Mouse Monoclonal TRIM27 antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM27.
IgG2a
Mouse
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA
Liquid
Monoclonal
ICC | Flow Cyt | Protein Array | |
---|---|---|---|
Human | Tested | Tested | Expected |
Mouse | Predicted | Predicted | Predicted |
Rat | Predicted | Predicted | Predicted |
Recombinant full length protein - Human | Not recommended | Not recommended | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1-2 µg for 106 Cells | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Recombinant full length protein - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
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E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.
Zinc finger protein RFP, RING finger protein 76, RING-type E3 ubiquitin transferase TRIM27, Ret finger protein, Tripartite motif-containing protein 27, RNF76, RFP, TRIM27
Mouse Monoclonal TRIM27 antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM27.
Zinc finger protein RFP, RING finger protein 76, RING-type E3 ubiquitin transferase TRIM27, Ret finger protein, Tripartite motif-containing protein 27, RNF76, RFP, TRIM27
IgG2a
Mouse
Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA
Liquid
Monoclonal
PCRP-TRIM27-1B3
Affinity purification Protein A/G
Purified from bioreactor concentrate
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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Immunofluorescence analysis of PFA-fixed U87 (human glioblastoma-astrocytoma epithelial cell line) cells labeling TRIM27-1B3 with ab277103 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).
Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TRIM27 using ab277103 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).
Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277103.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
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