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Mouse Monoclonal TRIM27 antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM27.

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Images

Immunocytochemistry - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (AB277103), expandable thumbnail
  • Flow Cytometry - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (AB277103), expandable thumbnail
  • Protein Array - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (AB277103), expandable thumbnail

Publications

Key facts

Isotype

IgG2a

Host species

Mouse

Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 99.9% PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human TRIM27. Database link P14373

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICCFlow CytProtein Array
Human
Tested
Tested
Expected
Mouse
Predicted
Predicted
Predicted
Rat
Predicted
Predicted
Predicted
Recombinant full length protein - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

1-2 µg/mL

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1-2 µg for 106 Cells

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Recombinant full length protein - Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Version

Target data

Function

E3 ubiquitin-protein ligase that mediates ubiquitination of PIK3C2B and inhibits its activity; mediates the formation of 'Lys-48'-linked polyubiquitin chains; the function inhibits CD4 T-cell activation. Acts as a regulator of retrograde transport: together with MAGEL2, mediates the formation of 'Lys-63'-linked polyubiquitin chains at 'Lys-220' of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853). Has a transcriptional repressor activity by cooperating with EPC1. Induces apoptosis by activating Jun N-terminal kinase and p38 kinase and also increases caspase-3-like activity independently of mitochondrial events. May function in male germ cell development. Has DNA-binding activity and preferentially bound to double-stranded DNA.

Alternative names

Recommended products

Mouse Monoclonal TRIM27 antibody. Suitable for ICC, Flow Cyt, Protein Array and reacts with Human, Recombinant full length protein - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM27.

Alternative names

Key facts

Isotype

IgG2a

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human TRIM27. Database link P14373
Clone number

PCRP-TRIM27-1B3

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from bioreactor concentrate

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (ab277103), expandable thumbnail

    Immunocytochemistry - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (ab277103)

    Immunofluorescence analysis of PFA-fixed U87 (human glioblastoma-astrocytoma epithelial cell line) cells labeling TRIM27-1B3 with ab277103 at 2 μg/ml, followed by goat anti-mouse IgG-CF488 (green); phalloidin counterstain (red).

  • Flow Cytometry - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (ab277103), expandable thumbnail

    Flow Cytometry - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (ab277103)

    Flow cytometry analysis of PFA-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling TRIM27 using ab277103 at 2 μg / 106 cells followed by goat anti-mouse IgG-CF488 (blue); unstained cells (red).

  • Protein Array - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (ab277103), expandable thumbnail

    Protein Array - Anti-TRIM27 antibody [PCRP-TRIM27-1B3] (ab277103)

    Analysis of Protein Array containing more than 19,000 full-length human proteins using ab277103.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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