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AB1423

Anti-TRF1 antibody

2

(4 Reviews)

|

(45 Publications)

Rabbit Polyclonal TRF1 antibody. Suitable for ICC and reacts with Human samples. Cited in 45 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TERF1.

View Alternative Names

PIN2, TRBF1, TRF, TRF1, TERF1, Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1

2 Images
Immunocytochemistry - Anti-TRF1 antibody (AB1423)
  • ICC

Supplier Data

Immunocytochemistry - Anti-TRF1 antibody (AB1423)

The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

Immunocytochemistry - Anti-TRF1 antibody (AB1423)
  • ICC

Unknown

Immunocytochemistry - Anti-TRF1 antibody (AB1423)

Cells transfected with a TRF1-GFP contruct. On the left : immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre : fluorescence from the GFP. Right : merge of both images showing overlap of GFP fluorescence and antibody staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TERF1.

P54274

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
pH: 7.2 Preservative: 0.05% Sodium azide
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRF1 also known as TERF1 (Telomeric Repeat Binding Factor 1) is an important protein involved in telomere maintenance. This protein has a molecular mass of approximately 55 kDa. TRF1 localizes specifically to telomeres and plays an essential role in shelterin complex formation a specialized protein complex that protects chromosome ends. TRF1 is highly expressed in proliferating cells and tissues especially those with a high rate of cell division.
Biological function summary

TRF1 contributes to the stabilization of telomere structure and regulates telomere length. TRF1 is a part of the shelterin complex which also includes proteins such as TRF2 POT1 TIN2 RAP1 and TPP1. The shelterin complex prevents the telomeres from being recognized as DNA damage and regulates their replicative processes. TRF1 interacts directly with telomeric DNA influencing replication and modulating access to telomerase the enzyme responsible for telomere elongation.

Pathways

TRF1 plays a significant role in telomere maintenance and the cell cycle. The protein is critical in the DNA damage response pathway where it prevents unwanted DNA repair activities at telomeres. In this context TRF1 interacts with proteins like ATM and ATR which are important for signaling and responding to DNA damage. TRF1's interaction with these pathways helps maintain genomic stability and cellular homeostasis.

Alterations in TRF1 function can contribute to cancer and aging-related diseases. High levels or overexpression of TRF1 are frequently observed in certain cancers where it may help tumor cells maintain telomeres and proliferate indefinitely. Conversely lower TRF1 levels are associated with premature aging disorders such as dyskeratosis congenita. In these conditions the connection between TRF1 and TRF2 within the shelterin complex plays an important role in maintaining telomere integrity affecting disease development and progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length (PubMed : 31595153). Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
See full target information TERF1
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Publications (45)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40498073

2025

R-2-hydroxyglutarate-mediated inhibition of KDM4A compromises telomere integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Florence Couteau,Laurence M Gagné,Karine Boulay,Philippe Rousseau,Mélissa Carbonneau,Mary McQuaid,Jyoti Sharma,Christina Sawchyn,Erlinda Fernandez,Dagmar Glatz,Rana Rizk,Marie-Eve Lalonde,Yosra Mehrjoo,Tsz Wai Chu,Gaël Moquin-Beaudry,Christian Beauséjour,Mikhail Sergeev,Santiago Costantino,Daina Avizonis,Ivan Topisirovic,Nada Jabado,Hugo Wurtele,Chantal Autexier,Frédérick A Mallette

Cell communication and signaling : CCS 22:357 PubMed38987851

2024

Macrophage-derived exosomes promote telomere fragility and senescence in tubular epithelial cells by delivering miR-155.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Yin,Tao-Tao Tang,Xiao-Yu Lu,Wei-Jie Ni,Di Yin,Yi-Lin Zhang,Wei Jiang,Yue Zhang,Zuo-Lin Li,Yi Wen,Wei-Hua Gan,Ai-Qing Zhang,Lin-Li Lv,Bin Wang,Bi-Cheng Liu

FASEB bioAdvances 6:53-71 PubMed38344410

2024

Club cell-specific telomere protection protein 1 (TPP1) protects against tobacco smoke-induced lung inflammation, xenobiotic metabolic dysregulation, and injurious responses.

Applications

Unspecified application

Species

Unspecified reactive species

Thivanka Muthumalage,Chiara Goracci,Irfan Rahman

Molecular systems biology 19:e11267 PubMed37259925

2023

A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availability.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Moretton,Savvas Kourtis,Antoni Gañez Zapater,Chiara Calabrò,Maria Lorena Espinar Calvo,Frédéric Fontaine,Evangelia Darai,Etna Abad Cortel,Samuel Block,Laura Pascual-Reguant,Natalia Pardo-Lorente,Ritobrata Ghose,Matthew G Vander Heiden,Ana Janic,André C Müller,Joanna I Loizou,Sara Sdelci

Theranostics 12:4753-4766 PubMed35832084

2022

MiR-155 deficiency protects renal tubular epithelial cells from telomeric and genomic DNA damage in cisplatin-induced acute kidney injury.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Yin,Ya-Jie Zhao,Wei-Jie Ni,Tao-Tao Tang,Yao Wang,Jing-Yuan Cao,Di Yin,Yi Wen,Zuo-Lin Li,Yi-Lin Zhang,Wei Jiang,Yue Zhang,Xiao-Yu Lu,Ai-Qing Zhang,Wei-Hua Gan,Lin-Li Lv,Bi-Cheng Liu,Bin Wang

Methods in molecular biology (Clifton, N.J.) 2444:141-159 PubMed35290636

2022

Targeted Formation of 8-Oxoguanine in Telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Ryan P Barnes,Sanjana A Thosar,Elise Fouquerel,Patricia L Opresko

Nature communications 13:760 PubMed35140211

2022

Prime editing efficiency and fidelity are enhanced in the absence of mismatch repair.

Applications

Unspecified application

Species

Unspecified reactive species

J Ferreira da Silva,G P Oliveira,E A Arasa-Verge,C Kagiou,A Moretton,G Timelthaler,J Jiricny,J I Loizou

IET nanobiotechnology 16:14-24 PubMed34862858

2021

Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis by reducing the polarisation of M1 and M2 macrophages through the activation of EP2 receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Lu,Lulu Yang,Xiao Chen,Jing Liu,Anqi Nie,Xiaolan Chen

Metallomics : integrated biometal science 13: PubMed34021581

2021

Pt-ttpy, a G-quadruplex binding platinum complex, induces telomere dysfunction and G-rich regions DNA damage.

Applications

ChIP

Species

Human

Samar Ali,Emilia Puig Lombardi,Deepanjan Ghosh,Tao Jia,Géraldine Vitry,Lina Saker,Joël Poupon,Marie-Paule Teulade-Fichou,Alain Nicolas,Arturo Londono-Vallejo,Sophie Bombard

RNA biology 18:2261-2277 PubMed33749516

2021

Transcriptional regulation of telomeric repeat-containing RNA by acridine derivatives.

Applications

WB

Species

Human

Shuangshuang Kang,Jiaojiao Cao,Meiling Zhang,Xiaoya Li,Qian-Liang Guo,Huang Zeng,Zuzhuang Wei,Xue Gong,Jing Wang,Bobo Liu,Bing Shu,Xiaoli Xu,Zhi-Shu Huang,Ding Li
View all publications
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Product promise

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