AB108932

Anti-TRF2 antibody [EPR3518(2)]

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal TRF2 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

TRBF2, TRF2, TERF2, Telomeric repeat-binding factor 2, TTAGGG repeat-binding factor 2, Telomeric DNA-binding protein

1 Images

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Western blot - Anti-TRF2 antibody [EPR3518(2)] (AB108932)

All lanes:

Western blot - Anti-TRF2 antibody [EPR3518(2)] (ab108932) at 1/1000 dilution

Lane 1:

Jurkat cell lysate at 10 µg

Lane 2:

NAMALWA cell lysate at 10 µg

Predicted band size: 56 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3518(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRF2 also known as Telomeric Repeat-binding Factor 2 is a protein important for telomere protection and maintenance. TRF2 weighs approximately 55 kDa and is widely expressed in human tissues especially in those with high turnover rates. This protein binds specifically to double-stranded telomeric DNA and forms a protective cap at the end of chromosomes helping to regulate telomere length and integrity. By securing the telomere's structure TRF2 prevents chromosomes from being recognized as damaged DNA.

Biological function summary

TRF2 plays a central role in telomere function by assembling into the shelterin complex. This complex which includes other proteins like TRF1 TIN2 POT1 TPP1 and RAP1 is essential for controlling telomere length and stability. TRF2 is responsible for inhibiting DNA damage response pathways at telomeres ensuring that the natural chromosomal ends are not mistaken for double-strand breaks. This suppression of unwanted DNA damage response is important for normal cell division and avoiding cellular senescence.

Pathways

Researchers have placed TRF2 within the DNA repair and telomere maintenance pathways. TRF2 interacts with several other proteins such as Ku70/80 and RAD51 that are instrumental in the DNA repair process. Furthermore TRF2 influences the Alternative Lengthening of Telomeres (ALT) pathway which is a telomere lengthening process found in some cancer cells. These connections highlight TRF2's role in maintaining genomic stability through its capacity to regulate telomere-associated proteins and pathways.

TRF2 has been linked to cancer and aging. Elevated levels of TRF2 have been detected in various cancers including breast and prostate cancer suggesting a role in tumorigenesis where it contributes to the stability of cancer cell telomeres. Additionally defects in TRF2 or its interaction with partner proteins like POT1 can lead to dysfunctional telomeres which contribute to the aging process and age-related diseases by affecting cell proliferation and survival. These links demonstrate the importance of TRF2 in normal cellular function and its potential as a therapeutic target in disease intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes (PubMed : 15608617, PubMed : 16166375, PubMed : 20655466, PubMed : 28216226, PubMed : 9326950, PubMed : 9326951, PubMed : 9476899). In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo (PubMed : 16166375, PubMed : 20655466). Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection (PubMed : 16166375). Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways (PubMed : 16166375). Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres : T loops have been proposed to protect chromosome ends from degradation and repair (PubMed : 20655466). Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo (PubMed : 20655466, PubMed : 28216226). Preferentially binds to positive supercoiled DNA (PubMed : 15608617, PubMed : 20655466). Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology (PubMed : 20655466). Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length (By similarity).

See full target information TERF2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 15:9819-9837 PubMed41041071

2025

Endothelial cell-derived SDF-1α elicits stemness traits of glioblastoma dual-regulation of GLI1.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Yuan,Xudong Liu,Liwen Kuang,Shixue Yang,Lihong Wang,Jiao Wang,Sen Wei,Zexuan Yan,Qinghua Ma,Juan Lei,Yu Zhou,Yu Chen,Jiongming Chen,Tao Luo,Kaidi Yang,Mengsi Zhang,Yongsheng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRF2 antibody [EPR3518(2)]

Western blot - Anti-TRF2 antibody [EPR3518(2)] (AB108932)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise