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Anti-TREM2 antibody [EPR26210-1] ab305103 is a rabbit monoclonal antibody that is used in TREM2 western blotting, IHC and immunofluorescence. Suitable for mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Validated on the Leica BOND™ RX automated IHC staining platform for IHC
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (AB305103), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (AB305103), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (AB305103), expandable thumbnail
  • Western blot - Anti-TREM2 antibody [EPR26210-1] (AB305103), expandable thumbnail
  • Western blot - Anti-TREM2 antibody [EPR26210-1] (AB305103), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFIHC-FrIPFlow Cyt (Intra)
Human
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Mouse
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Actual Band 35-50, 25

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Actual Band 35-50, 25

Species
Rat
Dilution info
-
Notes

Actual Band 35-50, 25

Species
Transfected cell line - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Transfected cell line - Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Not recommended
Not recommended

Species
Human, Rat, Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Rat, Mouse, Human, Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Transfected cell line - Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human, Rat, Transfected cell line - Mouse
Dilution info
-
Notes

-

Target data

Function

Forms a receptor signaling complex with TYROBP which mediates signaling and cell activation following ligand binding (PubMed:11241283). Acts as a receptor for amyloid-beta protein 42, a cleavage product of the amyloid-beta precursor protein APP, and mediates its uptake and degradation by microglia (PubMed:27477018, PubMed:29518356). Binding to amyloid-beta 42 mediates microglial activation, proliferation, migration, apoptosis and expression of pro-inflammatory cytokines, such as IL6R and CCL3, and the anti-inflammatory cytokine ARG1 (PubMed:27477018, PubMed:29518356). Acts as a receptor for lipoprotein particles such as LDL, VLDL, and HDL and for apolipoproteins such as APOA1, APOA2, APOB, APOE, APOE2, APOE3, APOE4, and CLU and enhances their uptake in microglia (PubMed:27477018). Binds phospholipids (preferably anionic lipids) such as phosphatidylserine, phosphatidylethanolamine, phosphatidylglycerol and sphingomyelin (By similarity). Regulates microglial proliferation by acting as an upstream regulator of the Wnt/beta-catenin signaling cascade (PubMed:28077724). Required for microglial phagocytosis of apoptotic neurons (PubMed:24990881). Also required for microglial activation and phagocytosis of myelin debris after neuronal injury and of neuronal synapses during synapse elimination in the developing brain (PubMed:15728241, PubMed:25631124, PubMed:28592261, PubMed:29752066). Regulates microglial chemotaxis and process outgrowth, and also the microglial response to oxidative stress and lipopolysaccharide (PubMed:28483841, PubMed:29663649, PubMed:29859094, PubMed:30232263). It suppresses PI3K and NF-kappa-B signaling in response to lipopolysaccharide; thus promoting phagocytosis, suppressing pro-inflammatory cytokine and nitric oxide production, inhibiting apoptosis and increasing expression of IL10 and TGFB (PubMed:29663649). During oxidative stress, it promotes anti-apoptotic NF-kappa-B signaling and ERK signaling (PubMed:28592261). Plays a role in microglial MTOR activation and metabolism (PubMed:28802038). Regulates age-related changes in microglial numbers (PubMed:25631124, PubMed:29752066, PubMed:30548312). Triggers activation of the immune responses in macrophages and dendritic cells. Mediates cytokine-induced formation of multinucleated giant cells which are formed by the fusion of macrophages (PubMed:18957693). In dendritic cells, receptor of SEMA6D with PLEXNA1 as coreceptor and mediates up-regulation of chemokine receptor CCR7 and dendritic cell maturation and survival (PubMed:16715077). Involved in the positive regulation of osteoclast differentiation (PubMed:16418779).

Alternative names

Trem2a, Trem2b, Trem2c, Trem2, Triggering receptor expressed on myeloid cells 2, TREM-2, Triggering receptor expressed on monocytes 2

Recommended products

Anti-TREM2 antibody [EPR26210-1] ab305103 is a rabbit monoclonal antibody that is used in TREM2 western blotting, IHC and immunofluorescence. Suitable for mouse samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Validated on the Leica BOND™ RX automated IHC staining platform for IHC
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR26210-1
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TREM2 also known as Triggering Receptor Expressed on Myeloid Cells 2 functions as a receptor with an important role in the immune system. This protein weighing about 230-290 kDa mostly expresses in myeloid cells which include macrophages monocytes and microglia. It serves as an important player in signaling for the activation of these cells. Researchers often study TREM2's functions through various species including cynomolgus monkeys to understand its implications better.

Biological function summary

TREM2 significantly influences immune responses by participating in cellular clearance functions such as phagocytosis. It forms a receptor complex with DAP12 which transduces signals leading to the activation of immune responses. TREM2 aids in regulating inflammatory responses and ensures the maintenance of tissue homeostasis in healthy and diseased states. Its role extends to the control of lipid metabolism particularly in the central nervous system.

