Anti-TRIM21/SS-A antibody [EPR20290] KO tested (ab207728)

Rabbit Recombinant Monoclonal TRIM21/SS-A antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

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Images

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (AB207728), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	IP	ICC/IF
Human	Tested	Tested	Not recommended	Not recommended
Mouse	Tested	Not recommended	Not recommended	Not recommended
Rat	Tested	Not recommended	Not recommended	Not recommended
Transfected cell line - Human	Not recommended	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/100 - 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Transfected cell line - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat, Transfected cell line - Human	Dilution info -	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell line - Human, Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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Target data

See full target information TRIM21

Function

E3 ubiquitin-protein ligase whose activity is dependent on E2 enzymes, UBE2D1, UBE2D2, UBE2E1 and UBE2E2 (PubMed:16297862, PubMed:16316627, PubMed:16472766, PubMed:16880511, PubMed:18022694, PubMed:18361920, PubMed:18641315, PubMed:18845142, PubMed:19675099, PubMed:26347139). Forms a ubiquitin ligase complex in cooperation with the E2 UBE2D2 that is used not only for the ubiquitination of USP4 and IKBKB but also for its self-ubiquitination (PubMed:16880511, PubMed:19675099). Component of cullin-RING-based SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complexes such as SCF(SKP2)-like complexes (PubMed:16880511). A TRIM21-containing SCF(SKP2)-like complex is shown to mediate ubiquitination of CDKN1B ('Thr-187' phosphorylated-form), thereby promoting its degradation by the proteasome (PubMed:16880511). Monoubiquitinates IKBKB that will negatively regulates Tax-induced NF-kappa-B signaling (PubMed:19675099). Negatively regulates IFN-beta production post-pathogen recognition by catalyzing polyubiquitin-mediated degradation of IRF3 (PubMed:18641315). Mediates the ubiquitin-mediated proteasomal degradation of IgG1 heavy chain, which is linked to the VCP-mediated ER-associated degradation (ERAD) pathway (PubMed:18022694). Promotes IRF8 ubiquitination, which enhanced the ability of IRF8 to stimulate cytokine genes transcription in macrophages (By similarity). Plays a role in the regulation of the cell cycle progression (PubMed:16880511). Enhances the decapping activity of DCP2 (PubMed:18361920). Exists as a ribonucleoprotein particle present in all mammalian cells studied and composed of a single polypeptide and one of four small RNA molecules (PubMed:1985094, PubMed:8666824). At least two isoforms are present in nucleated and red blood cells, and tissue specific differences in RO/SSA proteins have been identified (PubMed:8666824). The common feature of these proteins is their ability to bind HY RNAs.2 (PubMed:8666824). Involved in the regulation of innate immunity and the inflammatory response in response to IFNG/IFN-gamma (PubMed:26347139). Organizes autophagic machinery by serving as a platform for the assembly of ULK1, Beclin 1/BECN1 and ATG8 family members and recognizes specific autophagy targets, thus coordinating target recognition with assembly of the autophagic apparatus and initiation of autophagy (PubMed:26347139). Regulates also autophagy through FIP200/RB1CC1 ubiquitination and subsequent decreased protein stability (PubMed:36359729). Represses the innate antiviral response by facilitating the formation of the NMI-IFI35 complex through 'Lys-63'-linked ubiquitination of NMI (PubMed:26342464). During viral infection, promotes cell pyroptosis by mediating 'Lys-6'-linked ubiquitination of ISG12a/IFI27, facilitating its translocation into the mitochondria and subsequent CASP3 activation (PubMed:36426955). When up-regulated through the IFN/JAK/STAT signaling pathway, promotes 'Lys-27'-linked ubiquitination of MAVS, leading to the recruitment of TBK1 and up-regulation of innate immunity (PubMed:29743353). Mediates 'Lys-63'-linked polyubiquitination of G3BP1 in response to heat shock, leading to stress granule disassembly (PubMed:36692217).

Alternative names

RNF81, RO52, SSA1, TRIM21, E3 ubiquitin-protein ligase TRIM21, 52 kDa Ro protein, 52 kDa ribonucleoprotein autoantigen Ro/SS-A, RING finger protein 81, Ro(SS-A), Sjoegren syndrome type A antigen, Tripartite motif-containing protein 21, SS-A

Recommended products

Rabbit Recombinant Monoclonal TRIM21/SS-A antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 10 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR20290
Purification technique: Affinity purification Protein A
Specificity: This reagent is not recommended for mouse or rat IHC-P and human ICC/IF.
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

TRIM21 also known as SS-A or Ro52 is a 52 kDa protein that plays a role in the immune system. It functions as an E3 ubiquitin ligase involved in the ubiquitination process. This protein is expressed in various tissues including immune cells like lymphocytes and macrophages. TRIM21 recognizes autoantibodies linked to TRIM21 activity particularly those targeting the SS-A complex. Researchers often use antibodies like anti-TRIM21 or anti-SS-A to study its interactions and effects in cellular processes.

Biological function summary

TRIM21 contributes significantly to the regulation of immune responses and participates in innate and adaptive immunity. It forms part of a complex with other proteins to facilitate the degradation of viral particles through the ubiquitin-proteasome system a process known as antibody-dependent intracellular neutralization. As an important player in the immune defense TRIM21 oversees the timely removal of pathogens and prevents potential overactivation of immune responses that might harm the host.

Pathways

TRIM21 operates within the interferon signaling and NF-κB pathways two important areas of immune response modulation. TRIM21 interacts with molecules like transcription factors that influence the expression of interferon-responsive genes which are critical for pathogen defense. Its role in these pathways highlights its interactions with various immune-regulatory proteins helping to maintain immune system balance and effectiveness during infections.

