AB58755

Anti-Trichohyalin antibody [AE15]

Name: Anti-Trichohyalin antibody [AE15] (ab58755) | Abcam
Brand: Abcam Limited
SKU: AB58755
Price: 510 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(17 Publications)

Mouse Monoclonal Trichohyalin antibody. Suitable for WB, IHC-P, IHC-Fr and reacts with Human, Mouse samples. Cited in 17 publications. Immunogen corresponding to Tissue preparation containing TCHH protein.

View Alternative Names

THH, THL, TRHY, TCHH, Trichohyalin

1 Images

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Western blot - Anti-Trichohyalin antibody [AE15] (AB58755)

All lanes:

Western blot - Anti-Trichohyalin antibody [AE15] (ab58755) at 1 µg/mL

All lanes:

Lung (Human) Tissue Lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 254 kDa

Observed band size: 165 kDa,220 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AE15

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P, IHC-Fr

applications

Immunogen

Tissue preparation containing TCHH protein. The exact immunogen used to generate this antibody is proprietary information.

Q07283

Specificity

This antibody recognises a 220K trichohyalin granular protein, a marker for hair follicular differentiation.

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Trichohyalin (THH) acts mechanically as a structural protein that plays an important role in hair and skin cell cornification. Expressed in the inner root sheath of hair follicles and in the granular layer of the epidermis it contributes to cell envelope formation. This protein with a molecular mass of approximately 250 kDa is also known as trichohyalin-1. THH is rich in glutamine and lysine which allows it to bind to other proteins and form a stable matrix structure in hair and skin.

Biological function summary

Trichohyalin contributes to the development and maintenance of hair follicle integrity and skin barrier function. As part of the keratinocyte differentiation process it interacts with various keratins and contributes to cell envelope formation by reinforcing cell membranes through cross-linking. The protein forms a part of the cornified cell envelope complex where it binds with filaggrin and loricrin aiding in cellular structural support and resistance to mechanical stress.

Pathways

Trichohyalin integrates into the cellular pathways involved in keratinocyte differentiation and cornification. It participates closely in the epidermal differentiation complex a critical pathway for skin barrier function. Trichohyalin works alongside proteins such as involucrin and filaggrin to facilitate these processes ensuring the maintenance of skin homeostasis and protective barrier formation.

The altered expression or malfunction of trichohyalin links to disorders like ichthyosis vulgaris and hair shaft abnormalities. These conditions involve aberrations in the epidermal differentiation pathway. Mutations in trichohyalin can affect hair texture and fragility often seen in conjunction with other keratin-related abnormalities. Researchers observe that defective trichohyalin also relates to structural skin anomalies associated with the malfunction of proteins like loricrin and involucrin.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.

See full target information TCHH

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Publications (17)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 32:2160-2165 PubMed36995036

2023

Altered expression of S100 fused-type proteins in an atopic dermatitis skin model.

Applications

Unspecified application

Species

Unspecified reactive species

Teruhiko Makino,Megumi Mizawa,Keita Takemoto,Seiji Yamamoto,Tadamichi Shimizu

Dermatology (Basel, Switzerland) 239:60-71 PubMed35843211

2022

Effects of NCSTN Mutation on Hair Follicle Components in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Wei Shi,Wei Cao,Qing-Zan Zhao,Hong-Xing Yu,Si-Sen Zhang,Yi-Bin Hao

Molecular biology of the cell 29:2784-2799 PubMed30188763

2018

Suppression of Wnt/β-catenin signaling by EGF receptor is required for hair follicle development.

Applications

Unspecified application

Species

Unspecified reactive species

Swamy K Tripurani,Yan Wang,Ying-Xin Fan,Massod Rahimi,Lily Wong,Min-Hyung Lee,Matthew F Starost,Jeffrey S Rubin,Gibbes R Johnson

Experimental dermatology 29:349-356 PubMed30033549

2018

Dissociated skin cells regenerate hair follicles in a microwound, "The Punch Assay".

Applications

Unspecified application

Species

Unspecified reactive species

Xue Leng,Ping Wang,Zhihong Chen,Dingyang Li,Jie Wen,Xuan Zhang,Hua Qian,Jing Guo,Xunwei Wu

The Journal of investigative dermatology 138:500-510 PubMed28964716

2017

Delayed Hair Follicle Morphogenesis and Hair Follicle Dystrophy in a Lipoatrophy Mouse Model of Pparg Total Deletion.

Applications

Unspecified application

Species

Unspecified reactive species

Chiara Sardella,Carine Winkler,Laure Quignodon,Jonathan A Hardman,Barbara Toffoli,Greta Maria Paola Giordano Attianese,Jennifer E Hundt,Liliane Michalik,Charles R Vinson,Ralf Paus,Béatrice Desvergne,Federica Gilardi

Journal of toxicologic pathology 30:79-107 PubMed28190929

2017

Databases for technical aspects of immunohistochemistry.

Applications

IHC

Species

Rabbit

Satoshi Furukawa,Mika Nagaike,Kiyokazu Ozaki

American journal of human genetics 98:1030-1037 PubMed27153399

2016

Somatic Mutations in NEK9 Cause Nevus Comedonicus.

Applications

Unspecified application

Species

Unspecified reactive species

Jonathan L Levinsohn,Jeffrey L Sugarman,Jennifer M McNiff,Richard J Antaya,Keith A Choate

Experimental dermatology 25:355-61 PubMed26739701

2016

ABCG2 deficiency in skin impairs re-epithelialization in cutaneous wound healing.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiao-Min Chang,Wen-Yen Huang,Sung-Jan Lin,Wei-Chao Huang,Chia-Rui Shen,Wan-Yu Mao,Chia-Ning Shen

The American journal of pathology 186:1036-43 PubMed26873447

2016

Activation of the OVOL1-OVOL2 Axis in the Hair Bulb and in Pilomatricoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takamichi Ito,Gaku Tsuji,Fumitaka Ohno,Hiroshi Uchi,Takeshi Nakahara,Akiko Hashimoto-Hachiya,Yuichi Yoshida,Osamu Yamamoto,Yoshinao Oda,Masutaka Furue

Nature genetics 48:152-8 PubMed26691985

2015

Regulatory mutations in TBX3 disrupt asymmetric hair pigmentation that underlies Dun camouflage color in horses.

Applications

Unspecified application

Species

Unspecified reactive species

Freyja Imsland,Kelly McGowan,Carl-Johan Rubin,Corneliu Henegar,Elisabeth Sundström,Jonas Berglund,Doreen Schwochow,Ulla Gustafson,Páll Imsland,Kerstin Lindblad-Toh,Gabriella Lindgren,Sofia Mikko,Lee Millon,Claire Wade,Mikkel Schubert,Ludovic Orlando,Maria Cecilia T Penedo,Gregory S Barsh,Leif Andersson

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Trichohyalin antibody [AE15]

Western blot - Anti-Trichohyalin antibody [AE15] (AB58755)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (17)

Product promise