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AB169036

Anti-TRIM45 antibody

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(2 Publications)

Mouse Polyclonal TRIM45 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM45.

View Alternative Names

RNF99, TRIM45, E3 ubiquitin-protein ligase TRIM45, RING finger protein 99

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-TRIM45 antibody (AB169036)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRIM45 antibody (AB169036)

Immunofluorescent analysis of permeabilized HeLa cells labeling TRIM45 with ab169036 at 10μg/ml.

Western blot - Anti-TRIM45 antibody (AB169036)
  • WB

Supplier Data

Western blot - Anti-TRIM45 antibody (AB169036)

All lanes:

Western blot - Anti-TRIM45 antibody (ab169036) at 1 µg/mL

Lane 1:

TRIM45-transfected 293T cell line lysate

Lane 2:

Non-transfected 293T cell line lysate

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

Predicted band size: 64 kDa

true

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human TRIM45.

Q9H8W5

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM45 also known as tripartite motif-containing protein 45 belongs to the TRIM/RBCC protein family. It acts mechanically as an E3 ubiquitin-protein ligase facilitating the attachment of ubiquitin to substrate proteins a process vital for proteasomal degradation. TRIM45 has an approximate mass of 55 kDa. Expression of TRIM45 occurs in various tissues particularly in the brain. It contains a RING finger domain one B-box type zinc finger and a coiled-coil region defining its structure.
Biological function summary

TRIM45 functions in the regulation of transcriptional activity and cell signaling pathways. It operates independently and may also form part of a larger protein complex during these activities. TRIM45 plays a role in cellular stress responses and modulates transcription factors through its ubiquitinligase activity thereby influencing gene expression. By controlling these processes TRIM45 impacts cellular proliferation and survival.

Pathways

TRIM45 participates in critical signaling pathways such as the MAPK/ERK pathway and the Wnt signaling pathway. In the MAPK/ERK pathway TRIM45 interacts with various kinases to regulate cell growth and differentiation. In the Wnt signaling pathway TRIM45 associates with proteins like β-catenin to influence cell fate decisions. These connections enable TRIM45 to coordinate cellular responses to environmental stimuli impacting many cellular processes.

TRIM45 has a connection to neurodegenerative diseases and certain cancers. Alterations in TRIM45 expression or function may contribute to the pathogenesis of diseases like glioblastoma. Decreased or dysfunctional TRIM45 can lead to dysregulated cell growth making TRIM45 a potential target for therapeutic intervention. Studies have shown interactions of TRIM45 with proteins involved in these conditions such as APC in cancer progression emphasizing its significance in pathological contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that plays a role in the regulation of inflammatory response (PubMed : 36681779). Mechanistically, mediates the 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, leading to its degradation via the proteasomal pathway and inhibition of the TLR-mediated inflammatory immune response (PubMed : 36681779). May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
See full target information TRIM45
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 20:284 PubMed38037161

2023

TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Xuliang Huang,Changzhou Ye,Xinyu Zhao,Yao Tong,Wen Lin,Qingqing Huang,Yuhao Zheng,Junlu Wang,Anqi Zhang,Yunchang Mo

Oncology reports 47: PubMed34958114

2021

Methylation and mutation of the inhibin‑α gene in human melanoma cells and regulation of PTEN expression and AKT/PI3K signaling by a demethylating agent.

Applications

Unspecified application

Species

Unspecified reactive species

Hyunmin Ko,Hyung Joon Ahn,Young Il Kim
View all publications
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Product promise

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