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AB75774

Anti-TRIB3 antibody [EPR3150]

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(1 Publication)

Rabbit Recombinant Monoclonal TRIB3 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

C20orf97, NIPK, SKIP3, TRB3, TRIB3, Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B

2 Images
Western blot - Anti-TRIB3 antibody [EPR3150] (AB75774)
  • WB

Lab

Western blot - Anti-TRIB3 antibody [EPR3150] (AB75774)

False colour image of Western blot : Anti-TRIB3 antibody [EPR3150] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab75774 was shown to bind specifically to TRIB3. A band was observed at 40 kDa in wild-type HeLa cell lysates with no signal observed at this size in TRIB3 knockout cell line ab264792 (knockout cell lysate ab257765). To generate this image, wild-type and TRIB3 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-TRIB3 antibody [EPR3150] (ab75774) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

TRIB3 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human TRIB3 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-trib3-knockout-hela-cell-line-ab264792'>ab264792</a>)

Predicted band size: 40 kDa

Observed band size: 40 kDa

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Western blot - Anti-TRIB3 antibody [EPR3150] (AB75774)
  • WB

Unknown

Western blot - Anti-TRIB3 antibody [EPR3150] (AB75774)

All lanes:

Western blot - Anti-TRIB3 antibody [EPR3150] (ab75774) at 1/1000 dilution

Lane 1:

293T lysates, treated with Ionomycin and TPA at 10 µg

Lane 2:

293T lysates, untreated at 10 µg

Secondary

All lanes:

goat anti-rabbit HRP at 1/1000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

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  • Carrier free

    Anti-TRIB3 antibody [EPR3150] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3150

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIB3 also known as TRB3 or NIPK is a pseudokinase with a molecular mass of approximately 42 kDa. This protein does not possess catalytic activity. It functions largely through interactions with other proteins. TRIB3 is expressed in various tissues with high levels in the liver kidney and brain. The regulatory functions of TRIB3 involve modulating cellular processes such as stress response and apoptosis by interacting with signaling proteins.
Biological function summary

The modulation of metabolic pathways and stress responses involves TRIB3 acting in several mechanisms. While TRIB3 itself is not part of a complex it exerts its effects by binding to and inhibiting the activity of other proteins notably AKT1 and several transcription factors. This modulation influences glucose metabolism and cellular response to unfolded protein stress indicating TRIB3's role as a significant regulator of cellular homeostasis.

Pathways

The influence of TRIB3 on insulin signaling and the unfolded protein response is significant. Within the insulin signaling pathway TRIB3 interacts with AKT1 a central protein that regulates glucose uptake and cell survival. This interaction inhibits AKT1's activity impacting glucose metabolism. In the unfolded protein response pathway TRIB3 impacts stress response regulation through interactions that modulate transcriptional activity further highlighting its regulatory roles.

Alterations in TRIB3 expression and activity relate to conditions such as type 2 diabetes and cancer. In type 2 diabetes increased TRIB3 levels impair insulin signaling via AKT1 inhibition contributing to insulin resistance. In cancer the dysregulation of TRIB3 can promote survival pathways circumventing apoptosis and aiding in tumor progression. The involvement of TRIB3 in these diseases highlights its potential impact in therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed : 15775988, PubMed : 15781252). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed : 15775988, PubMed : 15781252). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed : 15775988, PubMed : 15781252). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR : while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed : 12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed : 15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed : 22977230).
See full target information TRIB3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 22: PubMed33573274

2021

Peptidylarginine Deiminase Inhibitor Application, Using Cl-Amidine, PAD2, PAD3 and PAD4 Isozyme-Specific Inhibitors in Pancreatic Cancer Cells, Reveals Roles for PAD2 and PAD3 in Cancer Invasion and Modulation of Extracellular Vesicle Signatures.

Applications

Unspecified application

Species

Unspecified reactive species

Pinar Uysal-Onganer,Stefania D'Alessio,Maria Mortoglou,Igor Kraev,Sigrun Lange
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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