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AB251749

Anti-TRIM16 antibody

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(1 Publication)

Rabbit Polyclonal TRIM16 antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM16 aa 50-250.

View Alternative Names

EBBP, TRIM16, Tripartite motif-containing protein 16, E3 ubiquitin-protein ligase TRIM16, Estrogen-responsive B box protein

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM16 antibody (AB251749)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM16 antibody (AB251749)

Paraffin-embedded human esophagus tissue stained for TRIM16 using ab251749 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM16 antibody (AB251749)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM16 antibody (AB251749)

Paraffin-embedded human cerbellum tissue stained for TRIM16 using ab251749 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM16 antibody (AB251749)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM16 antibody (AB251749)

Paraffin-embedded human stomach tissue stained for TRIM16 using ab251749 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-TRIM16 antibody (AB251749)
  • WB

Unknown

Western blot - Anti-TRIM16 antibody (AB251749)

All lanes:

Western blot - Anti-TRIM16 antibody (ab251749) at 0.4 µg/mL

All lanes:

Human cell line A-549

Predicted band size: 64 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRIM16 aa 50-250. The exact immunogen used to generate this antibody is proprietary information.

O95361

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM16 also known as Tripartite Motif Containing 16 is a protein involved in several cellular mechanisms. It has a molecular mass of approximately 64 kDa. This protein is part of the TRIM protein family characterized by the presence of a RING finger domain one or two zinc-binding motifs called B-boxes and a coiled-coil region. TRIM16 is expressed in many tissues including the skin lungs and brain. The protein exhibits E3 ubiquitin ligase activity which tags other proteins for degradation.
Biological function summary

This protein plays a role in regulating cell differentiation and proliferation. TRIM16 is not known to form large complexes but its interactions with other proteins allow it to exert its biological effects. It interacts with factors that influence cellular growth pathways contributing to the control of cellular homeostasis. Its functional roles include modulation of specific transcription factors and involvement in the regulation of inflammation.

Pathways

TRIM16 participates in the interferon signaling pathway and the Wnt/β-catenin signaling pathway. Its involvement in the interferon signaling pathway indicates a role in immune response regulation while its activity in the Wnt/β-catenin pathway suggests a function in cellular development and differentiation. TRIM16 interacts with proteins like β-catenin and STAT1 integrating signals from different pathways to maintain normal cellular functions.

Alterations in TRIM16 expression and function associate with cancer and inflammatory diseases. In cancer TRIM16 often acts as a tumor suppressor with its underexpression linked to increased tumorigenic potential. It interacts with proteins such as p53 to carry out these functions. In the context of inflammatory diseases TRIM16 influences cytokine production closely connected with inflammation regulation and interacts with proteins like NF-kB.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.
See full target information TRIM16
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pathogens (Basel, Switzerland) 12: PubMed37375542

2023

TRIM16 Overexpression in HEK293T Cells Results in Cell Line-Specific Antiviral Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Lance R Nigos,Nichollas E Scott,Andrew G Brooks,Malika Ait-Goughoulte,Sarah L Londrigan,Patrick C Reading,Rubaiyea Farrukee
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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