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AB211300

Anti-TRIM24 antibody

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(1 Publication)

Rabbit Polyclonal TRIM24 antibody. Suitable for IHC-P, ICC, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRIM24.

View Alternative Names

RNF82, TIF1, TIF1A, TRIM24, Transcription intermediary factor 1-alpha, TIF1-alpha, E3 ubiquitin-protein ligase TRIM24, RING finger protein 82, RING-type E3 ubiquitin transferase TIF1-alpha, Tripartite motif-containing protein 24

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody (AB211300)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM24 antibody (AB211300)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labelling TRIM24 with ab211300 at 10μg/ml.

Immunocytochemistry - Anti-TRIM24 antibody (AB211300)
  • ICC

Supplier Data

Immunocytochemistry - Anti-TRIM24 antibody (AB211300)

Immunocytochemical analysis of EL4 (mouse) cells labelling TRIM24 with ab211300 at 2.5μg/ml.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM24 antibody (AB211300)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRIM24 antibody (AB211300)

Immunofluorescent analysis of EL4 (mouse) cells labeling TRIM24 with ab211300 at 20μg/ml.

Western blot - Anti-TRIM24 antibody (AB211300)
  • WB

Supplier Data

Western blot - Anti-TRIM24 antibody (AB211300)

Lane 1:

Western blot - Anti-TRIM24 antibody (ab211300) at 0.5 µg/mL

Lane 2:

Western blot - Anti-TRIM24 antibody (ab211300) at 1 µg/mL

All lanes:

EL4 cell lysate

Predicted band size: 116 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB, ICC, IHC-P

applications

Immunogen

Synthetic Peptide within Human TRIM24. The exact immunogen used to generate this antibody is proprietary information.

O15164

Specificity

At least two isoforms of TRIM24 are known to exist.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "2.5 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM24 also known as transcription intermediary factor 1-alpha (TIF1α) is a protein that acts mechanically as a transcriptional regulator. It has a molecular mass of approximately 140 kDa. This protein contains several domains including a tripartite motif (the eponymous TRIM) which consists of a RING domain B-box domains and a coiled-coil region. TRIM24 interacts with nuclear receptors and is expressed in various tissues but shows higher expression in the liver and lungs. It also plays a role in bridging the interaction between chromatin and transcription factors.
Biological function summary

TRIM24 is involved in transcriptional regulation by influencing gene expression. It acts as a co-regulator being part of large protein complexes. This protein interacts directly with histone tails to read histone marks and release transcriptional repression. TRIM24 also plays a role in ubiquitination and subsequent proteasomal degradation of specific proteins suggesting its involvement in maintaining protein homeostasis. Additionally TRIM24 has been shown to interact with p53 influencing cell cycle regulation and apoptosis.

Pathways

TRIM24 is involved in the regulation of the retinoic acid and vitamin D signaling pathways. It modulates the transcriptional activity of nuclear receptors through these pathways influencing cell proliferation and differentiation processes. TRIM24 does so by interacting with other proteins such as retinoid X receptor (RXR) and estrogen receptor (ER) establishing important crosstalk between signaling cascades critical for cell fate decisions.

TRIM24 has been implicated in the development of certain cancers such as breast cancer and liver cancer. In these contexts it functions as an oncogene promoting tumorigenesis through aberrant transcriptional regulation. TRIM24 can interact with other proteins associated with oncogenesis including p53 modulating its tumor suppressor functions. Furthermore deregulation of TRIM24 activity or expression levels has been correlated with inflammatory disorders highlighting its relevance in both cancer and inflammation-related conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional coactivator that interacts with numerous nuclear receptors and coactivators and modulates the transcription of target genes. Interacts with chromatin depending on histone H3 modifications, having the highest affinity for histone H3 that is both unmodified at 'Lys-4' (H3K4me0) and acetylated at 'Lys-23' (H3K23ac). Has E3 protein-ubiquitin ligase activity. During the DNA damage response, participates in an autoregulatory feedback loop with TP53. Early in response to DNA damage, ATM kinase phosphorylates TRIM24 leading to its ubiquitination and degradation. After sufficient DNA repair has occurred, TP53 activates TRIM24 transcription, ultimately leading to TRIM24-mediated TP53 ubiquitination and degradation (PubMed : 24820418). Plays a role in the regulation of cell proliferation and apoptosis, at least in part via its effects on p53/TP53 levels. Up-regulates ligand-dependent transcription activation by AR, GCR/NR3C1, thyroid hormone receptor (TR) and ESR1. Modulates transcription activation by retinoic acid (RA) receptors, including RARA. Plays a role in regulating retinoic acid-dependent proliferation of hepatocytes (By similarity). Also participates in innate immunity by mediating the specific 'Lys-63'-linked ubiquitination of TRAF3 leading to activation of downstream signal transduction of the type I IFN pathway (PubMed : 32324863). Additionally, negatively regulates NLRP3/CASP1/IL-1beta-mediated pyroptosis and cell migration probably by ubiquitinating NLRP3 (PubMed : 33724611).
See full target information TRIM24
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 11:3226-3237 PubMed31217891

2019

Epigenetic silencing of miR-137 induces resistance to bicalutamide by targeting TRIM24 in prostate cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yonghui Guan,Xiaoyue Guan,Hengqing An,Azhati Baihetiya,Wenguang Wang,Weimin Shao,Haiou Yang,Yujie Wang
View all publications
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