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AB194477

Anti-TRIM13 antibody - N-terminal

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(2 Publications)

Mouse Polyclonal TRIM13 antibody. N-terminal. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM13 aa 1-100.

View Alternative Names

LEU5, RFP2, RNF77, TRIM13, E3 ubiquitin-protein ligase TRIM13, B-cell chronic lymphocytic leukemia tumor suppressor Leu5, Leukemia-associated protein 5, Putative tumor suppressor RFP2, RING finger protein 77, RING-type E3 ubiquitin transferase TRIM13, Ret finger protein 2, Tripartite motif-containing protein 13

2 Images
Western blot - Anti-TRIM13 antibody - N-terminal (AB194477)
  • WB

Supplier Data

Western blot - Anti-TRIM13 antibody - N-terminal (AB194477)

All lanes:

Western blot - Anti-TRIM13 antibody - N-terminal (ab194477) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 47 kDa

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Western blot - Anti-TRIM13 antibody - N-terminal (AB194477)
  • WB

Supplier Data

Western blot - Anti-TRIM13 antibody - N-terminal (AB194477)

Predicted MWt of immunogen : 37.11 KDa.

All lanes:

Western blot - Anti-TRIM13 antibody - N-terminal (ab194477) at 1/1000 dilution

All lanes:

recombinant immunogen at 0.2 µg

Predicted band size: 47 kDa

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Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human TRIM13 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

O60858

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Constituents: 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM13 also known as RFP2 is a protein encoded by the TRIM13 gene with an approximate mass of 42 kDa. Mechanically TRIM13 functions as an E3 ubiquitin ligase mediating ubiquitination and proteasomal degradation. It belongs to the tripartite motif family characterized by a RING zinc finger one or more B-box domains and a coiled-coil region. The protein is expressed in various tissues including the heart liver and pancreas suggesting a wide range of functional roles.
Biological function summary

This protein is important for maintaining cellular homeostasis by regulating protein turnover and quality control. TRIM13 is involved in the endoplasmic reticulum-associated degradation (ERAD) pathway where it facilitates the removal of misfolded proteins. Additionally it interacts with other molecules to form complexes that modulate autophagy a process vital for cellular cleaning and response to stress.

Pathways

Ubiquitination processes and quality control systems incorporate TRIM13 into the intricacies of the ERAD and autophagy pathways. It coordinates with proteins like p62 and Beclin1 which are important for autophagy regulation. In the ERAD pathway TRIM13 functions alongside components such as Derlin-1 helping to identify and direct aberrant proteins toward degradation. These interactions balance protein synthesis and degradation essential for cellular stress responses and survival.

TRIM13's roles tie to neurodegenerative diseases and cancer. Mutations or dysregulation of TRIM13 lead to an accumulation of misfolded proteins contributing to the progression of amyotrophic lateral sclerosis (ALS). In cancer altered TRIM13 expression can disrupt normal degradation processes affecting tumor growth and metastasis. TRIM13 interacts with CHOP a protein involved in pro-apoptotic signaling which links it to cellular stress responses that are pivotal in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor (PubMed : 22178386). Also plays a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation (PubMed : 28087809). Participates as well in T-cell receptor-mediated NF-kappa-B activation (PubMed : 25088585). In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B (PubMed : 25152375).
See full target information TRIM13
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113101 PubMed40746991

2025

TRIM13 prevents cardiomyocyte injury by affecting apoptosis through interacting with CD3D in myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Feipeng Wu,Xiandong Zheng,Qiyan Wu,Liju Hong,Lei Yue,Rui Yang,Dandan Chen,Youjun Zhou

Science advances 8:eabm0478 PubMed35353581

2022

eIF4A2 targets developmental potency and histone H3.3 transcripts for translational control of stem cell pluripotency.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Li,Jihong Yang,Xin Huang,Hongwei Zhou,Jianlong Wang
View all publications
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