AB124268

Anti-Treslin antibody

Name: Anti-Treslin antibody (ab124268) | Abcam
Brand: Abcam Limited
SKU: AB124268
Rating: 4 (1 reviews)

(1 Review)

(3 Publications)

Rabbit Polyclonal Treslin antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TICRR aa 950-1050.

View Alternative Names

C15orf42, TICRR, Treslin, TopBP1-interacting checkpoint and replication regulator

2 Images

Collaborator

Western blot - Anti-Treslin antibody (AB124268)

Blocking : 5% milk

All lanes:

Western blot - Anti-Treslin antibody (ab124268) at 1/1000 dilution

Lane 1:

293 whole cell lysate treated with control siLUC RNAi at 50 µg

Lanes 2 - 3:

293 whole cell lysate treated with RNAi targeting Treslin at 50 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/donkey-rabbit-igg-h-l-hrp-ab16284'>ab16284</a>) at 1/20000 dilution

Predicted band size: 211 kDa

Observed band size: 130 kDa,250 kDa

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Exposure time: 2min

This image is courtesy of an anonymous abreview.

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Western blot - Anti-Treslin antibody (AB124268)

All lanes:

Western blot - Anti-Treslin antibody (ab124268) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 15 µg

Predicted band size: 211 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human TICRR aa 950-1050. The exact immunogen used to generate this antibody is proprietary information.

Q7Z2Z1

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab124268 was affinity purified using an epitope specific to Treslin immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Treslin also known as TICRR is a protein that plays an important role in initiating DNA replication. It has a molecular mass of approximately 220 kDa. Treslin is expressed in various human tissues with significant levels observed in cell types that actively undergo division. Mechanically Treslin primarily functions in the S phase of the cell cycle enabling the transition from the G1 phase to the S phase. It does so by interacting with other replication factors facilitating the assembly of the pre-replicative complex necessary to commence DNA synthesis.

Biological function summary

Treslin holds a significant position in the progression of the cell cycle. It participates in complex formation with other proteins such as TopBP1 where it supports replication initiation by recruiting and stabilizing additional replication machinery components. Treslin is necessary for the loading of Cdc45 onto replication origins. It ensures precise control over DNA replication preventing unscheduled or erroneous replication events which can lead to genomic instability.

Pathways

Treslin operates in pivotal DNA replication and cell cycle control pathways. It works closely with proteins like Cdc45 and TopBP1 within the DNA replication start pathway and the cell cycle's S-phase entry process. Treslin's interactions with these proteins ensure that DNA replication starts accurately within the cell cycle framework thereby maintaining genomic integrity and proper cell function.

Researchers associate Treslin with cancer development and progression. Its malfunction or aberrant expression can result in genomic instability promoting oncogenesis. Treslin's interaction with TopBP1 also ties it to disorders involving cellular cycle dysregulation. Understanding Treslin's role in these disease pathways may help design therapeutic strategies that target replication machinery components to inhibit cancer cell proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Regulator of DNA replication and S/M and G2/M checkpoints. Regulates the triggering of DNA replication initiation via its interaction with TOPBP1 by participating in CDK2-mediated loading of CDC45L onto replication origins. Required for the transition from pre-replication complex (pre-RC) to pre-initiation complex (pre-IC). Required to prevent mitotic entry after treatment with ionizing radiation.

See full target information TICRR

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:7708 PubMed38565932

2024

RECQL4 is not critical for firing of human DNA replication origins.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Padayachy,Sotirios G Ntallis,Thanos D Halazonetis

The EMBO journal 42:e114131 PubMed37458194

2023

Novel role of DONSON in CMG helicase assembly during vertebrate DNA replication initiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yoshitami Hashimoto,Kota Sadano,Nene Miyata,Haruka Ito,Hirofumi Tanaka

Cell reports 42:111912 PubMed36640304

2023

Axonal plasticity in response to active forces generated through magnetic nano-pulling.

Applications

Unspecified application

Species

Unspecified reactive species

Alessandro Falconieri,Sara De Vincentiis,Valentina Cappello,Domenica Convertino,Ravi Das,Samuele Ghignoli,Sofia Figoli,Stefano Luin,Frederic Català-Castro,Laura Marchetti,Ugo Borello,Michael Krieg,Vittoria Raffa

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Treslin antibody

Western blot - Anti-Treslin antibody (AB124268)

Western blot - Anti-Treslin antibody (AB124268)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

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