Mouse Recombinant Monoclonal Trichohyalin antibody. Carrier free. Suitable for IHC-P and reacts with Rat samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trichohyalin antibody [AE15] - BSA and Azide free (AB255965), expandable thumbnail](https://content.abcam.com/products/images/trichohyalin-antibody-ae15-bsa-and-azide-free-ab255965--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12700.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trichohyalin antibody [AE15] - BSA and Azide free (AB255965)")
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC-Fr | IHC-P | |
|---|---|---|---|
| Rat | Not recommended | Not recommended | Tested |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/2000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Intermediate filament-associated protein that associates in regular arrays with keratin intermediate filaments (KIF) of the inner root sheath cells of the hair follicle and the granular layer of the epidermis. It later becomes cross-linked to KIF by isodipeptide bonds. It may serve as scaffold protein, together with involucrin, in the organization of the cell envelope or even anchor the cell envelope to the KIF network. It may be involved in its own calcium-dependent postsynthetic processing during terminal differentiation.
Alternative names
THH, THL, TRHY, TCHH, Trichohyalin
Recommended products
Mouse Recombinant Monoclonal Trichohyalin antibody. Carrier free. Suitable for IHC-P and reacts with Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- AE15
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255965 is the carrier-free version of Anti-Trichohyalin antibody [AE15] ab58755.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Trichohyalin (THH) acts mechanically as a structural protein that plays an important role in hair and skin cell cornification. Expressed in the inner root sheath of hair follicles and in the granular layer of the epidermis it contributes to cell envelope formation. This protein with a molecular mass of approximately 250 kDa is also known as trichohyalin-1. THH is rich in glutamine and lysine which allows it to bind to other proteins and form a stable matrix structure in hair and skin.
- Biological function summary
Trichohyalin contributes to the development and maintenance of hair follicle integrity and skin barrier function. As part of the keratinocyte differentiation process it interacts with various keratins and contributes to cell envelope formation by reinforcing cell membranes through cross-linking. The protein forms a part of the cornified cell envelope complex where it binds with filaggrin and loricrin aiding in cellular structural support and resistance to mechanical stress.
- Pathways
Trichohyalin integrates into the cellular pathways involved in keratinocyte differentiation and cornification. It participates closely in the epidermal differentiation complex a critical pathway for skin barrier function. Trichohyalin works alongside proteins such as involucrin and filaggrin to facilitate these processes ensuring the maintenance of skin homeostasis and protective barrier formation.
- Associated diseases and disorders
The altered expression or malfunction of trichohyalin links to disorders like ichthyosis vulgaris and hair shaft abnormalities. These conditions involve aberrations in the epidermal differentiation pathway. Mutations in trichohyalin can affect hair texture and fragility often seen in conjunction with other keratin-related abnormalities. Researchers observe that defective trichohyalin also relates to structural skin anomalies associated with the malfunction of proteins like loricrin and involucrin.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trichohyalin antibody [AE15] - BSA and Azide free (ab255965), expandable thumbnail](https://content.abcam.com/products/images/trichohyalin-antibody-ae15-bsa-and-azide-free-ab255965--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img12700.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trichohyalin antibody [AE15] - BSA and Azide free (ab255965)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Trichohyalin antibody [AE15] - BSA and Azide free (ab255965)
Immunohistochemical analysis of paraffin-embedded Rat skin tissue labeling Trichohyalin1 with Anti-Trichohyalin antibody [AE15] ab58755 at 0.241μg/ml followed by a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879). Positive staining on the rat skin is observed. The section was incubated with Anti-Trichohyalin antibody [AE15] ab58755 for 30 mins at room temperature.The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Mouse IgG H&L (HRP polymer) (Goat Anti-Mouse IgG H&L (HRP polymer) ab214879).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Trichohyalin antibody [AE15] ab58755).
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