Rabbit Polyclonal Triadin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Trdn aa 450 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Rat Trdn aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QX75
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Mouse | Tested | Expected |
| Rat | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.2-2 µg/mL | Notes - |
Species Rat | Dilution info 0.2-2 µg/mL | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 5-20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Contributes to the regulation of lumenal Ca2+ release via the sarcoplasmic reticulum calcium release channels RYR1 and RYR2, a key step in triggering skeletal and heart muscle contraction. Required for normal organization of the triad junction, where T-tubules and the sarcoplasmic reticulum terminal cisternae are in close contact. Required for normal skeletal muscle strength (PubMed:19843516). Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.
Alternative names
Triadin, Trdn
Recommended products
Rabbit Polyclonal Triadin antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Rat Trdn aa 450 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Rat Trdn aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9QX75
- Purification technique
- Affinity purification Protein A
- Concentration
- Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Triadin also known as TRDN is a protein that plays a mechanical role in muscle function. This protein with a molecular weight of approximately 95 kDa is an essential component of the junctional face membrane of the sarcoplasmic reticulum. Triadin expresses mainly in cardiac and skeletal muscle tissues. It participates actively in calcium handling acting as a scaffolding protein that anchors other essential proteins within the calcium release units.
- Biological function summary
Triadin facilitates excitation-contraction coupling by forming part of the macromolecular complex involved in calcium signaling. It is a binding partner in the calcium release channel complex interacting directly with the cardiac ryanodine receptor (RyR2) and calsequestrin. These interactions stabilize the channel and ensure efficient calcium release during muscle contraction. Triadin's role is important for maintaining cardiac rhythm and skeletal muscle function.
- Pathways
Triadin integrates into the calcium signaling and muscle contraction pathways. Within these pathways it directly interacts with proteins like calsequestrin and the ryanodine receptors. Triadin ensures proper release of stored calcium from the sarcoplasmic reticulum which is vital for muscle contractions. It holds significant connections with other proteins involved in these pathways contributing to the regulation of muscle physiology and calcium dynamics.
- Associated diseases and disorders
Disruptions in triadin function link to cardiac and muscular conditions. Triadin dysfunction or mutations can result in inherited arrhythmias such as catecholaminergic polymorphic ventricular tachycardia (CPVT). This condition prominently features irregular heartbeats that may lead to sudden cardiac arrest. Triadin also has a documented connection to calsequestrin where mutations in both proteins can cooperatively exacerbate calcium handling disorders. Understanding triadin's role can aid in diagnosing and potentially treating these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Triadin antibody (ab231058)
Formalin-fixed, paraffin-embedded rat skeletal muscle tissue stained for Triadin with ab231058 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Western blot - Anti-Triadin antibody (ab231058)
All lanes: Western blot - Anti-Triadin antibody (ab231058) at 2 µg/mL
All lanes: mouse heart lysate
Developed using the ECL technique.
Predicted band size: 82 kDa
Western blot - Anti-Triadin antibody (ab231058)
All lanes: Western blot - Anti-Triadin antibody (ab231058) at 2 µg/mL
All lanes: mouse skeletal muscle lysate
Developed using the ECL technique.
Predicted band size: 82 kDa
Western blot - Anti-Triadin antibody (ab231058)
All lanes: Western blot - Anti-Triadin antibody (ab231058) at 2 µg/mL
All lanes: recombinant rat Triadin protein
Developed using the ECL technique.
Predicted band size: 82 kDa
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