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AB75846

Anti-TRIB3 antibody [EPR3151Y]

5

(1 Review)

|

(26 Publications)

Rabbit Recombinant Monoclonal TRIB3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 26 publications.

View Alternative Names

C20orf97, NIPK, SKIP3, TRB3, TRIB3, Tribbles homolog 3, TRB-3, Neuronal cell death-inducible putative kinase, SINK, p65-interacting inhibitor of NF-kappa-B

3 Images
Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (AB75846)
  • IP

Lab

Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (AB75846)

TRIB3 was immunoprecipitated from 0.35 mg 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 μg with 75846 at 1/50 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : 293T (Human embryonic kidney epithelial cell) whole cell lysate 10 μg

Lane 2 : ab75846 IP in 293T whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab75846 in 293T whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-TRIB3 antibody [EPR3151Y] (ab75846)

Predicted band size: 40 kDa

Observed band size: 40 kDa

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Western blot - Anti-TRIB3 antibody [EPR3151Y] (AB75846)
  • WB

Lab

Western blot - Anti-TRIB3 antibody [EPR3151Y] (AB75846)

Lanes 1 - 4 : Merged signal (red and green). Green - ab75846 observed at 40 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.

ab75846 was shown to react with TRIB3 in wild-type HCT 116 cells in western blot with loss of signal observed in TRIB3 knockout cell line ab273718 (TRIB3 knockout cell lysate ab275249). Wild-type and TRIB3 knockout HCT 116 cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab75846 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 10000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution

Lane 1:

Wild-type HCT116 cell lysate at 20 µg

Lane 2:

TRIB3 knockout HCT116 cell lysate at 20 µg

Lane 2:

Western blot - Human TRIB3 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-trib3-knockout-hct116-cell-line-ab273718'>ab273718</a>)

Lane 3:

Hap1 cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

Western blot - Anti-TRIB3 antibody [EPR3151Y] (AB75846)
  • WB

Unknown

Western blot - Anti-TRIB3 antibody [EPR3151Y] (AB75846)

All lanes:

Western blot - Anti-TRIB3 antibody [EPR3151Y] (ab75846) at 1/10000 dilution

Lane 1:

293T cell lysates, treated with Ionomycin and TPA at 10 µg

Lane 2:

293T cell lysates, untreated at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 40 kDa

Observed band size: 40 kDa

false

  • Carrier free

    Anti-TRIB3 antibody [EPR3151Y] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3151Y

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000 - 1/20000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIB3 also known as TRB3 or NIPK is a pseudokinase with a molecular mass of approximately 42 kDa. This protein does not possess catalytic activity. It functions largely through interactions with other proteins. TRIB3 is expressed in various tissues with high levels in the liver kidney and brain. The regulatory functions of TRIB3 involve modulating cellular processes such as stress response and apoptosis by interacting with signaling proteins.
Biological function summary

The modulation of metabolic pathways and stress responses involves TRIB3 acting in several mechanisms. While TRIB3 itself is not part of a complex it exerts its effects by binding to and inhibiting the activity of other proteins notably AKT1 and several transcription factors. This modulation influences glucose metabolism and cellular response to unfolded protein stress indicating TRIB3's role as a significant regulator of cellular homeostasis.

Pathways

The influence of TRIB3 on insulin signaling and the unfolded protein response is significant. Within the insulin signaling pathway TRIB3 interacts with AKT1 a central protein that regulates glucose uptake and cell survival. This interaction inhibits AKT1's activity impacting glucose metabolism. In the unfolded protein response pathway TRIB3 impacts stress response regulation through interactions that modulate transcriptional activity further highlighting its regulatory roles.

