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AB108997

Anti-TRF2 antibody [EPR3517(2)]

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(18 Publications)

Rabbit Recombinant Monoclonal TRF2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 18 publications.

View Alternative Names

TRBF2, TRF2, TERF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

ab108997 at 1/250 dilution staining TRF2 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

This image was generated using the unpurified version of the product.

Flow Cytometry (Intracellular) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRF2 with Purified ab108997 at 1/300 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TRF2 with Purified ab108997 at 1 : 1000 dilution (3.4 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TRF2 with Purified ab108997 at 1 : 300 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

ab108997 at 1/100 dilution staining TRF2 in HeLa cells by Immunofluorescence.

This image was generated using the unpurified version of the product.

Western blot - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • WB

Lab

Western blot - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

Blocking and dilution buffer : 5%NFDM/TBST

All lanes:

Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

All lanes:

HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 56 kDa

Observed band size: 62 kDa

false

Western blot - Anti-TRF2 antibody [EPR3517(2)] (AB108997)
  • WB

Unknown

Western blot - Anti-TRF2 antibody [EPR3517(2)] (AB108997)

This image was generated using the unpurified version of the product.

All lanes:

Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

Lane 1:

HT29 cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Predicted band size: 56 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3517(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/300", "ICCIF-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/300", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/250 - 1/500.</strong></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRF2 also known as Telomeric Repeat-binding Factor 2 is a protein important for telomere protection and maintenance. TRF2 weighs approximately 55 kDa and is widely expressed in human tissues especially in those with high turnover rates. This protein binds specifically to double-stranded telomeric DNA and forms a protective cap at the end of chromosomes helping to regulate telomere length and integrity. By securing the telomere's structure TRF2 prevents chromosomes from being recognized as damaged DNA.
Biological function summary

TRF2 plays a central role in telomere function by assembling into the shelterin complex. This complex which includes other proteins like TRF1 TIN2 POT1 TPP1 and RAP1 is essential for controlling telomere length and stability. TRF2 is responsible for inhibiting DNA damage response pathways at telomeres ensuring that the natural chromosomal ends are not mistaken for double-strand breaks. This suppression of unwanted DNA damage response is important for normal cell division and avoiding cellular senescence.

Pathways

Researchers have placed TRF2 within the DNA repair and telomere maintenance pathways. TRF2 interacts with several other proteins such as Ku70/80 and RAD51 that are instrumental in the DNA repair process. Furthermore TRF2 influences the Alternative Lengthening of Telomeres (ALT) pathway which is a telomere lengthening process found in some cancer cells. These connections highlight TRF2's role in maintaining genomic stability through its capacity to regulate telomere-associated proteins and pathways.

TRF2 has been linked to cancer and aging. Elevated levels of TRF2 have been detected in various cancers including breast and prostate cancer suggesting a role in tumorigenesis where it contributes to the stability of cancer cell telomeres. Additionally defects in TRF2 or its interaction with partner proteins like POT1 can lead to dysfunctional telomeres which contribute to the aging process and age-related diseases by affecting cell proliferation and survival. These links demonstrate the importance of TRF2 in normal cellular function and its potential as a therapeutic target in disease intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes (PubMed : 15608617, PubMed : 16166375, PubMed : 20655466, PubMed : 28216226, PubMed : 9326950, PubMed : 9326951, PubMed : 9476899). In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo (PubMed : 16166375, PubMed : 20655466). Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection (PubMed : 16166375). Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways (PubMed : 16166375). Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres : T loops have been proposed to protect chromosome ends from degradation and repair (PubMed : 20655466). Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo (PubMed : 20655466, PubMed : 28216226). Preferentially binds to positive supercoiled DNA (PubMed : 15608617, PubMed : 20655466). Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology (PubMed : 20655466). Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length (By similarity).
See full target information TERF2
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 20:7 PubMed39815362

