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AB73547

Anti-TRIB3 antibody

2

(3 Reviews)

|

(5 Publications)

Rabbit Polyclonal TRIB3 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human TRIB3.

View Alternative Names

C20orf97, NIPK, SKIP3, TRB3, TRIB3, Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B

1 Images
Western blot - Anti-TRIB3 antibody (AB73547)
  • WB

Unknown

Western blot - Anti-TRIB3 antibody (AB73547)

All lanes:

Western blot - Anti-TRIB3 antibody (ab73547) at 1/500 dilution

All lanes:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 40 kDa

Observed band size: 38 kDa,52 kDa,60 kDa

true

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TRIB3. The exact immunogen used to generate this antibody is proprietary information.

Q96RU7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIB3 also known as TRB3 or NIPK is a pseudokinase with a molecular mass of approximately 42 kDa. This protein does not possess catalytic activity. It functions largely through interactions with other proteins. TRIB3 is expressed in various tissues with high levels in the liver kidney and brain. The regulatory functions of TRIB3 involve modulating cellular processes such as stress response and apoptosis by interacting with signaling proteins.
Biological function summary

The modulation of metabolic pathways and stress responses involves TRIB3 acting in several mechanisms. While TRIB3 itself is not part of a complex it exerts its effects by binding to and inhibiting the activity of other proteins notably AKT1 and several transcription factors. This modulation influences glucose metabolism and cellular response to unfolded protein stress indicating TRIB3's role as a significant regulator of cellular homeostasis.

Pathways

The influence of TRIB3 on insulin signaling and the unfolded protein response is significant. Within the insulin signaling pathway TRIB3 interacts with AKT1 a central protein that regulates glucose uptake and cell survival. This interaction inhibits AKT1's activity impacting glucose metabolism. In the unfolded protein response pathway TRIB3 impacts stress response regulation through interactions that modulate transcriptional activity further highlighting its regulatory roles.

Alterations in TRIB3 expression and activity relate to conditions such as type 2 diabetes and cancer. In type 2 diabetes increased TRIB3 levels impair insulin signaling via AKT1 inhibition contributing to insulin resistance. In cancer the dysregulation of TRIB3 can promote survival pathways circumventing apoptosis and aiding in tumor progression. The involvement of TRIB3 in these diseases highlights its potential impact in therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed : 15775988, PubMed : 15781252). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed : 15775988, PubMed : 15781252). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed : 15775988, PubMed : 15781252). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR : while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed : 12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed : 15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed : 22977230).
See full target information TRIB3
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell death and differentiation 29:709-721 PubMed34621049

2021

Negative feedback of SNRK to circ-SNRK regulates cardiac function post-myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Yan Wang,Xiao-Xiao Liu,Yun-Fei Deng

Molecular medicine reports 21:615-622 PubMed31974597

2020

Effect of the alteration of Tribbles homologue 3 expression on epithelial‑mesenchymal transition of transforming growth factor β1‑induced mouse alveolar epithelial cells through the Wnt/β‑catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wencheng Yu,Liyun Mi,Feifei Wang

American journal of translational research 11:5487-5500 PubMed31632524

2019

Mechanism of pomegranate ellagic polyphenols reducing insulin resistance on gestational diabetes mellitus rats.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Lan Sun,Fen-Mei Zhou,Hai-Rong Wang

Autophagy 14:2007-2022 PubMed30025493

2018

Inhibition of glioma growth by flavokawain B is mediated through endoplasmic reticulum stress induced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiwei Wang,Qichao Qi,Wenjing Zhou,Zichao Feng,Bin Huang,Anjing Chen,Di Zhang,Wenjie Li,Qing Zhang,Zheng Jiang,Rolf Bjerkvig,Lars Prestegarden,Frits Thorsen,Xinyu Wang,Xingang Li,Jian Wang

Oncogene 27:4532-43 PubMed18408768

2008

Multiple pathways are involved in the anoxia response of SKIP3 including HuR-regulated RNA stability, NF-kappaB and ATF4.

Applications

WB

Species

Human

T Rzymski,A Paantjens,J Bod,A L Harris
View all publications
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