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AB111840

Anti-TRIM3 antibody

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(7 Publications)

Rabbit Polyclonal TRIM3 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM3.

View Alternative Names

BERP, RNF22, RNF97, TRIM3, Tripartite motif-containing protein 3, Brain-expressed RING finger protein, RING finger protein 22, RING finger protein 97

1 Images
Western blot - Anti-TRIM3 antibody (AB111840)
  • WB

Unknown

Western blot - Anti-TRIM3 antibody (AB111840)

All lanes:

Western blot - Anti-TRIM3 antibody (ab111840) at 1/1000 dilution

Lane 1:

Human fetal brain lysate

Lane 2:

Human fetal heart lysate

Lane 3:

293T lysate

Lane 4:

RAW264.7 lysate

Lane 5:

LoVo lysate

Predicted band size: 81 kDa

Observed band size: 81 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TRIM3. The exact immunogen used to generate this antibody is proprietary information.

O75382

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute in 200 µL of water
Purification technique
Affinity purification Immunogen
Purification notes
ab111840 is purified by a peptide affinity column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM3 also known as Tripartite Motif Containing 3 is a member of the tripartite motif family of proteins and has a mass of approximately 70 kDa. This protein functions mechanically as an E3 ubiquitin-protein ligase involved in the tagging of substrates for proteasomal degradation. TRIM3 is expressed in various tissues though it shows higher levels in the brain particularly in neurons. The presence of TRIM3 in neuronal tissues suggests an important role in neural function and homeostasis.
Biological function summary

TRIM3 influences key cellular processes such as cell cycle progression and apoptosis. It is part of a multimeric protein complex that regulates the degradation of certain protein substrates. By modulating the ubiquitin-proteasome system TRIM3 contributes to maintaining protein homeostasis within cells. The ubiquitin ligase activity of TRIM3 ensures that misfolded or damaged proteins are correctly identified and directed towards degradation which is essential for normal cell function.

Pathways

TRIM3 integrates into critical signaling networks that maintain cellular balance and response. It plays a significant role in the ubiquitin-proteasome pathway which is a major cellular mechanism for controlling protein levels. TRIM3 interacts with several proteins in this pathway including the ubiquitin-conjugating enzymes to exert its regulatory effects. Additionally TRIM3 has connections with other pathways involved in neural development and function intersecting with proteins such as neurofilament chains.

TRIM3 has been linked to neurodegenerative conditions and cancer. In Alzheimer's disease for example TRIM3 associates with tau protein possibly affecting its degradation and accumulation which contributes to disease pathology. There is also evidence of TRIM3 involvement in glioblastoma where its regulatory role in protein turnover may influence cell proliferation and tumor progression. The modulation of TRIM3 activity or its interactions in these conditions could provide valuable insights into therapeutic targets.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin ligase that plays essential roles in neuronal functions such as regulation of neuronal plasticity, learning, and memory (By similarity). In addition to its neuronal functions, participates in other biological processes such as innate immunity or cell cycle regulation. Component of the cytoskeleton-associated recycling or transport complex in neurons, polyubiquitinates gamma-actin, thus regulating neuronal plasticity, learning, and memory (By similarity). Ubiquitinates postsynaptic scaffold GKAP, a neuronal substrate involved in synaptic remodeling and thereby modulates dendritic spine morphology (By similarity). Positively regulates motility of microtubule-dependent motor protein KIF21B (By similarity). Induces growth arrest via its RING-dependent E3 ligase activity and ubiquinates CDKN1A (PubMed : 24393003). Positively regulates TLR3-mediated signaling by mediating 'Lys-63'-linked polyubiquitination of TLR3 (PubMed : 32878999). In turn, promotes the recognition and sorting of polyubiquitinated TLR3 by the ESCRT complexes (PubMed : 32878999).
See full target information TRIM3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:27264 PubMed40715334

2025

TRIM3 suppressed the tumorigenicity of melanoma cells by ubiquitinating YAP1.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Tong Wang,Jing-Chen Zhao,Ding-Ming Song,Jun-Jiang Chen

Cell death and differentiation 31:53-64 PubMed37978273

2023

TRIM3 facilitates ferroptosis in non-small cell lung cancer through promoting SLC7A11/xCT K11-linked ubiquitination and degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhangjie Wang,Na Shen,Ziao Wang,Lei Yu,Song Yang,Yang Wang,Yu Liu,Gaohua Han,Qi Zhang

Cell reports 42:112633 PubMed37314927

2023

Phosphatidylinositol 4-kinase IIα is a glycogen synthase kinase 3-regulated interaction hub for activity-dependent bulk endocytosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eva-Maria Blumrich,Jessica C Nicholson-Fish,Marie Pronot,Elizabeth C Davenport,Dominic Kurian,Adam Cole,Karen J Smillie,Michael A Cousin

Nature communications 13:7583 PubMed36481767

2022

Divergent self-association properties of paralogous proteins TRIM2 and TRIM3 regulate their E3 ligase activity.

Applications

Unspecified application

Species

Unspecified reactive species

Diego Esposito,Jane Dudley-Fraser,Acely Garza-Garcia,Katrin Rittinger

OncoTargets and therapy 12:11587-11595 PubMed32021240

2019

TRIM3 Negatively Regulates Autophagy Through Promoting Degradation of Beclin1 in Ewing Sarcoma Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qunshan Lu,Yuankai Zhang,Liang Ma,Deqiang Li,Ming Li,Peilai Liu,Jianmin Li

Biochemical and biophysical research communication 516:872-879 PubMed31270028

2019

miR-454-3p promotes proliferation and induces apoptosis in human cervical cancer cells by targeting TRIM3.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Song,Qisang Guo,Shujun Gao,Keqin Hua

American journal of translational research 11:2431-2438 PubMed31105849

2019

miR-4513 promotes breast cancer progression through targeting TRIM3.

Applications

Unspecified application

Species

Unspecified reactive species

Yongzhen Li,Huifang Zhu,Jianqiang Wang,Xinlai Qian,Na Li
View all publications
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