Anti-TRF2 antibody [4A794]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] (AB13579)

ab13579 staining TRF2 in human bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-TRF2 antibody [4A794] (AB13579)

Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [4A794] (AB13579)

ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRF2 antibody [4A794] (AB13579)

Flow Cytometry (Intracellular) analysis of Jurkat cells labeling RF2 with ab13579 (red). The shaded histogram represents cells alone and blue represents isotype control.

• WB

Supplier Data

Western blot - Anti-TRF2 antibody [4A794] (AB13579)

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Western blot - Anti-TRF2 antibody [4A794] (ab13579) at 2 µg/mL

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Jurkat cell lysate

Secondary

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Goat anti-mouse Ig HRP

Predicted band size: 56 kDa

false

• WB

Supplier Data

Western blot - Anti-TRF2 antibody [4A794] (AB13579)

All lanes:

Western blot - Anti-TRF2 antibody [4A794] (ab13579)

All lanes:

HeLa cell lysate

Predicted band size: 56 kDa

false

• WB

Unknown

Western blot - Anti-TRF2 antibody [4A794] (AB13579)

Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

All lanes:

Western blot - Anti-TRF2 antibody [4A794] (ab13579)

Predicted band size: 56 kDa

false