AB84455

Anti-TRIM33 antibody

Name: Anti-TRIM33 antibody (ab84455) | Abcam
Brand: Abcam Limited
SKU: AB84455
Price: 490 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(4 Publications)

Rabbit Polyclonal TRIM33 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM33 aa 1050 to C-terminus.

View Alternative Names

KIAA1113, RFG7, TIF1G, TRIM33, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, RING-type E3 ubiquitin transferase TRIM33, Transcription intermediary factor 1-gamma, Tripartite motif-containing protein 33, Protein Rfg7, TIF1-gamma

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Mouse teratoma tissue using 1/250 ab84455, followed by DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human colon adenocarcinoma tissue using 1/250 ab84455, followed by DAB staining.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

Immunohistochemistry analysis of formalin-fixed, paraffin-embedded Human prostate adenocarcinoma using 1/250 ab84455, followed by DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human TRIM33 aa 1050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UPN9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Antigen retrieval is not essential but may optimise staining." }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chimpanzee": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Guinea pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Platypus": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Turkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM33 also known as TIF1γ is an E3 ubiquitin ligase involved in protein ubiquitination influencing protein stability and activity. It has a molecular mass of approximately 135 kDa. TRIM33's expression occurs widely in human tissues with higher levels found in the spleen thymus and testis. The protein contains a RING finger domain B-box type zinc fingers and coiled-coil region which are typical for the TRIM family.

Biological function summary

The ubiquitination actions of TRIM33 modulate transcriptional regulation and cell fate decisions. TRIM33 interacts with transcription factors and chromatin often forming part of the larger transcriptional regulatory complexes. Its role in repressing TGF-β signaling by ubiquitylating SMAD proteins highlights its influence on cellular growth and differentiation.

Pathways

TRIM33 has an impact on the TGF-β and BMP signaling pathways both essential for controlling cell proliferation and apoptosis. It acts in partnership with other proteins such as SMAD4 to negatively regulate TGF-β responses helping balance cellular growth. TRIM33 also interfaces with other pathway regulators like p53 influencing cell cycle and repair mechanisms.

TRIM33 is linked to cancer and fibrosis. Mutations or deregulation of TRIM33 contribute to the development of leukemia where it interacts with proteins like AML1-ETO promoting leukemogenesis through altered transcriptional regulation. In pulmonary fibrosis TRIM33's interaction with SMAD3 illustrates its role in pathological signaling highlighting therapeutic potential in modulating its activity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed : 16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).

See full target information TRIM33

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Cell & bioscience 11:3 PubMed33407858

2021

Retinol from hepatic stellate cells via STRA6 induces lipogenesis on hepatocytes during fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Injoo Hwang,Eun Ju Lee,Hyomin Park,Dodam Moon,Hyo-Soo Kim

The Journal of experimental medicine 217: PubMed32267915

2020

Hepatic stellate cell-specific knockout of transcriptional intermediary factor 1γ aggravates liver fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Eun Ju Lee,Injoo Hwang,Ji Yeon Lee,Jong Nam Park,Keun Cheon Kim,Irene Kim,Dodam Moon,Hyomin Park,Seo-Yeon Lee,Hong Sug Kim,Dae Won Jun,Sung-Hye Park,Hyo-Soo Kim

International journal of rheumatic diseases 22:314-320 PubMed30398003

2018

Anti-TIF1γ antibody and the expression of TIF1γ in idiopathic inflammatory myopathies.

Applications

Unspecified application

Species

Unspecified reactive species

Young Kim,Kyu Sang Song,Eun Hee Sohn,Seong Wook Kang,In Seol Yoo,Seung-Cheol Shim,Su-Jin Yoo,Jinhyun Kim

The Prostate 69:1603-1610 PubMed19644955

2009

Comparison of ACINUS, caspase-3, and TUNEL as apoptotic markers in determination of tumor growth rates of clinically localized prostate cancer using image analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Swaroop S Singh,Diana C Mehedint,O Harris Ford,D Antony Jeyaraj,Elena A Pop,Susan J Maygarden,Anastasia Ivanova,Rameela Chandrasekhar,Gregory E Wilding,James L Mohler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM33 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM33 antibody (AB84455)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

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