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Mouse Polyclonal TRIM13 antibody. N-terminal. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase TRIM13 aa 1-100.

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Images

Western blot - Anti-TRIM13 antibody - N-terminal (AB194477), expandable thumbnail
  • Western blot - Anti-TRIM13 antibody - N-terminal (AB194477), expandable thumbnail

Key facts

Isotype

IgG

Host species

Mouse

Storage buffer

Constituents: 50% Glycerol (glycerin, glycerine)

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase TRIM13 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O60858

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Predicted
Rat
Predicted
Cow
Predicted
Recombinant fragment - Human
Tested

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/500 - 1/1000

Notes

-

Species

Human

Dilution info

1/500 - 1/1000

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Endoplasmic reticulum (ER) membrane anchored E3 ligase involved in the retrotranslocation and turnover of membrane and secretory proteins from the ER through a set of processes named ER-associated degradation (ERAD). This process acts on misfolded proteins as well as in the regulated degradation of correctly folded proteins. Enhances ionizing radiation-induced p53/TP53 stability and apoptosis via ubiquitinating MDM2 and AKT1 and decreasing AKT1 kinase activity through MDM2 and AKT1 proteasomal degradation. Regulates ER stress-induced autophagy, and may act as a tumor suppressor (PubMed:22178386). Also plays a role in innate immune response by stimulating NF-kappa-B activity in the TLR2 signaling pathway. Ubiquitinates TRAF6 via the 'Lys-29'-linked polyubiquitination chain resulting in NF-kappa-B activation (PubMed:28087809). Participates as well in T-cell receptor-mediated NF-kappa-B activation (PubMed:25088585). In the presence of TNF, modulates the IKK complex by regulating IKBKG/NEMO ubiquitination leading to the repression of NF-kappa-B (PubMed:25152375).

Alternative names

Recommended products

Mouse Polyclonal TRIM13 antibody. N-terminal. Suitable for WB and reacts with Recombinant fragment - Human, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase TRIM13 aa 1-100.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human E3 ubiquitin-protein ligase TRIM13 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link O60858
Purity

Whole antiserum

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRIM13 also known as RFP2 is a protein encoded by the TRIM13 gene with an approximate mass of 42 kDa. Mechanically TRIM13 functions as an E3 ubiquitin ligase mediating ubiquitination and proteasomal degradation. It belongs to the tripartite motif family characterized by a RING zinc finger one or more B-box domains and a coiled-coil region. The protein is expressed in various tissues including the heart liver and pancreas suggesting a wide range of functional roles.

Biological function summary

This protein is important for maintaining cellular homeostasis by regulating protein turnover and quality control. TRIM13 is involved in the endoplasmic reticulum-associated degradation (ERAD) pathway where it facilitates the removal of misfolded proteins. Additionally it interacts with other molecules to form complexes that modulate autophagy a process vital for cellular cleaning and response to stress.

Pathways

Ubiquitination processes and quality control systems incorporate TRIM13 into the intricacies of the ERAD and autophagy pathways. It coordinates with proteins like p62 and Beclin1 which are important for autophagy regulation. In the ERAD pathway TRIM13 functions alongside components such as Derlin-1 helping to identify and direct aberrant proteins toward degradation. These interactions balance protein synthesis and degradation essential for cellular stress responses and survival.

Associated diseases and disorders

TRIM13's roles tie to neurodegenerative diseases and cancer. Mutations or dysregulation of TRIM13 lead to an accumulation of misfolded proteins contributing to the progression of amyotrophic lateral sclerosis (ALS). In cancer altered TRIM13 expression can disrupt normal degradation processes affecting tumor growth and metastasis. TRIM13 interacts with CHOP a protein involved in pro-apoptotic signaling which links it to cellular stress responses that are pivotal in disease states.

Product promise

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2 product images

  • Western blot - Anti-TRIM13 antibody - N-terminal (ab194477), expandable thumbnail

    Western blot - Anti-TRIM13 antibody - N-terminal (ab194477)

    All lanes: Western blot - Anti-TRIM13 antibody - N-terminal (ab194477) at 1/500 dilution

    All lanes: HeLa cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa

  • Western blot - Anti-TRIM13 antibody - N-terminal (ab194477), expandable thumbnail

    Western blot - Anti-TRIM13 antibody - N-terminal (ab194477)

    Predicted MWt of immunogen: 37.11 KDa.

    All lanes: Western blot - Anti-TRIM13 antibody - N-terminal (ab194477) at 1/1000 dilution

    All lanes: recombinant immunogen at 0.2 µg

    Developed using the ECL technique.

    Predicted band size: 47 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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