Rabbit Polyclonal Treslin antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TICRR aa 950-1050.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human TICRR aa 950-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z2Z1
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Chimpanzee | Predicted |
| Chinese hamster | Predicted |
| Gorilla | Predicted |
| Horse | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/2500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Horse, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating in CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation.
Alternative names
C15orf42, TICRR, Treslin, TopBP1-interacting checkpoint and replication regulator
Recommended products
Rabbit Polyclonal Treslin antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TICRR aa 950-1050.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human TICRR aa 950-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q7Z2Z1
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab124268 was affinity purified using an epitope specific to Treslin immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Treslin also known as TICRR is a protein that plays an important role in initiating DNA replication. It has a molecular mass of approximately 220 kDa. Treslin is expressed in various human tissues with significant levels observed in cell types that actively undergo division. Mechanically Treslin primarily functions in the S phase of the cell cycle enabling the transition from the G1 phase to the S phase. It does so by interacting with other replication factors facilitating the assembly of the pre-replicative complex necessary to commence DNA synthesis.
- Biological function summary
Treslin holds a significant position in the progression of the cell cycle. It participates in complex formation with other proteins such as TopBP1 where it supports replication initiation by recruiting and stabilizing additional replication machinery components. Treslin is necessary for the loading of Cdc45 onto replication origins. It ensures precise control over DNA replication preventing unscheduled or erroneous replication events which can lead to genomic instability.
- Pathways
Treslin operates in pivotal DNA replication and cell cycle control pathways. It works closely with proteins like Cdc45 and TopBP1 within the DNA replication start pathway and the cell cycle's S-phase entry process. Treslin's interactions with these proteins ensure that DNA replication starts accurately within the cell cycle framework thereby maintaining genomic integrity and proper cell function.
- Associated diseases and disorders
Researchers associate Treslin with cancer development and progression. Its malfunction or aberrant expression can result in genomic instability promoting oncogenesis. Treslin's interaction with TopBP1 also ties it to disorders involving cellular cycle dysregulation. Understanding Treslin's role in these disease pathways may help design therapeutic strategies that target replication machinery components to inhibit cancer cell proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
This image is courtesy of an anonymous customer review.Western blot - Anti-Treslin antibody (ab124268)
Blocking: 5% milkAll lanes: Western blot - Anti-Treslin antibody (ab124268) at 1/1000 dilution
Lane 1: 293 whole cell lysate treated with control siLUC RNAi at 50 µg
Lanes 2 - 3: 293 whole cell lysate treated with RNAi targeting Treslin at 50 µg
SecondaryAll lanes: Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (Donkey Anti-Rabbit IgG H&L (HRP) ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 211 kDa
Observed band size: 130 kDa, 250 kDa
Exposure time: 2min
Western blot - Anti-Treslin antibody (ab124268)
All lanes: Western blot - Anti-Treslin antibody (ab124268) at 0.4 µg/mL
Lane 1: 293T whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 211 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com