Rabbit Polyclonal TRIM32 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM32 aa 1-300.
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- Cell & bioscience 12:1832022mTORC1-c-Myc pathway rewires methionine metabolism for HCC progression through suppressing SIRT4 mediated ADP ribosylation of MAT2A.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLiang Zhao,Huizhao Su,Xiaomeng Liu,Hongquan Wang,Yukuan Feng,Yan Wang,Haiqiang Chen,Luo Dai,Shihui Lai,Siqi Xu,Chong Li,Jihui Hao,Bo TangPubMed 36371321
- Cell reports 30:98-111.e52020TRIM32/USP11 Balances ARID1A Stability and the Oncogenic/Tumor-Suppressive Status of Squamous Cell Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQingyu Luo,Xiaowei Wu,Yabing Nan,Wan Chang,Pengfei Zhao,Yiping Zhang,Dan Su,Zhihua LiuPubMed 31914402
- Genome biology 20:2622019eIF4A2 drives repression of translation at initiation by Ccr4-Not through purine-rich motifs in the 5'UTR.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnia Wilczynska et. al.PubMed 31791371
- Cells 8:2019Analysis of the Zn-Binding Domains of TRIM32, the E3 Ubiquitin Ligase Mutated in Limb Girdle Muscular Dystrophy 2H.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElisa Lazzari et. al.PubMed 30884854
- Oncology letters 13:3131-31382017Overexpression of E3 ubiquitin ligase tripartite motif 32 correlates with a poor prognosis in patients with gastric cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasahiro Ito,Kazuhiro Migita,Sohei Matsumoto,Kohei Wakatsuki,Tetsuya Tanaka,Tomohiro Kunishige,Hiroshi Nakade,Mitsuhiro Nakatani,Yoshiyuki NakajimaPubMed 28521418
- The Journal of biological chemistry 290:13736-482015MicroRNA-155 Reinforces HIV Latency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDebbie S Ruelas et. al.PubMed 25873391
- Oncogenesis 3:e902014Synergistic effects of proteasome inhibitor carfilzomib in combination with tyrosine kinase inhibitors in imatinib-sensitive and -resistant chronic myeloid leukemia models.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRIM32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13049
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
| Cow | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/250 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow | Dilution info - | Notes - |
Target data
Function
E3 ubiquitin ligase that plays a role in various biological processes including neural stem cell differentiation, innate immunity, inflammatory resonse and autophagy (PubMed:19349376, PubMed:31123703). Plays a role in virus-triggered induction of IFN-beta and TNF-alpha by mediating the ubiquitination of STING1. Mechanistically, targets STING1 for 'Lys-63'-linked ubiquitination which promotes the interaction of STING1 with TBK1 (PubMed:22745133). Regulates bacterial clearance and promotes autophagy in Mycobacterium tuberculosis-infected macrophages (PubMed:37543647). Negatively regulates TLR3/4-mediated innate immune and inflammatory response by triggering the autophagic degradation of TICAM1 in an E3 activity-independent manner (PubMed:28898289). Plays an essential role in oxidative stress induced cell death by inducing loss of transmembrane potential and enhancing mitochondrial reactive oxygen species (ROS) production during oxidative stress conditions (PubMed:32918979). Ubiquitinates XIAP and targets it for proteasomal degradation (PubMed:21628460). Ubiquitinates DTNBP1 (dysbindin) and promotes its degradation (PubMed:19349376). May ubiquitinate BBS2 (PubMed:22500027). Ubiquitinates PIAS4/PIASY and promotes its degradation in keratinocytes treated with UVB and TNF-alpha (By similarity). Also acts as a regulator of autophagy by mediating formation of unanchored 'Lys-63'-linked polyubiquitin chains that activate ULK1: interaction with AMBRA1 is required for ULK1 activation (PubMed:31123703). Positively regulates dendritic branching by promoting ubiquitination and subsequent degradation of the epigenetic factor CDYL (PubMed:34888944). Under metabolic stress and phosphorylation by CHK2, mediates 'Lys-63'-linked ubiquitination of ATG7 at 'Lys-45' to initiate autophagy (PubMed:37943659). (Microbial infection) May play a significant role in mediating the biological activity of the HIV-1 Tat protein in vivo (PubMed:7778269). Binds specifically to the activation domain of HIV-1 Tat and can also interact with the HIV-2 and EIAV Tat proteins in vivo (PubMed:7778269).
Alternative names
HT2A, TRIM32, E3 ubiquitin-protein ligase TRIM32, 72 kDa Tat-interacting protein, RING-type E3 ubiquitin transferase TRIM32, Tripartite motif-containing protein 32, Zinc finger protein HT2A
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Rabbit Polyclonal TRIM32 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM32 aa 1-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRIM32 aa 1-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q13049
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIM32 also known as Tripartite Motif Containing 32 is a protein that functions as an E3 ubiquitin ligase. It plays a role in attaching ubiquitin molecules to proteins tagging them for degradation by the proteasome. TRIM32 consists of a RING domain a B-box and a coiled-coil region which are characteristic of the TRIM family. The molecular mass of TRIM32 is approximately 71 kDa. It is expressed in various tissues with notable expression in skeletal muscle and the heart implying an important role in muscle physiology.
- Biological function summary
TRIM32 participates in regulating muscle cell differentiation and proliferation. It interacts with several proteins including dysbindin and myospryn suggesting its involvement in a multi-protein complex. TRIM32 has a regulatory role in muscle maintenance where it influences the stability and function of muscle proteins. Moreover it is also involved in neurogenesis affecting neuronal differentiation and survival emphasizing its role beyond muscle tissues.
- Pathways
TRIM32 plays a significant role in the PI3K/Akt signaling pathway and the TGF-beta signaling pathway. In the PI3K/Akt pathway TRIM32 modulates cell growth and survival interacting with proteins such as Akt and mTOR. In the context of TGF-beta signaling TRIM32 can influence cellular processes like proliferation and differentiation possibly by interacting with SMAD proteins. These interactions indicate its importance in cellular growth and differentiation pathways.
- Associated diseases and disorders
TRIM32 is linked with limb-girdle muscular dystrophy type 2H and certain forms of sarcopenia. Mutations in TRIM32 cause dysfunction in muscle protein turnover leading to muscle deterioration and weakness. In limb-girdle muscular dystrophy TRIM32 mutations disrupt its interaction with dysbindin impairing muscle structure. Furthermore TRIM32 might be involved in neurodegenerative diseases with connections to proteins like alpha-synuclein hinting at a potential role in Parkinson's disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-TRIM32 antibody (ab96612)
7.5% SDS PAGE.
All lanes: Western blot - Anti-TRIM32 antibody (ab96612) at 1/1000 dilution
All lanes: 293T whole cell lysate at 30 µg
Predicted band size: 72 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM32 antibody (ab96612)
ab96612 at 1/100 dilution staining TRIM32 in H441 Xenograft by Immunohistochemistry, Paraffin-embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-TRIM32 antibody (ab96612)
Immunofluorescence analysis of paraformaldehyde-fixed A431, using TRIM32(ab96612) antibody at 1:500 dilution.
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