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AB151306

Anti-TRIM10 antibody

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(3 Publications)

Rabbit Polyclonal TRIM10 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM10 aa 1-300.

View Alternative Names

RFB30, RNF9, TRIM10, Tripartite motif-containing protein 10, B30-RING finger protein, RING finger protein 9

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TRIM10 antibody (AB151306)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIM10 antibody (AB151306)

Immunofluorescent analysis of methanol-fixed MCF7 cells labeling TRIM10 with ab151306 antibody at 1/200 dilution. Bottom figure represents cells co-stained with Hoechst 33342.

Western blot - Anti-TRIM10 antibody (AB151306)
  • WB

Unknown

Western blot - Anti-TRIM10 antibody (AB151306)

10% SDS PAGE

All lanes:

Western blot - Anti-TRIM10 antibody (ab151306) at 1/1000 dilution

All lanes:

NT2D1 whole cells lysate at 30 µg

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TRIM10 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9UDY6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM10 also known as Tripartite Motif Containing 10 is a protein that belongs to the TRIM family characterized by its tripartite motif which includes a RING finger one or two B-box domains and a coiled-coil region. It has an approximate molecular mass of 60 kDa. TRIM10 expresses mainly in hematopoietic tissues such as the bone marrow and various cell types within the immune system. Its structure suggests a role in protein-protein interactions particularly those involved with ubiquitination processes.
Biological function summary

TRIM10 participates in immune response modulation and iron homeostasis. Within biological systems it seems to play a part in a protein complex that influences the cytoskeleton's organization and cellular trafficking. TRIM10 supports erythropoiesis facilitating the maturation and differentiation of erythroid cells in the bone marrow. This involvement highlights its influence in maintaining effective functional condition of red blood cell populations.

Pathways

TRIM10 participates in the ubiquitin-proteasome pathway which controls the degradation of proteins and is important for regulating protein levels and function in cells. It engages particularly within the heme biosynthesis pathway tying it to proteins like ferrochelatase and other enzymes necessary for the iron incorporation into heme. These pathways are essential for managing both cellular iron levels and oxidative stress responses.

TRIM10 has associations with conditions like sickle cell disease and anemia. Dysregulation or mutation of TRIM10 can lead to impaired erythroid differentiation or function contributing to these hematologic disorders. Its connection to proteins involved in iron metabolism means that any irregularities may echo into broader heme production issues linking it further to hemolytic diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ligase that plays an essential role in the differentiation and survival of terminal erythroid cells. May directly bind to PTEN and promote its ubiquitination, resulting in its proteasomal degradation and activation of hypertrophic signaling (By similarity). In addition, plays a role in immune response regulation by repressing the phosphorylation of STAT1 and STAT2 in the interferon/JAK/STAT signaling pathway independent of its E3 ligase activity. Mechanistically, interacts with the intracellular domain of IFNAR1 and thereby inhibits the association between TYK2 and IFNAR1 (PubMed : 33811647).
See full target information TRIM10
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancers 15: PubMed36672365

2023

TRIM10 Is Downregulated in Acute Myeloid Leukemia and Plays a Tumor Suppressive Role via Regulating NF-κB Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Li,Qi Li,Zhengrong Zou,Zoufang Huang,Yijian Chen

Journal of cellular and molecular medicine 24:6233-6241 PubMed32343488

2020

Tripartite motif 10 regulates cardiac hypertrophy by targeting the PTEN/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Yang,Xiao-Xiao Wang,Chun-Yu Zhou,Xue Xiao,Cui Tian,Hui-Hua Li,Chun-Lin Yin,Hong-Xia Wang

The EMBO journal 36:42-60 PubMed27932448

2016

Dedicated SNAREs and specialized TRIM cargo receptors mediate secretory autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Tomonori Kimura,Jingyue Jia,Suresh Kumar,Seong Won Choi,Yuexi Gu,Michal Mudd,Nicolas Dupont,Shanya Jiang,Ryan Peters,Farzin Farzam,Ashish Jain,Keith A Lidke,Christopher M Adams,Terje Johansen,Vojo Deretic
View all publications
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