Rabbit Polyclonal TRIM22 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM22 aa 100-300.
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Publications
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- Journal of translational medicine 21:4432023Aberrant KAT2A accumulations render TRIM22-low melanoma sensitive to Notch1 inhibitors via epigenetic reprogramming.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoli Gu,Wei Min,Yibin Zeng,Ni Fan,Qihong QianPubMed 37415153
- Molecular therapy oncolytics 26:413-4282022TRIM22 orchestrates the proliferation of GBMs and the benefits of TMZ by coordinating the modification and degradation of RIG-I.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowei Fei,Xiuquan Wu,Ya-Nan Dou,Kai Sun,Qingdong Guo,Lei Zhang,Sanzhong Li,Jialiang Wei,Yu Huan,Xin He,Zhou FeiPubMed 36159777
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRIM22 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYM9
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
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Target data
Function
Interferon-induced E3 ubiquitin ligase that plays important roles in innate and adaptive immunity (PubMed:25683609, PubMed:35777501). Restricts the replication of many viruses including HIV-1, encephalomyocarditis virus (EMCV), hepatitis B virus (HBV), hepatitis C virus (HCV) or Zika virus (ZIKV) (PubMed:25683609, PubMed:35777501, PubMed:36042495). Mechanistically, negatively regulates HCV replication by promoting ubiquitination and subsequent degradation of viral NS5A (PubMed:25683609). Acts also by promoting the degradation of Zika virus NS1 and NS3 proteins through proteasomal degradation (PubMed:36042495). Acts as a suppressor of basal HIV-1 LTR-driven transcription by preventing Sp1 binding to the HIV-1 promoter (PubMed:26683615). Plays also a role in antiviral immunity by co-regulating together with NT5C2 the RIGI/NF-kappa-B pathway by promoting 'Lys-63'-linked ubiquitination of RIGI, while NT5C2 is responsible for 'Lys-48'-linked ubiquitination of RIGI (PubMed:36159777). Participates in adaptive immunity by suppressing the amount of MHC class II protein in a negative feedback manner in order to limit the extent of MHC class II induction (PubMed:35777501).
Alternative names
RNF94, STAF50, TRIM22, E3 ubiquitin-protein ligase TRIM22, 50 kDa-stimulated trans-acting factor, RING finger protein 94, RING-type E3 ubiquitin transferase TRIM22, Staf-50, Tripartite motif-containing protein 22
Recommended products
Rabbit Polyclonal TRIM22 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM22 aa 100-300.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRIM22 aa 100-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q8IYM9
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIM22 also known as Staf50 is a protein encoded by the TRIM22 gene. It weighs approximately 56 kDa. TRIM22 is expressed in different tissues throughout the body including the spleen thymus and liver. This protein belongs to the tripartite motif (TRIM) family which is characterized by a distinct structure comprising a RING finger B-box domains and a coiled-coil region. The presence of a RING finger domain suggests that TRIM22 might have E3 ubiquitin ligase activity.
- Biological function summary
TRIM22 plays a significant role in innate immunity influencing the antiviral response. As an E3 ubiquitin ligase it facilitates the ubiquitination and degradation of host proteins that are involved in viral replication processes. TRIM22 does not generally function in a protein complex but it interacts with other proteins in a cellular context. This interaction enhances the cellular response against viral infections by disrupting viral components and inhibiting viral replication.
- Pathways
TRIM22 is involved in the IFN (interferon) signaling pathway which is essential for the immune response against pathogens. In this pathway TRIM22 interacts with proteins like IFNAR1 and STAT1 acting as a mediator of antiviral activities. It also participates in the ubiquitin-proteasome system aiding in the regulation of protein turnover within the cell. These pathways demonstrate the broad scope of TRIM22 in maintaining cellular homeostasis and responding to external immune challenges.
- Associated diseases and disorders
TRIM22 has been linked to conditions such as systemic lupus erythematosus (SLE) and certain cancers. In SLE TRIM22 expression is often dysregulated contributing to an altered immune response. The protein also connects with STAT proteins which can be influential in autoimmune disease pathways. Moreover aberrant expression of TRIM22 has been observed in some cancer types suggesting a role in tumor biology and potentially offering a target for therapeutic intervention.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM22 antibody (ab224059)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human spleen tissue labeling TRIM22 with ab224059 at a 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-TRIM22 antibody (ab224059)
Immunocytochemical analysis of PFA-fixed, Triton X-100 permeabilized ASC TERT1 cells labeling TRIM22 with ab224059 at a 0.25 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM22 antibody (ab224059)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovary tissue labeling TRIM22 with ab224059 at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM22 antibody (ab224059)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human lymph node tissue labeling TRIM22 with ab224059 at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM22 antibody (ab224059)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling TRIM22 with ab224059 at a 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM22 antibody (ab224059)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human placenta tissue labeling TRIM22 with ab224059 at a 1/1000 dilution.
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