Associated diseases and disorders

TRIM21 has strong associations with autoimmune conditions such as systemic lupus erythematosus and Sjögren's syndrome. Autoantibodies against TRIM21 are often used as biomarkers for these diseases and their presence can indicate increased autoimmunity. Relationship with other autoantigens like SS-A/Ro60 further connects TRIM21 with disease mechanisms suggesting its importance in the pathology of these autoimmune disorders. Understanding the role of TRIM21 in these diseases may improve diagnostic and therapeutic strategies.

Product promise

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8 product images

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Lanes 1-4: Merged signal (red and green). Green - ab207728 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab207728 Anti-TRIM21/SS-A antibody [EPR20290] was shown to specifically react with TRIM21/SS-A in wild-type A549 cells. Loss of signal was observed when knockout cell line Human TRIM21 (SS-A) knockout A549 cell line ab267024 (knockout cell lysate Human TRIM21 (SS-A) knockout A549 cell lysate ab257766) was used. Wild-type and TRIM21/SS-A knockout samples were subjected to SDS-PAGE. ab207728 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728) at 1/1000 dilution
Lane 1: Wild-type A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: TRIM21 knockout A549 (Human lung carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human TRIM21 (SS-A) knockout A549 cell line (Human TRIM21 (SS-A) knockout A549 cell line ab267024)
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 4: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Lanes 1-4: Merged signal (red and green). Green - ab207728 observed at 50 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.
ab207728 Anti-TRIM21/SS-A antibody [EPR20290] was shown to specifically react with TRIM21/SS-A in wild-type A549 cells. Loss of signal was observed when knockout cell line Human TRIM21 (SS-A) knockout A549 cell line ab267025 (knockout cell lysate Human TRIM21 (SS-A) knockout A549 cell lysate ab257767) was used. Wild-type and TRIM21/SS-A knockout samples were subjected to SDS-PAGE. ab207728 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728) at 1/500 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: TRIM21 knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human TRIM21 (SS-A) knockout A549 cell line (Human TRIM21 (SS-A) knockout A549 cell line ab267025)
Lane 3: HeLa cell lysate at 20 µg
Lane 4: MOLT-4 cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 54 kDa
Observed band size: 50 kDa
Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Lanes 1 - 4: Merged signal (red and green). Green - ab207728 observed at 50 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab207728 was shown to specifically react with in wild-type HAP1 cells as signal was lost in TRIM21 knockout cells. Wild-type and TRIM21 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. ab207728 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728) at 1/1000 dilution
Lane 1: Wild-type HAP1 whole cell lysate at 20 µg
Lane 2: TRIM21 knockout HAP1 whole cell lysate at 20 µg
Lane 3: HeLa whole cell lysate at 20 µg
Lane 4: MOLT-4 whole cell lysate at 20 µg
Predicted band size: 54 kDa
Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Blocking/Dilution buffer: 5% NFDM/TBST.
The level of TRIM21 expression can be elevated by IFN alpha treatment (PMID: 18071879).
All lanes: Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728) at 1/1000 dilution
Lane 1: Untreated HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 10 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) treated with 10 ng/ml human interferon-α (Recombinant human Interferon alpha 1 protein (Active) ab48750) for 16 hours at 10 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 50 kDa
Exposure time: 3min
Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728) at 1/1000 dilution
Lane 1: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen), whole cell lysate at 20 µg
Lane 2: MOLT-4 (human lymphoblastic leukemia cell line), whole cell lysate at 20 µg
Lane 3: Human fetal spleen lysate at 20 µg
Lane 4: Human fetal kidney lysate at 20 µg
Lane 5: Human thymus lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 50 kDa
Exposure time: 3min
Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Exposure times: Lane 1-2: 30 seconds; Lane 3: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728) at 1/1000 dilution
Lane 1: Rat spleen lysate at 20 µg
Lane 2: Rat thymus lysate at 20 µg
Lane 3: Mouse thymus lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 50 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Immunohistochemical analysis of paraffin-embedded A: Wild-type A549 (Human lung carcinoma epithelial cell) cell pellet, B: TRIM21 knockout A549 (Human HLA-E (HLA E) knockout A549 cell line ab267080) cell pellet labelling TRIM21/SS-A with ab207728 at 1/500 dilution (1.212 μg/ml) followed by LeicaDS9800 (Bond™ Polymer Refine Detection) secondary antibody at a ready to use concentration. Positive staining on (A) wild-type A549 cell pellet, no staining on (B) TRIM21 knockout A549 (Human HLA-E (HLA E) knockout A549 cell line ab267080) cell pellet. The section was incubated with ab207728 for 10 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)
Immunohistochemistry analysis of paraffin-embedded human tonsil tissue sections labelling TRIM21/SS-A with ab207728 at 1/100 dilution. The section was incubated with ab207728 for 10 mins at room temperature. Ready to use Leica DS9800 (Bond™ Polymer Refine Detection) was used as the secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 minutes.
Positive staining on human tonsil. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

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Anti-TRIM21/SS-A antibody [EPR20290]

Images

Publications

Key facts

Immunogen

Reactivity data

WB

TestedTested

Not recommendedNot recommended

IHC-P

TestedTested

Not recommendedNot recommended

IP

Not recommendedNot recommended

ICC/IF

Not recommendedNot recommended

Associated Products

5 products for Alternative Product

Target data

Function

Alternative names

Recommended products

Key facts

Storage

Notes

Supplementary info

Product promise

Tested

Expected

Predicted

Not recommended

8 product images

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Western blot - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM21/SS-A antibody [EPR20290] (ab207728)

Downloads

Product protocols

Tested
Tested

Not recommended
Not recommended

Tested
Tested

Not recommended
Not recommended

Not recommended
Not recommended

Not recommended
Not recommended