Pathways

Scientific studies link TREM2 to the immune-inflammatory pathway and the neurodegenerative pathway. Within these pathways TREM2 interacts notably with proteins such as DAP12 and SYK. Through these interactions TREM2 contributes to signaling cascades that modulate inflammation and neurodegenerative processes within the brain supporting cellular communication and survival.

Associated diseases and disorders

TREM2's functionality connects significantly with Alzheimer's disease and various inflammatory conditions. In Alzheimer's disease mutations in TREM2 alter its normal activity potentially increasing neuroinflammation and advancing disease progression. Additionally collaborations between TREM2 and ApoE proteins further highlight its involvement in lipid regulation within neurodegenerative conditions. Understanding TREM2’s role can pave the way for targeted therapeutic approaches in these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (ab305103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (ab305103)

    Immunohistochemical analysis of paraffin-embedded (A) RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cell pellet and (B) Neuro-2a (mouse neuroblastoma neuroblast) cell pellet, labeling TREM2 with ab305103 at 1/500 dilution (1.014 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.

    The section was incubated with ab305103 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Positive staining in RAW264.7 cell pellet was observed with no staining in Neuro-2a cell pellet. Secondary antibody only control: RAW264.7 cell pellet with only ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (ab305103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (ab305103)

    Immunohistochemical analysis of paraffin-embedded (A) FAD4T Transgenic mouse Alzheimer’s disease cerebrum and (B) Mouse normal cerebrum (labeling TREM2 with ab305103 at 1/500 (1.014 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on (A) Mouse Alzheimer’s disease cerebrum, no staining on (B) Mouse normal cerebrum. The section was incubated with ab305103 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (ab305103), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TREM2 antibody [EPR26210-1] (ab305103)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T cells transfected with a mouse TREM2 expression vector containing a GFP tag and (B) HEK-293T cells transfected with an empty vector containing a GFP tag (labeling TREM2 with ab305103 at 1/500 (1.014 µg/ml) followed by a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) was used.

    Positive staining on (A) HEK-293T cells transfected with a Trem2 expression vector containing a GFP tag. No staining on (B) HEK-293T cells transfected with empty vector containing a GFP tag. The section was incubated with ab305103 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Leica DS9800 (Bond™ Polymer Refine Detection) kit.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins

  • Western blot - Anti-TREM2 antibody [EPR26210-1] (ab305103), expandable thumbnail

    Western blot - Anti-TREM2 antibody [EPR26210-1] (ab305103)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    TREM2 is a glycoprotein of approximately 35-50 kDa and detected as a 25 kDa band after treated with Protein Deglycosylation MIX II.
    The identity of the higher MW band at approximately 70 kDa is unknown.

    All lanes: Western blot - Anti-TREM2 antibody [EPR26210-1] (ab305103) at 1/1000 dilution

    Lane 1: Untreated J774A.1 (mouse reticum cell sarcoma monocyte macrophage), whole cell lysate at 30 µg

    Lane 2: J774A.1 whole cell lysate treated with Protein Deglycosylation Mix II at 30 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 35-50 kDa, 25 kDa

    Observed band size: 35-50 kDa, 25 kDa

    Exposure time: 3min

  • Western blot - Anti-TREM2 antibody [EPR26210-1] (ab305103), expandable thumbnail

    Western blot - Anti-TREM2 antibody [EPR26210-1] (ab305103)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The expression profile and molecular weight observed are consistent with what has been described in the literature (PMID: 29611543; PMID: 28559417).
    Negative control: L929, F9 (PMID: 11241283), Neuro-2a (PMID: 19302484).
    The identity of the higher MW band at approximately 70 kDa is unknown.

    All lanes: Western blot - Anti-TREM2 antibody [EPR26210-1] (ab305103) at 1/1000 dilution

    Lane 1: J774A.1 (mouse reticum cell sarcoma monocyte macrophage), whole cell lysate at 20 µg

    Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

    Lane 3: N9 (mouse microglia), whole cell lysate at 20 µg

    Lane 4: L929 (mouse connective tissue fibroblast), whole cell lysate at 20 µg

    Lane 5: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

    Lane 6: F9 (mouse embryonal carcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 25 kDa

    Observed band size: 35-50 kDa

    Exposure time: 3min

  • Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR26210-1] (ab305103), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TREM2 antibody [EPR26210-1] (ab305103)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) cells labelling TREM2 with ab305103 at 1/500 (1.014 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/mL dilution (Green). Confocal image showing cytoplasmic staining on RAW 264.7 cell line.Negative control: Neuro-2a (PMID: 19302484).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

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Product protocols

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