Anti-TRIB3 antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIB3 antibody (AB137526)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells labelling TRIB3 with ab137526 at 1/200. The lower panel shows costaining with Hoechst 33342.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIB3 antibody (AB137526)

Immunohistochemical analysis of ncin87 xenograft tissue stained for TRIB3 with ab137526 at a 1/100 dilution.

Antigen retrieval : EDTA based, pH 8.0 buffer, 15min.

• WB

Lab

Western blot - Anti-TRIB3 antibody (AB137526)

False colour image of Western blot : Anti-TRIB3 antibody staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab137526 was shown to bind specifically to TRIB3. A band was observed at 40 kDa in wild-type HCT 116 cell lysates with no signal observed at this size in TRIB3 knockout cell line ab273718 (knockout cell lysate ab275249). To generate this image, wild-type and TRIB3 knockout HCT 116 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-TRIB3 antibody (ab137526) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 cell lysate at 20 µg

Lane 2:

Western blot - Human TRIB3 knockout HCT116 cell lysate (<a href='/en-us/products/cell-lysates/human-trib3-knockout-hct116-cell-lysate-ab275249'>ab275249</a>) at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

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• WB

Unknown

Western blot - Anti-TRIB3 antibody (AB137526)

All lanes:

Western blot - Anti-TRIB3 antibody (ab137526) at 1/5000 dilution

All lanes:

HUVEC whole cell lysate at 15 µg

Predicted band size: 40 kDa

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• WB

Supplier Data

Western blot - Anti-TRIB3 antibody (AB137526)

Samples were separated by 10% SDS-PAGE. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-TRIB3 antibody (ab137526) at 1/1000 dilution

Lane 1:

HeLa whole cell extracts at 30 µg

Lane 2:

HepG2 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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