AB3942

Anti-TRIM2 antibody

Name: Anti-TRIM2 antibody (ab3942) | Abcam
Brand: Abcam Limited
SKU: AB3942
Price: 520 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Goat Polyclonal TRIM2 antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM2 aa 700 to C-terminus.

View Alternative Names

KIAA0517, RNF86, TRIM2, Tripartite motif-containing protein 2, E3 ubiquitin-protein ligase TRIM2, RING finger protein 86, RING-type E3 ubiquitin transferase TRIM2

1 Images

Unknown

Western blot - Anti-TRIM2 antibody (AB3942)

Primary incubation was for 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-TRIM2 antibody (ab3942) at 0.5 µg/mL

All lanes:

Rat brain tissue lysate at 35 µg

Predicted band size: 82 kDa

Observed band size: 30 kDa,80 kDa,85 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human TRIM2 aa 700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9C040

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "6-12 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM2 also known as tripartite motif-containing protein 2 is a protein that plays an essential role in protein ubiquitination. It has a molecular mass of approximately 75 kDa. TRIM2 is mainly found in neurons and has expression profiles in the brain and other neural tissues. The protein contains a RING-type zinc finger domain that facilitates its E3 ubiquitin ligase activity enabling it to tag specific proteins for degradation by the proteasome system.

Biological function summary

TRIM2 functions by modulating the stability of specific proteins involved in neuronal growth and maintenance. It operates as part of ubiquitination complexes allowing for targeted protein degradation. Neurodevelopmental processes rely on TRIM2 activity to maintain cellular homeostasis. Its involvement in axonal transport and stabilization of microtubule-associated proteins underlines its important role in nervous system integrity and functionality.

Pathways

TRIM2's function integrates into the ubiquitin-proteasome pathway and neural development processes. Within the ubiquitin-proteasome pathway TRIM2 interacts closely with other E3 ligases and substrates essential for proper cell functioning. It also relates to the Neurotrophin TRK receptor signaling pathway where TRIM2 can influence neuronal survival and differentiation. These pathways connect TRIM2 to proteins like TRK receptors and other ligases that manage protein turnover and neuronal signaling.

Disrupted TRIM2 function associates with neurological conditions such as Charcot-Marie-Tooth disease and neurodegenerative disorders. Mutations in TRIM2 lead to impaired ubiquitination activities contributing to these diseases' progression. TRIM2 dysfunction also links to abnormalities in proteins like neurofilament light chain which further aggravate disease pathology by destabilizing neuronal architectures and interrupting normal neural processes.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

UBE2D1-dependent E3 ubiquitin-protein ligase that mediates the ubiquitination of NEFL and of phosphorylated BCL2L11. Plays a neuroprotective function. May play a role in neuronal rapid ischemic tolerance. Plays a role in antiviral immunity and limits New World arenavirus infection independently of its ubiquitin ligase activity (PubMed : 24068738).

See full target information TRIM2

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684673

2024

2'-Hydroxycinnamaldehyde, a Natural Product from Cinnamon, Alleviates Ischemia/Reperfusion-Induced Microvascular Dysfunction and Oxidative Damage in Rats by Upregulating Cytosolic BAG3 and Nrf2/HO-1.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Hsuan Cheng,Chih-Yao Chiang,Chung-Hsin Wu,Chiang-Ting Chien

International journal of molecular medicine 47: PubMed33760118

2021

Knockdown of long non‑coding RNA DLEU2 suppresses idiopathic pulmonary fibrosis by regulating the microRNA‑369‑3p/TRIM2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Hengzhong Yi,Danlin Luo,Yangbao Xiao,Di Jiang

International journal of oncology 53:1643-1656 PubMed30066883

2018

TRIM2 regulates the development and metastasis of tumorous cells of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Qin,Jichao Ye,Fulan Zhao,Shaoyu Hu,Suwei Wang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM2 antibody

Western blot - Anti-TRIM2 antibody (AB3942)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise