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AB264174

Anti-TRIM33 antibody

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Rabbit Polyclonal TRIM33 antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human TRIM33 aa 1-50.

View Alternative Names

KIAA1113, RFG7, TIF1G, TRIM33, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, RING-type E3 ubiquitin transferase TRIM33, Transcription intermediary factor 1-gamma, Tripartite motif-containing protein 33, Protein Rfg7, TIF1-gamma

1 Images
Western blot - Anti-TRIM33 antibody (AB264174)
  • WB

Supplier Data

Western blot - Anti-TRIM33 antibody (AB264174)

4-8% SDS-PAGE.

All lanes:

Western blot - Anti-TRIM33 antibody (ab264174) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 122 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TRIM33 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9UPN9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM33 also known as TIF1γ is an E3 ubiquitin ligase involved in protein ubiquitination influencing protein stability and activity. It has a molecular mass of approximately 135 kDa. TRIM33's expression occurs widely in human tissues with higher levels found in the spleen thymus and testis. The protein contains a RING finger domain B-box type zinc fingers and coiled-coil region which are typical for the TRIM family.
Biological function summary

The ubiquitination actions of TRIM33 modulate transcriptional regulation and cell fate decisions. TRIM33 interacts with transcription factors and chromatin often forming part of the larger transcriptional regulatory complexes. Its role in repressing TGF-β signaling by ubiquitylating SMAD proteins highlights its influence on cellular growth and differentiation.

Pathways

TRIM33 has an impact on the TGF-β and BMP signaling pathways both essential for controlling cell proliferation and apoptosis. It acts in partnership with other proteins such as SMAD4 to negatively regulate TGF-β responses helping balance cellular growth. TRIM33 also interfaces with other pathway regulators like p53 influencing cell cycle and repair mechanisms.

TRIM33 is linked to cancer and fibrosis. Mutations or deregulation of TRIM33 contribute to the development of leukemia where it interacts with proteins like AML1-ETO promoting leukemogenesis through altered transcriptional regulation. In pulmonary fibrosis TRIM33's interaction with SMAD3 illustrates its role in pathological signaling highlighting therapeutic potential in modulating its activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed : 16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
See full target information TRIM33
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Product promise

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