AB192032

Anti-TRIM23/ARD1 antibody [EPR7787]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal TRIM23/ARD1 antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 2 publications.

View Alternative Names

ARD1, ARFD1, RNF46, TRIM23, E3 ubiquitin-protein ligase TRIM23, ADP-ribosylation factor domain-containing protein 1, GTP-binding protein ARD-1, RING finger protein 46, RING-type E3 ubiquitin transferase TRIM23, Tripartite motif-containing protein 23

2 Images

Supplier Data

Western blot - Anti-TRIM23/ARD1 antibody [EPR7787] (AB192032)

All lanes:

Western blot - Anti-TRIM23/ARD1 antibody [EPR7787] (ab192032) at 1/50000 dilution

Lane 1:

HeLa lysate at 20 µg

Lane 2:

293T lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 64 kDa

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Supplier Data

Western blot - Anti-TRIM23/ARD1 antibody [EPR7787] (AB192032)

All lanes:

Western blot - Anti-TRIM23/ARD1 antibody [EPR7787] (ab192032) at 1/10000 dilution

Lane 1:

C6 lysate at 20 µg

Lane 2:

RAW 264.7 lysate at 20 µg

Lane 3:

PC12 lysate at 20 µg

Lane 4:

NIH 3T3 lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 64 kDa

false

Carrier free

Anti-TRIM23/ARD1 antibody [EPR7787] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7787

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM23 also known as ARD1 is a protein with an approximate mass of 77 kDa. It belongs to the tripartite motif (TRIM) family which is characterized by the presence of a RING finger domain a B-box domain and a coiled-coil region. TRIM23/ARD1 is expressed in various tissues including the heart and skeletal muscle. Its role as an E3 ubiquitin ligase is vital as it facilitates the ubiquitination and proteasomal degradation of specific substrates contributing to protein homeostasis within cells.

Biological function summary

TRIM23/ARD1 plays a role in immune and inflammatory responses. It participates in the maturation of autophagosomes a process that is essential for cellular repair and stress responses. Rather than functioning independently TRIM23/ARD1 associates with complex cellular systems including the endosomal-lysosomal pathway influencing the degradation and recycling of cellular components.

Pathways

TRIM23/ARD1's activity is integral to the autophagy and NF-kB signaling pathways. In the autophagy pathway TRIM23/ARD1 acts alongside proteins like ULK1 regulating autophagosomal formation and turnover. Within the NF-kB signaling pathway TRIM23/ARD1 modulates transcription factors that respond to inflammation and stress. Through these pathways TRIM23/ARD1 influences cellular responses to environmental stimuli ensuring proper cellular function.

TRIM23/ARD1's malfunctions have associations with cancer and neurodegenerative diseases. Its role in cancer involves the regulation of cell proliferation and apoptosis; disruptions can contribute to tumor growth. In neurodegenerative diseases TRIM23/ARD1 interacts with proteins such as tau impacting protein aggregation and clearance which are critical in diseases like Alzheimer's. Understanding TRIM23/ARD1's interactions in these conditions could provide insights into potential therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes (PubMed : 28871090).. (Microbial infection) Mediates TRAF6 auto-ubiquitination in the presence of human cytomegalovirus protein UL144, resulting in the virally controlled activation of NF-kappa-B stimulation at early times of HCMV infection.

See full target information TRIM23

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:113085 PubMed40777047

2025

Conditional deletion of TRPC1 channel modulates synaptic plasticity, long term depression, and memory extinction in Fragile X syndrome mice.

Applications

Unspecified application

Species

Unspecified reactive species

Farah Issa,Xavier Yerna,Thibaud Parpaite,Caren Jabbour,Olivier Schakman,Nicolas Tajeddine,Roberta Gualdani,Philippe Gailly

Journal of virology 93: PubMed31189704

2019

Disassembly of the TRIM23-TBK1 Complex by the Us11 Protein of Herpes Simplex Virus 1 Impairs Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Liu,Rachel Matrenec,Michaela U Gack,Bin He

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM23/ARD1 antibody [EPR7787]

Western blot - Anti-TRIM23/ARD1 antibody [EPR7787] (AB192032)

Western blot - Anti-TRIM23/ARD1 antibody [EPR7787] (AB192032)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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