a Myc-His tag, negative staining on (B) HEK-293T transfected with TREM1 expression vector containing a His tag and (C) HEK-293T transfected with empty vector containing a Myc-His tag. The section was incubated with ab318262 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human glioblastoma tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human glioblastoma. The section was incubated with ab318262 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemical analysis of paraffin-embedded Human astrocytoma tissue labeling TREM2 with ab318262 at 1/100 (5.29 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human astrocytoma. The section was incubated with ab318262 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
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