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AB236422

Anti-TRIM44 antibody [OTI1E10]

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(14 Publications)

Mouse Monoclonal TRIM44 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Recombinant full length protein - Human, Human, Transfected cell line samples. Cited in 14 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM44.

View Alternative Names

DIPB, TRIM44, Tripartite motif-containing protein 44, Protein DIPB

11 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human liver cancer tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human breast tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human lymphoma tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human tonsil tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human pancreas tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human liver tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human endometrium tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRIM44 antibody [OTI1E10] (AB236422)

COS-7 (human epithelial cell line from cervix adenocarcinoma) cells transiently transfected with pCMV6-ENTRY TRIM44 stained for TRIM44 (green) using ab236422 at 1/100 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human kidney tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM44 antibody [OTI1E10] (AB236422)

Paraffin-embedded human bladder carcinoma tissue stained for TRIM44 using ab236422 at 1/150 dilution in immunohistochemical analysis.

Western blot - Anti-TRIM44 antibody [OTI1E10] (AB236422)
  • WB

Unknown

Western blot - Anti-TRIM44 antibody [OTI1E10] (AB236422)

All lanes:

Western blot - Anti-TRIM44 antibody [OTI1E10] (ab236422) at 1/2000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control cell lysate at 5 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY TRIM44 cell lysate at 5 µg

Predicted band size: 38 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1E10

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRIM44.

Q96DX7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p>Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Clone OTI1E10 (formerly 1E10).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM44 also known as RNF104 is a member of the tripartite motif (TRIM) family of proteins. It has a molecular mass of approximately 67 kDa. TRIM44 is found in various tissues including the lungs liver kidney and muscle. Its mechanical role involves functioning as an E3 ubiquitin ligase facilitating the transfer of ubiquitin to specific protein substrates which tags them for degradation or alters their function and location. The C-terminal RING domain of TRIM44 is important for its ubiquitin ligase activity.
Biological function summary

TRIM44 participates in cell proliferation migration and differentiation. It plays a role in maintaining cellular homeostasis by regulating protein turnover and stability. TRIM44 does not form part of a larger complex but operates independently. By ensuring proper degradation and recycling of proteins it impacts various cellular processes and is linked to cellular stress responses. Its regulatory functions extend to influence various biological processes critical for normal cellular operation.

Pathways

TRIM44 interacts with the ubiquitin-proteasome system and the PI3K/AKT signaling pathway. In the ubiquitin-proteasome system TRIM44 ubiquitinates substrates for proteasomal degradation ensuring cellular protein quality control. Through the PI3K/AKT pathway TRIM44 influences cell survival and growth by modulating signaling proteins within the pathway such as AKT and mTOR. TRIM44's activity within these pathways highlights its role in cellular homeostasis and response to growth signals.

TRIM44 has been implicated in cancer and neurodegenerative diseases. In various cancers such as ovarian and hepatocellular carcinoma TRIM44 overexpression has linked to increased cancer cell proliferation and invasion often through its modulation of the PI3K/AKT pathway. Additionally in neurodegenerative diseases TRIM44 influences protein quality control connecting to proteins involved in proteostasis such as Parkin in Parkinson’s disease. These associations suggest TRIM44's activity correlates with disease progression marking it as a potential target for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

May play a role in the process of differentiation and maturation of neuronal cells (By similarity). May regulate the activity of TRIM17. Is a negative regulator of PAX6 expression (PubMed : 26394807).
See full target information TRIM44
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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Thoracic cancer 16:e70079 PubMed40324967

2025

NAT10 Knockdown Improves Cisplatin Sensitivity in Non-Small Cell Lung Cancer by Inhibiting the TRIM44/PI3K/AKT Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Sun,Xiansong Yang,Ye Wang,Kejia Yang,Yuan Weng

Discover oncology 16:248 PubMed40014271

2025

TRIM44 facilitates aggressive behaviors in multiple myeloma through promoting ZEB1 deubiquitination.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Qi,Jing Wang,Lixia Cao

Evidence-based complementary and alternative medicine : eCAM 2022:6235771 PubMed36387361

2022

TRIM44 Promotes Endometrial Carcinoma Progression by Activating the FRS2 Signalling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yurong Song,Guiju Zhou,Enxue Song,Lei Zhan,Qingyuan Wang,Hui Song,Jingxian Xia,Lin Cong

The Journal of experimental medicine 219: PubMed35616614

2022

MPST sulfurtransferase maintains mitochondrial protein import and cellular bioenergetics to attenuate obesity.

Applications

Unspecified application

Species

Unspecified reactive species

Antonia Katsouda,Dimitrios Valakos,Vasilios S Dionellis,Sofia-Iris Bibli,Ioannis Akoumianakis,Sevasti Karaliota,Karim Zuhra,Ingrid Fleming,Noriyuki Nagahara,Sophia Havaki,Vassilis G Gorgoulis,Dimitris Thanos,Charalambos Antoniades,Csaba Szabo,Andreas Papapetropoulos

Neuroimmunomodulation 29:439-449 PubMed35609523

2022

Silencing of TRIM44 Inhibits Inflammation and Alleviates Traumatic Brain Injury in Rats by Downregulating TLR4-NF-κB Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Ce Dong,Xiongfei Yue,Pengzhen Ge,Guozhen Zheng,Zhanying Ye,Baogen Pan

Translational cancer research 11:414-425 PubMed35281418

2022

Knockdown of inhibits the progression of ovarian cancer and is related to the signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Fanling Meng,Jing Ding,Wei Xu,Chang Luo,Xihai Chen,Ruichun Zhang,Lin Sui,Yuanlong Hu,Shuang Liu,Guangyue Shi,Yunlong He,Xin Ning,Rong Ma,Ning Huang

Genes & genomics 43:1413-1421 PubMed34677810

2021

YTHDF1 promotes the proliferation, migration, and invasion of prostate cancer cells by regulating TRIM44.

Applications

Unspecified application

Species

Unspecified reactive species

Weijian Li,Gaohuang Chen,Zhenyu Feng,Baoyi Zhu,Lilin Zhou,Yuying Zhang,Junyan Mai,Chonghe Jiang,Jianwen Zeng

iScience 24:103271 PubMed34761190

2021

miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sijie Xie,Xiaoxue Jiang,Rushi Qin,Shuting Song,Yanan Lu,Liyan Wang,Yingjie Chen,Dongdong Lu

Neoplasma 68:751-759 PubMed34034495

2021

TRIM44 facilitates ovarian cancer proliferation, migration, and invasion by inhibiting FRK.

Applications

Unspecified application

Species

Unspecified reactive species

Xiu-Zhang Yu,Jia-Ling Yuan,Hui Ye,Ke Yi,Ming-Rong Qie,Min-Min Hou

The Tohoku journal of experimental medicine 254:17-23 PubMed34011804

2021

Tripartite Motif-Containing 44 is Involved in the Tumorigenesis of Laryngeal Squamous Cell Carcinoma, and its Expression is Downregulated by Nuciferine.

Applications

Unspecified application

Species

Unspecified reactive species

Qing-Hua Li,Li-Ping Sui,Yun-Hua Zhao,Bao-Gang Chen,Jian Li,Zhi-Hong Ma,Zhi-Hong Hu,Yan-Li Tang,You-Xin Guo
View all publications
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