Skip to main content

Rabbit Recombinant Monoclonal TRIM25/EFP antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.

Be the first to review this product! Submit a review

Images

Western blot - Anti-TRIM25/EFP antibody [EPR7315] - BSA and Azide free (AB232356), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM25/EFP antibody [EPR7315] - BSA and Azide free (AB232356), expandable thumbnail
  • Immunoprecipitation - Anti-TRIM25/EFP antibody [EPR7315] - BSA and Azide free (AB232356), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-TRIM25/EFP antibody [EPR7315] - BSA and Azide free (AB232356), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TRIM25/EFP antibody [EPR7315] - BSA and Azide free (AB232356), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Expected
Rat
Expected
Tested
Expected
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Version

2 products for Alternative Product

Target data

Function

Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase (PubMed:16352599). Involved in innate immune defense against viruses by mediating ubiquitination of RIGI and IFIH1 (PubMed:17392790, PubMed:29357390, PubMed:30193849, PubMed:31710640, PubMed:33849980, PubMed:36045682). Mediates 'Lys-63'-linked polyubiquitination of the RIGI N-terminal CARD-like region and may play a role in signal transduction that leads to the production of interferons in response to viral infection (PubMed:17392790, PubMed:23950712). Mediates 'Lys-63'-linked polyubiquitination of IFIH1 (PubMed:30193849). Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway (PubMed:16352599, PubMed:17069755). Mediates estrogen action in various target organs (PubMed:22452784). Mediates the ubiquitination and subsequent proteasomal degradation of ZFHX3 (PubMed:22452784). Plays a role in promoting the restart of stalled replication forks via interaction with the KHDC3L-OOEP scaffold and subsequent ubiquitination of BLM, resulting in the recruitment and retainment of BLM at DNA replication forks (By similarity). Plays an essential role in the antiviral activity of ZAP/ZC3HAV1; an antiviral protein which inhibits the replication of certain viruses. Mechanistically, mediates 'Lys-63'-linked polyubiquitination of ZAP/ZC3HAV1 that is required for its optimal binding to target mRNA (PubMed:28060952, PubMed:28202764). Mediates also the ubiquitination of various substrates implicated in stress granule formation, nonsense-mediated mRNA decay, nucleoside synthesis and mRNA translation and stability (PubMed:36067236).

Alternative names

EFP, RNF147, ZNF147, TRIM25, E3 ubiquitin/ISG15 ligase TRIM25, Estrogen-responsive finger protein, RING finger protein 147, RING-type E3 ubiquitin transferase, RING-type E3 ubiquitin transferase TRIM25, Tripartite motif-containing protein 25, Ubiquitin/ISG15-conjugating enzyme TRIM25, Zinc finger protein 147

Recommended products

Rabbit Recombinant Monoclonal TRIM25/EFP antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR7315
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab232356 is the carrier-free version of Anti-TRIM25/EFP antibody [EPR7315] ab167154.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRIM25 also known as EFP is a protein involved in antiviral response mechanisms. TRIM25 has a molecular mass of approximately 70 kDa and is part of the TRIM (Tripartite Motif) family which contains three distinct zinc-binding domains followed by a coiled-coil region. This protein exhibits strong expression in a variety of tissues including lung liver and intestines. As a member of the E3 ubiquitin ligase enzyme family TRIM25 plays a direct role in protein ubiquitination which tags specific proteins for degradation by the proteasome.

Biological function summary

TRIM25 functions as a significant modulator of the innate immune response against viral infections. In cells it acts within the RIG-I signaling complex directly promoting the RIG-I-MAVS pathway that detects viral RNA. TRIM25 mediates the ubiquitination of RIG-I allowing for its proper activation and subsequent signal transduction facilitating a robust antiviral state. TRIM25's role extends beyond antiviral defenses to include regulatory functions in cell proliferation and differentiation.

Pathways

TRIM25 engages actively in the innate immune signaling and JAK-STAT pathways. It interacts and cooperates with RIG-I in the recognition and response to viral tRNA leading to the production of interferons that establish an antiviral environment. Furthermore TRIM25 also connects with proteins like TRIM21 which further engages the immune response. This interaction enhances the overall ability of cells to fend off viral pathogens efficiently.

Associated diseases and disorders

Altered TRIM25 function is associated with conditions like systemic lupus erythematosus (SLE) and various cancers. In the context of SLE TRIM25's regulatory role in innate immunity can affect the severity and progression of this autoimmune disorder. Additionally in some cancers abnormal TRIM25 expression can influence cell cycle regulation and metastasis. Through its ubiquitination activities TRIM25 interacts directly with proteins such as p53 affecting tumor suppression pathways and contributing to the complexity of disease development and progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com