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AB67800

Anti-TRFP/MED20 antibody

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(1 Publication)

Mouse Polyclonal TRFP/MED20 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MED20.

View Alternative Names

TRFP, MED20, Mediator of RNA polymerase II transcription subunit 20, Mediator complex subunit 20, TRF-proximal protein homolog, hTRFP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TRFP/MED20 antibody (AB67800)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRFP/MED20 antibody (AB67800)

ab67800 at 10μg/ml staining TRFP/MED20 in Hela cells.

Western blot - Anti-TRFP/MED20 antibody (AB67800)
  • WB

Unknown

Western blot - Anti-TRFP/MED20 antibody (AB67800)

All lanes:

Western blot - Anti-TRFP/MED20 antibody (ab67800) at 1/500 dilution

Lane 1:

TRFP/MED20 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 23 kDa

Observed band size: 21 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MED20.

Q9H944

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRFP also known as MED20 is part of the Mediator complex which acts as a communication bridge between gene-specific regulatory proteins and RNA polymerase II. MED20 has a molecular mass of approximately 22 kDa and is essential for the transcriptional coactivation process. This protein is highly expressed in various human tissues including the liver heart and kidneys with detectable levels in several other organs. MED20 facilitates the activation of transcription by interacting with multiple transcription factors.
Biological function summary

The Mediator complex which includes MED20 as one of its subunits plays a significant role in the initiation of transcription. MED20 forms part of the larger Mediator complex working closely with other subunits to modulate gene expression. By serving as a scaffold it helps stabilize interactions between transcription factors and RNA polymerase II enhancing transcription efficiency. The complex influences cell growth differentiation and response to cellular signals.

Pathways

MED20's presence in the Mediator complex situates it in critical transcription regulation pathways such as the RNA polymerase II transcription initiation pathway. It interacts with proteins like CDK8 and CCNC integral in controlling transcription by regulating Mediator's cyclin-dependent kinase activity. MED20 fosters connections between regulatory proteins and RNA polymerase II affecting transcription-related cellular signaling.

Abnormalities in the Mediator complex including issues with MED20 have implications in certain disorders like cancer and cardiovascular disease. Dysregulation of gene expression through interrupted Mediator activity can lead to these conditions. Specifically deficiencies involving MED20 affect the overall integrity of the Mediator complex relating to proteins such as MED12 and MED1 which have associations with oncogenesis and developmental defects.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
See full target information MED20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Heliyon 9:e22086 PubMed38046167

2023

Long non-coding RNA PCAT5 regulates the progression of Esophageal Squamous Cell Carcinoma via miR-4295/PHF20.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Liu,Hang Yin,Tao Yang,Jiacai Lin,Tingting Sun
View all publications
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Product promise

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