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Rabbit Recombinant Monoclonal TRF2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 13 publications.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (AB108997), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (AB108997), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TRF2 antibody [EPR3517(2)] (AB108997), expandable thumbnail
  • Western blot - Anti-TRF2 antibody [EPR3517(2)] (AB108997), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (AB108997), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Not recommended
Tested
Tested
Tested
Tested

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/300
Notes

For unpurified use at 1/100 - 1/250.

Tested
Tested

Species
Human
Dilution info
1/300
Notes

-

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes (PubMed:15608617, PubMed:16166375, PubMed:20655466, PubMed:28216226, PubMed:9326950, PubMed:9326951, PubMed:9476899). In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo (PubMed:16166375, PubMed:20655466). Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection (PubMed:16166375). Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways (PubMed:16166375). Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair (PubMed:20655466). Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo (PubMed:20655466, PubMed:28216226). Preferentially binds to positive supercoiled DNA (PubMed:15608617, PubMed:20655466). Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology (PubMed:20655466). Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length (By similarity).

Alternative names

TRBF2, TRF2, TERF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

Recommended products

Rabbit Recombinant Monoclonal TRF2 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 13 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3517(2)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRF2 also known as Telomeric Repeat-binding Factor 2 is a protein important for telomere protection and maintenance. TRF2 weighs approximately 55 kDa and is widely expressed in human tissues especially in those with high turnover rates. This protein binds specifically to double-stranded telomeric DNA and forms a protective cap at the end of chromosomes helping to regulate telomere length and integrity. By securing the telomere's structure TRF2 prevents chromosomes from being recognized as damaged DNA.

Biological function summary

TRF2 plays a central role in telomere function by assembling into the shelterin complex. This complex which includes other proteins like TRF1 TIN2 POT1 TPP1 and RAP1 is essential for controlling telomere length and stability. TRF2 is responsible for inhibiting DNA damage response pathways at telomeres ensuring that the natural chromosomal ends are not mistaken for double-strand breaks. This suppression of unwanted DNA damage response is important for normal cell division and avoiding cellular senescence.

Pathways

Researchers have placed TRF2 within the DNA repair and telomere maintenance pathways. TRF2 interacts with several other proteins such as Ku70/80 and RAD51 that are instrumental in the DNA repair process. Furthermore TRF2 influences the Alternative Lengthening of Telomeres (ALT) pathway which is a telomere lengthening process found in some cancer cells. These connections highlight TRF2's role in maintaining genomic stability through its capacity to regulate telomere-associated proteins and pathways.

Associated diseases and disorders

TRF2 has been linked to cancer and aging. Elevated levels of TRF2 have been detected in various cancers including breast and prostate cancer suggesting a role in tumorigenesis where it contributes to the stability of cancer cell telomeres. Additionally defects in TRF2 or its interaction with partner proteins like POT1 can lead to dysfunctional telomeres which contribute to the aging process and age-related diseases by affecting cell proliferation and survival. These links demonstrate the importance of TRF2 in normal cellular function and its potential as a therapeutic target in disease intervention.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling TRF2 with Purified ab108997 at 1:1000 dilution (3.4 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling TRF2 with Purified ab108997 at 1:300 dilution (10 μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling TRF2 with Purified ab108997 at 1/300 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    Blocking and dilution buffer: 5%NFDM/TBST

    All lanes: Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

    All lanes: HT-29 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa

    Observed band size: 62 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    ab108997 at 1/100 dilution staining TRF2 in HeLa cells by Immunofluorescence.

    This image was generated using the unpurified version of the product.

  • Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    This image was generated using the unpurified version of the product.

    All lanes: Western blot - Anti-TRF2 antibody [EPR3517(2)] (ab108997) at 1/1000 dilution

    Lane 1: HT29 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRF2 antibody [EPR3517(2)] (ab108997)

    ab108997 at 1/250 dilution staining TRF2 in Human spleen by Immunohistochemistry, Paraffin-embedded tissue.

    This image was generated using the unpurified version of the product.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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