Rabbit Monoclonal TRF1 antibody. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-TRF1 antibody [EPR28580-71] (AB323380), expandable thumbnail](https://content.abcam.com/products/images/trf1-antibody-epr28580-71-ab323380--western-blot-img441607.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRF1 antibody [EPR28580-71] (AB323380)")
![Western blot - Anti-TRF1 antibody [EPR28580-71] (AB323380), expandable thumbnail](https://content.abcam.com/products/images/trf1-antibody-epr28580-71-ab323380--western-blot-img441606.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-TRF1 antibody [EPR28580-71] (AB323380)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Alternative names
PIN2, TRBF1, TRF, TRF1, TERF1, Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1
Rabbit Monoclonal TRF1 antibody. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28580-71
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380), expandable thumbnail](https://content.abcam.com/products/images/trf1-antibody-epr28580-71-ab323380--western-blot-img441607.jpg "Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380)")
Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This antibody was tested on frozen and fresh HeLa and Jurkat whole cell lysate and it was concluded that TRF1 is the easy target for degradation.
The bands beneath the target band are likely to be degraded target fragments.
To minimize protein degradation, cells (lanes 7-8) were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
Exposure time: Lanes 1-6: 48 seconds; Lanes 7-8: 92 seconds
All lanes: Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380) at 1/1000 dilution
Lanes 1 and 7: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lanes 3 and 8: Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg
Lane 4: LN-229 (human brain glioblastoma epithelial cell) whole cell lysate at 20 µg
Lane 5: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg
Lane 6: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa, 36 kDa
![Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380), expandable thumbnail](https://content.abcam.com/products/images/trf1-antibody-epr28580-71-ab323380--western-blot-img441606.jpg "Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380)")
Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The bands beneath the target band are likely to be degraded target fragments.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-TRF1 antibody [EPR28580-71] (ab323380) at 1/1000 dilution
Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg
Lane 2: HeLa transfected with siRNA specifically targeting TRF1 whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 60 kDa, 36 kDa
Exposure time: 48s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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