JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB76953

Anti-TRIM30/Rpt1 antibody

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal TRIM30/Rpt1 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Mouse Trim30a.

View Alternative Names

Rpt-1, Rpt1, Trim30, Trim30a, Tripartite motif-containing protein 30A, Down regulatory protein of interleukin-2 receptor, Tripartite motif-containing protein 30

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM30/Rpt1 antibody (AB76953)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM30/Rpt1 antibody (AB76953)

ab76953 at 10 μg/ml staining TRIM30/Rpt1 in mouse ovary tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

Western blot - Anti-TRIM30/Rpt1 antibody (AB76953)
  • WB

Supplier Data

Western blot - Anti-TRIM30/Rpt1 antibody (AB76953)

Lane 1:

Western blot - Anti-TRIM30/Rpt1 antibody (ab76953) at 1 µg/mL

Lane 2:

Western blot - Anti-TRIM30/Rpt1 antibody (ab76953) at 2 µg/mL

All lanes:

Mouse heart tissue lysate

Predicted band size: 57 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Trim30a. The exact immunogen used to generate this antibody is proprietary information.

P15533

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM30 also known as Rpt1 is a member of the tripartite motif (TRIM) family of proteins characterized by the presence of a RING finger B-box and coiled-coil domains. In humans TRIM30 has a molecular mass of about 56 kDa. The protein primarily resides in the cytoplasm and nucleus suggesting its involvement in various cellular processes. Expression of TRIM30 is observed across different tissues with varying levels depending on cell type and tissue-specific functions.
Biological function summary

The protein TRIM30 acts as an E3 ubiquitin ligase an important component in the regulation of protein degradation processes. It facilitates the attachment of ubiquitin molecules to specific substrate proteins targeting them for proteasomal degradation and modulating various cellular activities. TRIM30 associates with several complexes involved in immune responses and acts as a regulatory factor in innate immunity. Through ubiquitination TRIM30 influences signaling pathways and cellular responses to external stimuli maintaining cellular homeostasis.

Pathways

TRIM30 influences signal transduction by interacting with important molecules in pathways like the NF-kB signaling and MAPK pathways. In the NF-kB signaling pathway TRIM30 can modulate the activation of NF-kB impacting the expression of pro-inflammatory cytokines and immune responses. Within the MAPK pathway this modulation affects cellular responses like proliferation and apoptosis. TRIM30 thereby interacts with other proteins such as TAB2 and TAK1 shaping the downstream consequences of these pathways.

TRIM30 has connections with conditions related to immune dysregulation and inflammation such as systemic lupus erythematosus and rheumatoid arthritis. Its role in controlling inflammatory signaling makes it significant in the progression of these disorders. Within lupus for example abnormal regulation of TRIM30 might contribute to hyperactive immune responses. TRIM30's involvement in these diseases links it to proteins such as NF-kB and other components in inflammatory pathways dictating the severity and progression of the conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Trans-acting factor that regulates gene expression of interleukin 2 receptor alpha chain. May affect IL2R-alpha expression through cis-acting negative regulatory elements or through competition with proteins that bind to enhancer or activator sequences. Negatively regulates Toll-like receptor (TLR)-mediated activation of NFKB by promoting degradation of TAB2 and TAB3 and preventing TRAF6 autoubiquitination. Negatively regulates production of reactive oxygen species (ROS) which inhibits activation of the NLRP3 inflammasome complex. This, in turn, regulates activation of CASP1 and subsequent cleavage of IL1B and IL18. No activity detected against a range of retroviruses including a number of lentiviruses, gammaretroviruses and betaretroviruses.
See full target information Trim30a
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:28 PubMed33558529

2021

HMGB1 orchestrates STING-mediated senescence via TRIM30α modulation in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Je-Jung Lee,In Ho Park,Man Sup Kwak,Woo Joong Rhee,Songhee H Kim,Jeon-Soo Shin

PloS one 9:e95805 PubMed24756037

2014

Tripartite motif-containing protein 30 modulates TCR-activated proliferation and effector functions in CD4+ T cells.

Applications

Flow Cyt

Species

Mouse

Un Yung Choi,Ji Yeon Hur,Myeong Sup Lee,Quanri Zhang,Won Young Choi,Lark Kyun Kim,Wook-Bin Lee,Goo Taeg Oh,Young-Joon Kim
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB254715

Anti-ZNF503 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF503 antibody (AB254715)

  • 0
  • 0
Primary Antibodies

AB273052

Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ZWINT antibody [EPR23440-96] - BSA and Azide free (AB273052)

  • 0
  • 0
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com