2025

Bi-targeting of thioredoxin 1 and telomerase by thiotert promotes cell death of myelodysplastic syndromes and lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qiangan Jing,Yunyi Wu,Yanchun Li,Chaoting Zhou,Junyu Zhang,Jun Xia,Keyi Li,Yuhuan Shen,Hongfeng Yao,Xiangmin Tong,Jing Du,Lushan Yu,Ying Wang

Antioxidants (Basel, Switzerland) 13: PubMed39456511

2024

α-Terpineol Induces Shelterin Components TRF1 and TRF2 to Mitigate Senescence and Telomere Integrity Loss via A Telomerase-Independent Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Marianna Kapetanou,Sophia Athanasopoulou,Andreas Goutas,Dimitra Makatsori,Varvara Trachana,Efstathios Gonos

The Journal of experimental medicine 221: PubMed39297884

2024

Inhibition of hTERT/telomerase/telomere mediates therapeutic efficacy of osimertinib in EGFR mutant lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Chen,Karin A Vallega,Dongsheng Wang,Zihan Quan,Songqing Fan,Qiming Wang,Ticiana Leal,Suresh S Ramalingam,Shi-Yong Sun

Molecular systems biology 19:e11267 PubMed37259925

2023

A metabolic map of the DNA damage response identifies PRDX1 in the control of nuclear ROS scavenging and aspartate availability.

Applications

Unspecified application

Species

Unspecified reactive species

Amandine Moretton,Savvas Kourtis,Antoni Gañez Zapater,Chiara Calabrò,Maria Lorena Espinar Calvo,Frédéric Fontaine,Evangelia Darai,Etna Abad Cortel,Samuel Block,Laura Pascual-Reguant,Natalia Pardo-Lorente,Ritobrata Ghose,Matthew G Vander Heiden,Ana Janic,André C Müller,Joanna I Loizou,Sara Sdelci

iScience 26:106158 PubMed36843839

2023

Telomeres cooperate in zygotic genome activation by affecting / transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Zhang,Changquan Zhang,Di Zhou,Tianlei Zhang,Xueqin Chen,Jinlin Ren,Caixia He,Fei Meng,Qinwei Zhou,Qiaohui Yang,Congling Dai,Ge Lin,Sicong Zeng,Lizhi Leng

Cell death & disease 13:1005 PubMed36437244

2022

Teloxantron inhibits the processivity of telomerase with preferential DNA damage on telomeres.

Applications

Unspecified application

Species

Unspecified reactive species

Natalia Maciejewska,Mateusz Olszewski,Jakub Jurasz,Maciej Baginski,Maryna Stasevych,Viktor Zvarych,Marco Folini,Nadia Zaffaroni

STAR protocols 3:101610 PubMed36035793

2022

An optimized proximity ligation assay to detect telomere dysfunction induced foci in human and mouse cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yajun Wang,Luigi Ferrucci,Michael M Seidman,Yie Liu

Journal of bone oncology 36:100448 PubMed35942470

2022

RNA 5-methylcytosine status is associated with DNMT2/TRDMT1 nuclear localization in osteosarcoma cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Betlej,Tomasz Ząbek,Anna Lewińska,Dominika Błoniarz,Iwona Rzeszutek,Maciej Wnuk

Biochemical and biophysical research communications 579:141-145 PubMed34600299

2021

SARS-CoV-2 triggers DNA damage response in Vero E6 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua Victor,Jamie Deutsch,Annalis Whitaker,Erica N Lamkin,Anthony March,Pei Zhou,Jason W Botten,Nimrat Chatterjee

Breast cancer research : BCR 23:89 PubMed34488828

2021

TAZ maintains telomere length in TNBC cells by mediating Rad51C expression.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Yang,Bo Wang,Xinyan Jiao,Can Zhou,Su Chen,Xiaoqian Gao,Wei Sun,Shaoran Song,Juan Li,Jie Liu,Yaochun Wang,Peijun Liu
View all publications
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Product promise

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