Alterations in TRIB3 expression and activity relate to conditions such as type 2 diabetes and cancer. In type 2 diabetes increased TRIB3 levels impair insulin signaling via AKT1 inhibition contributing to insulin resistance. In cancer the dysregulation of TRIB3 can promote survival pathways circumventing apoptosis and aiding in tumor progression. The involvement of TRIB3 in these diseases highlights its potential impact in therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Inactive protein kinase which acts as a regulator of the integrated stress response (ISR), a process for adaptation to various stress (PubMed : 15775988, PubMed : 15781252). Inhibits the transcriptional activity of DDIT3/CHOP and is involved in DDIT3/CHOP-dependent cell death during ER stress (PubMed : 15775988, PubMed : 15781252). May play a role in programmed neuronal cell death but does not appear to affect non-neuronal cells (PubMed : 15775988, PubMed : 15781252). Acts as a negative feedback regulator of the ATF4-dependent transcription during the ISR : while TRIB3 expression is promoted by ATF4, TRIB3 protein interacts with ATF4 and inhibits ATF4 transcription activity (By similarity). Disrupts insulin signaling by binding directly to Akt kinases and blocking their activation (By similarity). May bind directly to and mask the 'Thr-308' phosphorylation site in AKT1 (By similarity). Interacts with the NF-kappa-B transactivator p65 RELA and inhibits its phosphorylation and thus its transcriptional activation activity (PubMed : 12736262). Interacts with MAPK kinases and regulates activation of MAP kinases (PubMed : 15299019). Can inhibit APOBEC3A editing of nuclear DNA (PubMed : 22977230).
See full target information TRIB3
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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 36:431-441 PubMed39910904

2025

Tanshinone IIA Restrains Hepatocellular Carcinoma Progression by Regulating METTL3-Mediated m6A Modification of TRIB3 mRNA.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Jiang,Xinjie Wang,Zhenyang Wang,Shengliang Zhang,Jianhua Wang,Xianglong Wang,Yang Zhang

Frontiers in pharmacology 15:1517708 PubMed39881875

2025

Parishin B blocking TRIB3-AKT1 interaction inhibits breast cancer lung metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiongtao Cheng,Jianguo Sun,Shouhong Chen,Nan Wang,Weijing Tang,Zijian Xia,Yuhong Shu,Shouhong Gao,Zhipeng Wang,Xinxia Wang,Rongzi Shao,Jianxiong Cao

Frontiers in microbiology 15:1456108 PubMed39211324

2024

FGF21 upregulation by hepatitis C virus via the eIF2α-ATF4 pathway: implications for interferon signaling suppression and TRIM31-mediated TSC degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Liu,Masahiko Ito,Satoshi Sakai,Jie Liu,Kazuyoshi Ohta,Kenji Saito,Kenji Nakashima,Shinya Satoh,Alu Konno,Tetsuro Suzuki

Cancer medicine 13:e7456 PubMed39118481

2024

A multivariate retrospective analysis of high-grade gliomas: Survival and prognostic factors.

Applications

Unspecified application

Species

Unspecified reactive species

Weiyan Shi,Xuanzhong Wang,Shiyu Liu,Zhuangzhuang Zheng,Lihua Dong,Xin Jiang

Cell death & disease 15:178 PubMed38429254

2024

TRIB3 promotes malignancy of head and neck squamous cell carcinoma via inhibiting ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Chen,Wanzun Lin,Haojiong Zhang,Shikai Geng,Ziyu Le,Fangzhu Wan,Qingting Huang,Huaiyuan Chen,Xingyu Liu,Jiade J Lu,Lin Kong

Frontiers in immunology 14:1324205 PubMed38090559

2023

Lipid metabolism-related gene expression in the immune microenvironment predicts prognostic outcomes in renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Zhang,Bingbiao Lin,Huikun Chen,Yinyan Ye,Yijie Huang,Zhen Chen,Jun Li

Kidney & blood pressure research 48:611-627 PubMed37717559

2023

Transcription Factor Sox9 Exacerbates Kidney Injury through Inhibition of MicroRNA-96-5p and Activation of the Trib3/IL-6 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Wang,Guang Chen,Yongqiang Du,Jiajia Yang,Wei Wang

eLife 11: PubMed36107759

2022

GCN2 eIF2 kinase promotes prostate cancer by maintaining amino acid homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Ricardo A Cordova,Jagannath Misra,Parth H Amin,Anglea J Klunk,Nur P Damayanti,Kenneth R Carlson,Andrew J Elmendorf,Hyeong-Geug Kim,Emily T Mirek,Bennet D Elzey,Marcus J Miller,X Charlie Dong,Liang Cheng,Tracy G Anthony,Roberto Pili,Ronald C Wek,Kirk A Staschke

Bioengineered 13:10386-10398 PubMed35465829

2022

Tribbles homolog 3 contributes to high glucose-induced injury in retinal pigment epithelial cells via binding to growth factor receptor-bound 2.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Liao,Xuefeng Gao

BMC cancer 22:459 PubMed35473511

2022

Deletion of TRIB3 disrupts the tumor progression induced by integrin αvβ3 in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Junjun Ma,Lifeng Meng,Dabei Liu,Jun Chen
View all publications
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Product promise

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