AB72179

Anti-TRH-R antibody

Name: Anti-TRH-R antibody (ab72179) | Abcam
Brand: Abcam Limited
SKU: AB72179
Rating: 4 (2 reviews)

(2 Reviews)

(5 Publications )

Rabbit Polyclonal TRH-R antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Thyrotropin-releasing hormone receptor aa 350 to C-terminus.

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRH-R antibody (AB72179)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRH-R antibody (AB72179)

ab72179 at 10μg/ml staining TRH-R in human thyroid by Immunohistochemistry using paraffin-embedded tissue.

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Western blot - Anti-TRH-R antibody (AB72179)

All lanes:

Western blot - Anti-TRH-R antibody (ab72179) at 0.5 µg/mL

Lane 1:

Human brain lysate

Lane 2:

Human brain lysate with immunising peptide

Predicted band size: 45 kDa

Observed band size: 45 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human Thyrotropin-releasing hormone receptor aa 350 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P34981

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.2% Gelatin

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRH-R also known as Thyrotropin-Releasing Hormone Receptor is a G-protein-coupled receptor specifically binding the tripeptide hormone TRH. This receptor has a molecular weight of approximately 70 kDa. It is express in high levels in the anterior pituitary gland as well as the central nervous system and various peripheral tissues. TRH-R plays a mechanical role in mediating the effects of TRH which include stimulating the release of thyroid-stimulating hormone (TSH) and prolactin from the anterior pituitary.

Biological function summary

The TRH-R is involved in important physiological processes. The binding of TRH to TRH-R initiates a cascade of intracellular events leading to the activation of phospholipase C and the subsequent production of inositol triphosphate and diacylglycerol. This signaling pathway elevates intracellular calcium levels and ultimately modulates the secretion of hormones like TSH. TRH-R does not form part of a larger complex but relies on interaction with heterotrimeric G proteins to exert its effects.

Pathways

TRH-R is significantly involved in the hypothalamic-pituitary-thyroid axis. Upon activation by TRH TRH-R activates signaling pathways that regulate thyroid hormone levels playing a role in metabolism. It also interacts with other receptors such as somatostatin receptors which can modulate the inhibitory effects on hormone secretion processes.

The dysregulation of TRH-R function has links with conditions like hypothyroidism and hyperprolactinemia. In these disorders the altered signaling of TRH-R influences the levels of important hormones such as TSH and prolactin. TRH-R connects with thyrotropin and prolactin-related proteins which serves as both diagnostic markers and potential therapeutic targets in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for thyrotropin-releasing hormone (TRH). Upon ligand binding, this G-protein-coupled receptor triggers activation of the phosphatidylinositol (IP3)-calcium-protein kinase C (PKC) pathway.

See full target information Thyrotropin-releasing hormone receptor

Alternative Names

Thyrotropin-releasing hormone receptor, TRH-R, Thyroliberin receptor, TRHR

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed37686439

2023

Novel Correlation between TGF-β1/-β3 and Hormone Receptors in the Human Corneal Stroma.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander J Choi,Brenna S Hefley,Sarah E Nicholas,Rebecca L Cunningham,Dimitrios Karamichos

International journal of molecular sciences 21: PubMed32093298

2020

Thyrotropin-Releasing Hormone (TRH) and Somatostatin (SST), but not Growth Hormone-Releasing Hormone (GHRH) nor Ghrelin (GHRL), Regulate Expression and Release of Immune Growth Hormone (GH) from Chicken Bursal B-Lymphocyte Cultures.

Applications

Unspecified application

Species

Unspecified reactive species

Santiago Pech-Pool,Laura C Berumen,Carlos G Martínez-Moreno,Guadalupe García-Alcocer,Martha Carranza,Maricela Luna,Carlos Arámburo

The veterinary quarterly 38:72-78 PubMed30362899

2018

TRH-induced secretion of adrenocorticotropin and cortisol in dogs with pituitary-dependent hypercortisolism.

Applications

Unspecified application

Species

Unspecified reactive species

Tera Pijnacker,Marieke Knies,Sara Galac,Karin Sanders,Jan A Mol,Hans S Kooistra

Neuropeptides 70:47-54 PubMed29803395

2018

The potential role of thyrotropin-releasing hormone in colonic dysmotility induced by water avoidance stress in rats.

Applications

WB, IHC-P

Species

Unspecified reactive species, Unspecified reactive species

Yanzhen Zhang,Chunfeng Wang,Lianfeng Zhang

The Journal of physiology 593:415-30 PubMed25630262

2015

RNASeq-derived transcriptome comparisons reveal neuromodulatory deficiency in the CO₂ insensitive brown Norway rat.

Applications

Unspecified application

Species

Unspecified reactive species

Madeleine M Puissant,Ashley E Echert,Chun Yang,Gary C Mouradian,Tyler Novotny,Pengyuan Liu,Mingyu Liang,Matthew R Hodges

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRH-R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRH-R antibody (AB72179)

Western blot - Anti-TRH-R antibody (AB72179)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (5)

Product promise