JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB4527

Anti-TRIM4 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Goat Polyclonal TRIM4 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human TRIM4 aa 450-500.

View Alternative Names

RNF87, TRIM4, E3 ubiquitin-protein ligase TRIM4, RING finger protein 87, RING-type E3 ubiquitin transferase TRIM4, Tripartite motif-containing protein 4

1 Images
Western blot - Anti-TRIM4 antibody (AB4527)
  • WB

Unknown

Western blot - Anti-TRIM4 antibody (AB4527)

ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

ab4527 staining (1ug/ml) of HepG2 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by Western blot using chemiluminescence.

All lanes:

Western blot - Anti-TRIM4 antibody (ab4527)

Predicted band size: 57 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human TRIM4 aa 450-500. The exact immunogen used to generate this antibody is proprietary information.

Q9C037

Specificity

This antibody is expected to recognise both human isoforms.

style
left-column

Complementary Products

Primary Antibodies

AB236422

Anti-TRIM44 antibody [OTI1E10]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

  • 0
  • 0
Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
Primary Antibodies

AB69639

Anti-TRIM59 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM59 antibody (AB69639)

  • 4
  • 1
Primary Antibodies

AB5103

Anti-Histone H3 (citrulline R2 + R8 + R17) antibody

Western blot - Anti-Histone H3 (citrulline R2 + R8 + R17) antibody (AB5103)

  • 4
  • 28
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM4 also known as tripartite motif-containing protein 4 is a protein that functions mechanically as an E3 ubiquitin-protein ligase. It facilitates the attachment of ubiquitin molecules onto specific substrate proteins marking them for proteasomal degradation. TRIM4 has a molecular mass of approximately 57 kDa. It is expressed in various tissues with notable expression in immune system-related tissues such as the spleen and lymph nodes.
Biological function summary

TRIM4 participates in the regulation of innate immune responses and antiviral defense. It is not known to be a component of large multi-protein complexes instead acting more autonomously. The protein helps in modulating the levels of interferon and other cytokines which are important for the body's antiviral response. Its ability to interact with signaling molecules like MAVS influences its role in immune regulation.

Pathways

TRIM4 plays an important role in the immune signaling cascade. It is involved in the RIG-I-like receptor signaling pathway where it helps amplify antiviral signaling in response to pathogen detection. The protein interacts with other molecules in the pathway including RIG-I and TBK1 to promote the production of antiviral cytokines. These interactions establish TRIM4's position as an important player in the maintenance of immune homeostasis during viral infections.

TRIM4 has associations with certain viral infections and autoimmune disorders. Altered regulation or expression of TRIM4 is linked to conditions characterized by dysregulation of immune responses such as systemic lupus erythematosus (SLE). The function of TRIM4 in these conditions can be modulated by interactions with proteins like IRF3 which are critical for the control of inflammatory responses. Understanding TRIM4's role can facilitate the development of therapeutic strategies in targeting immune-related diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

E3 ubiquitin-protein ligase. Mediates 'Lys-63'-linked polyubiquitination of the innate immune receptor RIGI, this linkage doesn't lead to proteasomal degradation but seems to enhance IFN induction.
See full target information TRIM4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 21:3883-95 PubMed22678061

2012

Early transcriptional changes linked to naturally occurring Huntington's disease mutations in neural derivatives of human embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Maxime Feyeux,Fany Bourgois-Rocha,Amanda Redfern,Peter Giles,Nathalie Lefort,Sophie Aubert,Caroline Bonnefond,Aurore Bugi,Marta Ruiz,Nicole Deglon,Lesley Jones,Marc Peschanski,Nicholas D Allen,Anselme L Perrier
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com