Mouse Monoclonal TRF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 68 publications. Immunogen corresponding to Full Length Protein corresponding to Human Telomeric repeat-binding factor 1.
IgG1
Mouse
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS
Liquid
Monoclonal
WB | ICC/IF | |
---|---|---|
Human | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 2.00000-4.00000 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100 | Notes - |
Select an associated product type
Binds the telomeric double-stranded 5'-TTAGGG-3' repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Telomeric repeat-binding factor 2
PIN2, TRBF1, TRF, TRF1, TRF, TRF1, TRBF1, PIN2, TERF1, Telomeric repeat-binding factor 1, NIMA-interacting protein 2, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1
Mouse Monoclonal TRF1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 68 publications. Immunogen corresponding to Full Length Protein corresponding to Human Telomeric repeat-binding factor 1.
IgG1
Mouse
pH: 7.4
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS
Liquid
Monoclonal
TRF-78
Affinity purification Protein G
Blue Ice
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
TRF2 and TRF1 also known as Telomeric Repeat-binding Factors 2 and 1 are proteins essential for telomere maintenance. TRF2 has a molecular mass of approximately 54 kDa while TRF1 is around 56 kDa. These proteins are ubiquitously expressed across various cell types with significant concentrations in proliferative tissues like bone marrow and testis. Both TRF2 and TRF1 are part of the shelterin complex a critical component in regulating telomere length and protecting chromosome ends.
The shelterin complex which includes TRF2 and TRF1 plays a pivotal role in telomere integrity. They bind to telomeric DNA preventing end-to-end chromosome fusions and inappropriate activation of DNA damage responses. TRF2 and TRF1 are key to preserving telomere structure by facilitating t-loop configuration. This structural maintenance prevents deleterious genomic instability which could otherwise lead to cellular dysfunction or transformation.
TRF2 and TRF1 are integral to the telomere signaling pathway. These proteins coordinate with other shelterin components and are essential in managing telomere-associated checkpoint pathways. TRF2 associates closely with proteins like RAP1 aiding in telomere protection and limiting telomerase access. TRF1 interacts with proteins like TIN2 and POT1 further influencing the telomere maintenance pathways by modulating telomeric DNA accessibility and structural organization.
TRF2 and TRF1 are linked to cancer and aging-related conditions. Dysfunction in these proteins disrupts telomere homeostasis potentially leading to tumorigenesis due to unchecked cell division. In cancer abnormal expression of TRF2 connects to proteins such as ATM which play important roles in DNA damage response and repair pathways. Additionally alterations in TRF1 expression can accelerate cellular aging processes highlighting its significance in age-associated diseases.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling TRF1 and TRF2 with ab10579. Cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton™ X-100. Fixed cells were stained with 10 μg/mL Anti-TRF2 + TRF1 antibody [TRF-78]. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
Blocking step
Milk as blocking agent for 2 hours · Concentration: 5% · Temperature: 21°C.
Incubation time
12 hours · Temperature: 4°C · Diluent: 5% milk in TBST.
All lanes: Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 1/1000 dilution
Lane 1: SAOS2 (Human osteosarcoma cell line) cell lysate at 20 µg
Lane 2: U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate at 20 µg
All lanes: Goat anti Mouse polyclonal IRDye 800CW at 1/1000 dilution
Predicted band size: 50 kDa
Exposure time: 5min
All lanes: Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/mL
Lane 1: HeLa (Human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate
Lane 2: HeLa cell lysate
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 4: U-2 OS (Human bone osteosarcoma epithelial cell line) cell lysate
Lane 5: HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
All lanes: Goat Anti-Mouse IgG-Peroxidase
Predicted band size: 50 kDa
Immunofluorescent imaging of human cells (U2OS) with ab10579 confirms the specificity of this antibody. A few intense nuclear foci are seen in interphase cells, corresponding to telomeric localisation. The complete absence of background nuclear or cytoplasmic staining confirms the specificity of this antibody. This image is in exact agreement with numerous published reports.
IF was performed with a standard paraformaldehyde technique (fixed in PBS buffered PFH 4% for 5 minutes, permeabilised with 0.5% triton-PBS for 5 minutes, blocked with 5% milk / 0.2% tween for one hour. Primary antibody used at 1/100 in 5% milk / 0.2% TWEEN for one hour, secondary antibody for 30 minutes. All blocking and incubation steps carried out at 37 degrees. Nuclei are visualised using Hoechst stain.
ab10579 at 1/500 staining human HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF. The cells were parafomaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa Fluor® 555 conjugated donkey anti-mouse antibody was used as the secondary.
All lanes: Western blot - Anti-TRF2 + TRF1 antibody [TRF-78] (ab10579) at 4 µg/mL
Lane 1: Recombinant Human TRF1 protein cell lysate at 0.1 µg
Lane 2: Recombinant Human TRF1 protein cell lysate at 0.5 µg
Lane 3: Empty cell lysate
Lane 4: Recombinant Human TRF2 protein cell lysate at 0.1 µg
Lane 5: Recombinant Human TRF2 protein cell lysate at 0.5 µg
Observed band size: 80 kDa, 56 kDa
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