Rabbit Polyclonal TRIB3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIB3 aa 150 to C-terminus.
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- Scientific reports 12:145202022Local anesthetic lidocaine-inducible gene, growth differentiation factor-15 suppresses the growth of cancer cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeiko Haraguchi-Suzuki,Reika Kawabata-Iwakawa,Toru Suzuki,Takashi Suto,Tomonori Takazawa,Shigeru SaitoPubMed 36008442
- BMC cancer 22:5582022Laminin-integrin a6b4 interaction activates notch signaling to facilitate bladder cancer development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Hao,Daming Yang,Tianpei Liu,Shucheng Liu,Xinsheng Lu,Libo ChenPubMed 35585515
- Acta pharmaceutica Sinica. B 12:1240-12532022c-MYC-mediated TRIB3/P62 aggresomes accumulation triggers paraptosis upon the combination of everolimus and ginsenoside Rh2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMin-Xia Su,Yu-Lian Xu,Xiao-Ming Jiang,Mu-Yang Huang,Le-Le Zhang,Luo-Wei Yuan,Xiao-Huang Xu,Qi Zhu,Jian-Li Gao,Jia-Hong Lu,Xiuping Chen,Ming-Qing Huang,Yitao Wang,Jin-Jian LuPubMed 35530150
- Annals of translational medicine 9:12532021Tribbles pseudokinase 3 () contributes to the progression of hepatocellular carcinoma by activating the mitogen-activated protein kinase pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui-Qi Wang,Fa-Zhong He,Qian Meng,Wei-Jie Lin,Jia-Mei Dong,Hai-Kui Yang,Yang Yang,Min Zhao,Wen-Tao Qiu,Yong-Jie Xin,Zhi-Ling ZhouPubMed 34532390
- Acta pharmaceutica Sinica. B 11:3105-31192021TRIB3‒GSK-3 interaction promotes lung fibrosis and serves as a potential therapeutic target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShanshan Liu,Xiaoxi Lv,Xupeng Wei,Chang Liu,Qiao Li,Jiali Min,Fang Hua,Xiaowei Zhang,Ke Li,Pingping Li,Yang Xiao,Zhuowei Hu,Bing CuiPubMed 34729304
- Cancer biomarkers : section A of Disease markers 31:307-3152021TRIB3 promotes proliferation, migration, and invasion of retinoblastoma cells by activating the AKT/mTOR signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXian-Yi Bao,Ming Sun,Ting-Ting Peng,Dong-Mei HanPubMed 33896816
- Experimental and therapeutic medicine 21:3132021TRIB3 promotes oral squamous cell carcinoma cell proliferation by activating the AKT signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Shen,Tian-Yang Zhang,Shu-Yan WangPubMed 33717256
- Nature communications 11:63162020TRIB3 promotes MYC-associated lymphoma development through suppression of UBE3B-mediated MYC degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKe Li et. al.PubMed 33298911
- eLife 9:2020CBR regulates soluble leptin receptor levels via CHOP, contributing to hepatic leptin resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdi Drori et. al.PubMed 33210603
- Aging 12:25020-250342020TRIB3 facilitates glioblastoma progression via restraining autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhanbin Tang et. al.PubMed 33203798
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRIB3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96RU7
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Reactivity data
Select an application| WB | ICC/IF | IHC-P | |
|---|---|---|---|
| Human | Tested | Tested | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
Associated Products
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Target data
Function
Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed:15775988, PubMed:15781252). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed:15775988, PubMed:15781252). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed:15775988, PubMed:15781252). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR: while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed:12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed:15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed:22977230).
Alternative names
C20orf97, NIPK, SKIP3, TRB3, TRIB3, Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B
Recommended products
Rabbit Polyclonal TRIB3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIB3 aa 150 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRIB3 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96RU7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIB3 also known as TRB3 or NIPK is a pseudokinase with a molecular mass of approximately 42 kDa. This protein does not possess catalytic activity. It functions largely through interactions with other proteins. TRIB3 is expressed in various tissues with high levels in the liver kidney and brain. The regulatory functions of TRIB3 involve modulating cellular processes such as stress response and apoptosis by interacting with signaling proteins.
- Biological function summary
The modulation of metabolic pathways and stress responses involves TRIB3 acting in several mechanisms. While TRIB3 itself is not part of a complex it exerts its effects by binding to and inhibiting the activity of other proteins notably AKT1 and several transcription factors. This modulation influences glucose metabolism and cellular response to unfolded protein stress indicating TRIB3's role as a significant regulator of cellular homeostasis.
- Pathways
The influence of TRIB3 on insulin signaling and the unfolded protein response is significant. Within the insulin signaling pathway TRIB3 interacts with AKT1 a central protein that regulates glucose uptake and cell survival. This interaction inhibits AKT1's activity impacting glucose metabolism. In the unfolded protein response pathway TRIB3 impacts stress response regulation through interactions that modulate transcriptional activity further highlighting its regulatory roles.
- Associated diseases and disorders
Alterations in TRIB3 expression and activity relate to conditions such as type 2 diabetes and cancer. In type 2 diabetes increased TRIB3 levels impair insulin signaling via AKT1 inhibition contributing to insulin resistance. In cancer the dysregulation of TRIB3 can promote survival pathways circumventing apoptosis and aiding in tumor progression. The involvement of TRIB3 in these diseases highlights its potential impact in therapeutic targeting.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Western blot - Anti-TRIB3 antibody (ab137526)
10% SDS PAGE
All lanes: Western blot - Anti-TRIB3 antibody (ab137526) at 1/1000 dilution
All lanes: HepG2 whole cell lysate at 30 µg
Predicted band size: 40 kDa
Western blot - Anti-TRIB3 antibody (ab137526)
All lanes: Western blot - Anti-TRIB3 antibody (ab137526) at 1/5000 dilution
All lanes: HUVEC whole cell lysate at 15 µg
Predicted band size: 40 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TRIB3 antibody (ab137526)
Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling TRIB3 with ab137526 at 1/200. The lower panel shows costaining with Hoechst 33342.
Western blot - Anti-TRIB3 antibody (ab137526)
False colour image of Western blot: Anti-TRIB3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137526 was shown to bind specifically to TRIB3. A band was observed at 40 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in TRIB3 knockout cell line Human TRIB3 knockout HCT116 cell line ab273718 (knockout cell lysate Human TRIB3 knockout HCT116 cell lysate ab275249). To generate this image, wild-type and TRIB3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-TRIB3 antibody (ab137526) at 1/1000 dilution
Lane 1: Wild-type HCT 116 cell lysate at 20 µg
Lane 2: Western blot - Human TRIB3 knockout HCT116 cell lysate (Human TRIB3 knockout HCT116 cell lysate ab275249) at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 40 kDa
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