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AB300146

Anti-TRIM33 antibody [EPR25102-19]

  • 20ul selling size
  • RabMAb
  • Recombinant
  • KO Validated
  • What is this?

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(5 Publications)

Rabbit Recombinant Monoclonal TRIM33 antibody. Suitable for WB, IP and reacts with Human samples. Cited in 5 publications.

View Alternative Names

KIAA1113, RFG7, TIF1G, TRIM33, E3 ubiquitin-protein ligase TRIM33, Ectodermin homolog, RET-fused gene 7 protein, RING-type E3 ubiquitin transferase TRIM33, Transcription intermediary factor 1-gamma, Tripartite motif-containing protein 33, Protein Rfg7, TIF1-gamma

1 Images
Western blot - Anti-TRIM33 antibody [EPR25102-19] (AB300146)
  • WB

Lab

Western blot - Anti-TRIM33 antibody [EPR25102-19] (AB300146)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Lanes 1-4 : Merged signal (red and green). Green - ab300146 observed at 150 kDa. Red - loading control ab8245 observed at 36 kDa.

ab300146 Anti-TRIM33 antibody [EPR25102-19] was shown to specifically react with TRIM33 in wild-type HAP1 cells. Loss of signal was observed when knockout cell line was used. Wild-type and TRIM33 knockout samples were subjected to SDS-PAGE. ab300146 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at 4℃ overnight at 1 in 1000 dilution and 1 in 20000 dilution respectively.

Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-TRIM33 antibody [EPR25102-19] (ab300146) at 1/1000 dilution

Lane 1:

Wild-type HAP1 whole cell lysate

Lane 2:

TRIM33 knockout HAP1 whole cell lysate

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate

Lane 4:

293T (human embryonic kidney epithelial cell) whole cell lysate

Secondary

Lanes 1 - 4:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW)

Lanes 1 - 4:

Goat Anti-Mouse IgG H&L (IRDye® 680RD)

Observed band size: 150 kDa

false

  • Carrier free

    Anti-TRIM33 antibody [EPR25102-19] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25102-19

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM33 also known as TIF1γ is an E3 ubiquitin ligase involved in protein ubiquitination influencing protein stability and activity. It has a molecular mass of approximately 135 kDa. TRIM33's expression occurs widely in human tissues with higher levels found in the spleen thymus and testis. The protein contains a RING finger domain B-box type zinc fingers and coiled-coil region which are typical for the TRIM family.
Biological function summary

The ubiquitination actions of TRIM33 modulate transcriptional regulation and cell fate decisions. TRIM33 interacts with transcription factors and chromatin often forming part of the larger transcriptional regulatory complexes. Its role in repressing TGF-β signaling by ubiquitylating SMAD proteins highlights its influence on cellular growth and differentiation.

Pathways

TRIM33 has an impact on the TGF-β and BMP signaling pathways both essential for controlling cell proliferation and apoptosis. It acts in partnership with other proteins such as SMAD4 to negatively regulate TGF-β responses helping balance cellular growth. TRIM33 also interfaces with other pathway regulators like p53 influencing cell cycle and repair mechanisms.

TRIM33 is linked to cancer and fibrosis. Mutations or deregulation of TRIM33 contribute to the development of leukemia where it interacts with proteins like AML1-ETO promoting leukemogenesis through altered transcriptional regulation. In pulmonary fibrosis TRIM33's interaction with SMAD3 illustrates its role in pathological signaling highlighting therapeutic potential in modulating its activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed : 16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade).
See full target information TRIM33
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

PloS one 20:e0332761 PubMed41060973

2025

SAT1 promotes the progression of OA by regulating TRIM33-mediated p53 acetylation to enhance ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jiexiang Yang,Jing Jiang,Jing Wang,Lin Luo

International journal of molecular sciences 25: PubMed39062881

2024

Multi-Omics Characterization of E3 Regulatory Patterns in Different Cancer Types.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongyan Li,Jingting Wan,Shangfu Li,Yun Tang,Yang-Chi-Dung Lin,Jie Ni,Xiaoxuan Cai,Jinhan Yu,Hsien-Da Huang,Tzong-Yi Lee

Journal of translational medicine 22:445 PubMed38735939

2024

Multi-omics integration highlights the role of ubiquitination in endometriosis fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Yang,Hong Jiang,Xinyu Ding,Lu Zhang,Huaying Zhang,Jiahao Chen,Lijun Li,Xinqin He,Zhixiong Huang,Qionghua Chen

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2303484 PubMed37946697

2023

Inhibition of CARM1-Mediated Methylation of ACSL4 Promotes Ferroptosis in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Shengjie Feng,Zejun Rao,Jiakun Zhang,Xiaowei She,Yaqi Chen,Kairui Wan,Haijie Li,Chongchong Zhao,Yongdong Feng,Guihua Wang,Junbo Hu,Xuelai Luo

Immunity, inflammation and disease 10:e696 PubMed36301038

2022

KLF9 positively regulates TRIM33 to inhibit abnormal synovial fibroblast proliferation, migration as well as inflammation in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Huang,Liju Tao,Xiuri Du
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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