AB72129

Anti-TRIM16 antibody

Name: Anti-TRIM16 antibody (ab72129) | Abcam
Brand: Abcam Limited
SKU: AB72129
Price: 520 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal TRIM16 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM16.

View Alternative Names

EBBP, TRIM16, Tripartite motif-containing protein 16, E3 ubiquitin-protein ligase TRIM16, Estrogen-responsive B box protein

2 Images

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Immunoprecipitation - Anti-TRIM16 antibody (AB72129)

Immunoprecipitation/ Western Blot of TRIM16.
Lane 1 : ab72129 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab72129 at 1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-TRIM16 antibody (ab72129)

Predicted band size: 64 kDa

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Western blot - Anti-TRIM16 antibody (AB72129)

All lanes:

Western blot - Anti-TRIM16 antibody (ab72129) at 0.04 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Lane 5:

Whole cell lysate from NIH3T3 cells at 50 µg

Predicted band size: 64 kDa

Observed band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human TRIM16. The exact immunogen used to generate this antibody is proprietary information.

O95361

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM16 also known as Tripartite Motif Containing 16 is a protein involved in several cellular mechanisms. It has a molecular mass of approximately 64 kDa. This protein is part of the TRIM protein family characterized by the presence of a RING finger domain one or two zinc-binding motifs called B-boxes and a coiled-coil region. TRIM16 is expressed in many tissues including the skin lungs and brain. The protein exhibits E3 ubiquitin ligase activity which tags other proteins for degradation.

Biological function summary

This protein plays a role in regulating cell differentiation and proliferation. TRIM16 is not known to form large complexes but its interactions with other proteins allow it to exert its biological effects. It interacts with factors that influence cellular growth pathways contributing to the control of cellular homeostasis. Its functional roles include modulation of specific transcription factors and involvement in the regulation of inflammation.

Pathways

TRIM16 participates in the interferon signaling pathway and the Wnt/β-catenin signaling pathway. Its involvement in the interferon signaling pathway indicates a role in immune response regulation while its activity in the Wnt/β-catenin pathway suggests a function in cellular development and differentiation. TRIM16 interacts with proteins like β-catenin and STAT1 integrating signals from different pathways to maintain normal cellular functions.

Alterations in TRIM16 expression and function associate with cancer and inflammatory diseases. In cancer TRIM16 often acts as a tumor suppressor with its underexpression linked to increased tumorigenic potential. It interacts with proteins such as p53 to carry out these functions. In the context of inflammatory diseases TRIM16 influences cytokine production closely connected with inflammation regulation and interacts with proteins like NF-kB.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase that plays an essential role in the organization of autophagic response and ubiquitination upon lysosomal and phagosomal damages. Plays a role in the stress-induced biogenesis and degradation of protein aggresomes by regulating the p62-KEAP1-NRF2 signaling and particularly by modulating the ubiquitination levels and thus stability of NRF2. Acts as a scaffold protein and facilitates autophagic degradation of protein aggregates by interacting with p62/SQSTM, ATG16L1 and LC3B/MAP1LC3B. In turn, protects the cell against oxidative stress-induced cell death as a consequence of endomembrane damage.

See full target information TRIM16

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of diabetes investigation 15:300-314 PubMed38149724

2023

Cordyceps cicadae polysaccharides attenuate diabetic nephropathy via the miR-30a-3p/TRIM16 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Zheng,Qin Xu,Yiwen Wang,Yifei Zhong,Rong Zhu

Molecular medicine reports 28: PubMed37417356

2023

Saikosaponin A protects against uremic toxin indole‑3 acetic acid‑induced damage to the myocardium.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Chen,Xiaoyuan Hu,Xinguang Chen

Environmental toxicology 37:1957-1967 PubMed35442568

2022

CRABP2 accelerates epithelial mesenchymal transition in serous ovarian cancer cells by promoting TRIM16 methylation via upregulating EZH2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Xie,Minghua Tan,Yang Gao,Hong Yang

Bioengineered 13:3651-3664 PubMed35230201

2022

CircPTK2 inhibits cell cisplatin (CDDP) resistance by targeting miR-942/TRIM16 axis in non-small cell lung cancer (NSCLC).

Applications

Unspecified application

Species

Unspecified reactive species

Yongfu Wang,Yuanlin Wu,Shaoqiang Xie

Oncology research 26:1005-1014 PubMed29295721

2018

Downregulation of MicroRNA-135 Promotes Sensitivity of Non-Small Cell Lung Cancer to Gefitinib by Targeting TRIM16.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Wang,Tingting Zhang

PloS one 7:e44095 PubMed22952885

2012

Novel direct targets of miR-19a identified in breast cancer cells by a quantitative proteomic approach.

Applications

Unspecified application

Species

Unspecified reactive species

Mamoru Ouchida,Hirotaka Kanzaki,Sachio Ito,Hiroko Hanafusa,Yoshimi Jitsumori,Seiji Tamaru,Kenji Shimizu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM16 antibody

Immunoprecipitation - Anti-TRIM16 antibody (AB72129)

Western blot - Anti-TRIM16 antibody (AB72129)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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