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AB244380

Anti-TRIM29 antibody

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(4 Publications)

Rabbit Polyclonal TRIM29 antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Rat samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM29 aa 50-200.

View Alternative Names

ATDC, TRIM29, Tripartite motif-containing protein 29, Ataxia telangiectasia group D-associated protein

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human skin tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human stomach tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

No positivity in glandular cells as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human prostate tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM29 antibody (AB244380)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIM29 antibody (AB244380)

PFA-fixed, Triton X-100 permeabilized SiHa cells stained for TRIM29 (green) using ab244380 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human esophagus tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM29 antibody (AB244380)

Paraffin-embedded human placenta tissue stained for TRIM29 using ab244380 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-TRIM29 antibody (AB244380)
  • WB

Unknown

Western blot - Anti-TRIM29 antibody (AB244380)

All lanes:

Western blot - Anti-TRIM29 antibody (ab244380) at 0.4 µg/mL

All lanes:

RT4 (Human urinary bladder cancer cell line) cell lysate

Predicted band size: 65 kDa

false

Western blot - Anti-TRIM29 antibody (AB244380)
  • WB

Unknown

Western blot - Anti-TRIM29 antibody (AB244380)

All lanes:

Western blot - Anti-TRIM29 antibody (ab244380) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat cell line) cell lysate

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human TRIM29 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

Q14134

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The tripartite motif-containing protein 29 (TRIM29) also known as ATDC is a protein with a molecular mass of approximately 66 kDa. TRIM29 is part of the TRIM family characterized by its RING finger motif B-box type 1 and 2 domains and a coiled-coil region which are significant for its involvement in ubiquitination processes. Researchers have found TRIM29 expression largely in epithelial tissues exhibiting varied levels of expression depending on tissue type.
Biological function summary

TRIM29 functions as an essential regulator of DNA damage responses and cellular proliferation. It is not found as part of any larger protein complex but interacts with various other proteins to carry out its function. TRIM29 can influence the activity of key molecules involved in DNA repair particularly through its interaction with DNA-dependent protein kinase catalytic subunit (DNA-PKcs) which enhances cell survival after genotoxic stress. It participates in ensuring proper functioning of cell cycle checkpoints and modulation of apoptosis.

Pathways

TRIM29 participates in the DNA damage response pathway and the WNT signaling pathway. It interacts with proteins such as β-catenin which influences the transcriptional regulation of target genes involved in cell proliferation. Through these interactions TRIM29 influences cellular responses to DNA damage and the regulation of cell growth linking it to critical pathways that maintain genomic stability and cell integrity.

Aberrant TRIM29 expression correlates with various cancers including breast and colorectal cancers. In breast cancer TRIM29 affects the pathways involving p53 a known tumor suppressor protein. In colorectal cancer its overexpression is associated with enhanced cancer cell proliferation and survival often in conjunction with KRAS mutations. TRIM29's regulatory role in these pathways highlights its potential as a biomarker and a therapeutic target in oncology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a crucial role in the regulation of macrophage activation in response to viral or bacterial infections within the respiratory tract. Mechanistically, TRIM29 interacts with IKBKG/NEMO in the lysosome where it induces its 'Lys-48' ubiquitination and subsequent degradation. In turn, the expression of type I interferons and the production of pro-inflammatory cytokines are inhibited. Additionally, induces the 'Lys-48' ubiquitination of STING1 in a similar way, leading to its degradation.
See full target information TRIM29
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PeerJ 12:e16921 PubMed38426148

2024

Identification and immunoinfiltration analysis of key genes in ulcerative colitis using WGCNA.

Applications

Unspecified application

Species

Unspecified reactive species

Siyi Ni,Yingchao Liu,Jihong Zhong,Yan Shen

Cell biology international 47:1126-1135 PubMed36841942

2023

TRIM29 promotes podocyte pyroptosis in diabetic nephropathy through the NF-kB/NLRP3 inflammasome pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohong Xu,Zihan Qin,Ce Zhang,Xia Mi,Chi Zhang,Feihong Zhou,Junsheng Wang,Liexiang Zhang,Fei Hua

Molecular medicine reports 22:3822-3832 PubMed32901838

2020

Long non‑coding RNA TP73‑AS1 accelerates the progression and cisplatin resistance of non‑small cell lung cancer by upregulating the expression of TRIM29 via competitively targeting microRNA‑34a‑5p.

Applications

Unspecified application

Species

Unspecified reactive species

Shunxiang Luo,Ming Shen,Hui Chen,Weiwei Li,Cong Chen

Cancer management and research 12:4981-4990 PubMed32612385

2020

miRNA-181a-5p Enhances the Sensitivity of Cells to Cisplatin in Esophageal Adenocarcinoma by Targeting CBLB.

Applications

Unspecified application

Species

Unspecified reactive species

Song Yang,Peng Wang,Songhua Wang,Aihua Cong,Qi Zhang,Wenhao Shen,Xiangyi Li,Wei Zhang,Gaohua Han
View all publications
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