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Mouse Monoclonal TRF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 68 publications. Immunogen corresponding to Full Length Protein corresponding to Human Telomeric repeat-binding factor 1.


Images

Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579), expandable thumbnail
  • Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579), expandable thumbnail
  • Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (AB10579), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Human Telomeric repeat-binding factor 1. The exact immunogen used to generate this antibody is proprietary information. Database link P54274

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IF
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

2.00000-4.00000 µg/mL

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.

Targets

Telomeric repeat-binding factor 2

Alternative names

Recommended products

Mouse Monoclonal TRF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 68 publications. Immunogen corresponding to Full Length Protein corresponding to Human Telomeric repeat-binding factor 1.

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Human Telomeric repeat-binding factor 1. The exact immunogen used to generate this antibody is proprietary information. Database link P54274
Clone number

TRF-78

Purification technique

Affinity purification Protein G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRF2 and TRF1 also known as Telomeric Repeat-binding Factors 2 and 1 are proteins essential for telomere maintenance. TRF2 has a molecular mass of approximately 54 kDa while TRF1 is around 56 kDa. These proteins are ubiquitously expressed across various cell types with significant concentrations in proliferative tissues like bone marrow and testis. Both TRF2 and TRF1 are part of the shelterin complex a critical component in regulating telomere length and protecting chromosome ends.

Biological function summary

The shelterin complex which includes TRF2 and TRF1 plays a pivotal role in telomere integrity. They bind to telomeric DNA preventing end-to-end chromosome fusions and inappropriate activation of DNA damage responses. TRF2 and TRF1 are key to preserving telomere structure by facilitating t-loop configuration. This structural maintenance prevents deleterious genomic instability which could otherwise lead to cellular dysfunction or transformation.

Pathways

TRF2 and TRF1 are integral to the telomere signaling pathway. These proteins coordinate with other shelterin components and are essential in managing telomere-associated checkpoint pathways. TRF2 associates closely with proteins like RAP1 aiding in telomere protection and limiting telomerase access. TRF1 interacts with proteins like TIN2 and POT1 further influencing the telomere maintenance pathways by modulating telomeric DNA accessibility and structural organization.

Associated diseases and disorders

TRF2 and TRF1 are linked to cancer and aging-related conditions. Dysfunction in these proteins disrupts telomere homeostasis potentially leading to tumorigenesis due to unchecked cell division. In cancer abnormal expression of TRF2 connects to proteins such as ATM which play important roles in DNA damage response and repair pathways. Additionally alterations in TRF1 expression can accelerate cellular aging processes highlighting its significance in age-associated diseases.

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6 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

    Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TRF1 and TRF2 with ab10579. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 10 μg/mL Anti-TRF2 + TRF1 antibody [TRF-78]. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

  • Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579), expandable thumbnail
    Verified customer

    Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

    Blocking step
    Milk as blocking agent for 2 hours · Concentration: 5% · Temperature: 21°C.

    Incubation time
    12 hours · Temperature: 4°C · Diluent: 5% milk in TBST.

    All lanes: Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 1/1000 dilution

    Lane 1: SAOS2 (Human osteosarcoma cell line) cell lysate at 20 µg

    Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate at 20 µg

    Secondary

    All lanes: Goat anti Mouse polyclonal IRDye 800CW at 1/1000 dilution

    Predicted band size: 50 kDa

    Exposure time: 5min

  • Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579), expandable thumbnail

    Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

    All lanes: Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/mL

    Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate

    Lane 2: HeLa cell lysate

    Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Lane 4: U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate

    Lane 5: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate

    Secondary

    All lanes: Goat Anti-Mouse IgG-Peroxidase

    Predicted band size: 50 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579), expandable thumbnail
    This image is courtesy of Luke Hughes-Davies and Rhiannon Jade, Gurdon Institute, Cambridge, UK

    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

    Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody. A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation. The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.

    IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain.

  • Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579), expandable thumbnail
    This image is courtesy of an anonymous customer review

    Immunocytochemistry/ Immunofluorescence - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

    ab10579 at 1/500 staining human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.

  • Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579), expandable thumbnail

    Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579)

    All lanes: Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/mL

    Lane 1: Recombinant Human TRF1 protein cell lysate at 0.1 µg

    Lane 2: Recombinant Human TRF1 protein cell lysate at 0.5 µg

    Lane 3: Empty cell lysate

    Lane 4: Recombinant Human TRF2 protein cell lysate at 0.1 µg

    Lane 5: Recombinant Human TRF2 protein cell lysate at 0.5 µg

    Observed band size: 80 kDa, 56 kDa

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