Mouse Polyclonal TRIM45 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM45.
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Publications
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- Journal of neuroinflammation 20:2842023TRIM45 aggravates microglia pyroptosis via Atg5/NLRP3 axis in septic encephalopathy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuliang Huang,Changzhou Ye,Xinyu Zhao,Yao Tong,Wen Lin,Qingqing Huang,Yuhao Zheng,Junlu Wang,Anqi Zhang,Yunchang MoPubMed 38037161
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human TRIM45. Database link Q9H8W5
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Target data
Function
E3 ubiquitin-protein ligase that plays a role in the regulation of inflammatory response (PubMed:36681779). Mechanistically, mediates the 'Lys-48'-linked polyubiquitination of TAB2, a regulatory protein of the kinase TAK1, leading to its degradation via the proteasomal pathway and inhibition of the TLR-mediated inflammatory immune response (PubMed:36681779). May act as a transcriptional repressor in mitogen-activated protein kinase signaling pathway.
Alternative names
RNF99, TRIM45, E3 ubiquitin-protein ligase TRIM45, RING finger protein 99
Recommended products
Mouse Polyclonal TRIM45 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human TRIM45.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human TRIM45. Database link Q9H8W5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
TRIM45 also known as tripartite motif-containing protein 45 belongs to the TRIM/RBCC protein family. It acts mechanically as an E3 ubiquitin-protein ligase facilitating the attachment of ubiquitin to substrate proteins a process vital for proteasomal degradation. TRIM45 has an approximate mass of 55 kDa. Expression of TRIM45 occurs in various tissues particularly in the brain. It contains a RING finger domain one B-box type zinc finger and a coiled-coil region defining its structure.
- Biological function summary
TRIM45 functions in the regulation of transcriptional activity and cell signaling pathways. It operates independently and may also form part of a larger protein complex during these activities. TRIM45 plays a role in cellular stress responses and modulates transcription factors through its ubiquitinligase activity thereby influencing gene expression. By controlling these processes TRIM45 impacts cellular proliferation and survival.
- Pathways
TRIM45 participates in critical signaling pathways such as the MAPK/ERK pathway and the Wnt signaling pathway. In the MAPK/ERK pathway TRIM45 interacts with various kinases to regulate cell growth and differentiation. In the Wnt signaling pathway TRIM45 associates with proteins like β-catenin to influence cell fate decisions. These connections enable TRIM45 to coordinate cellular responses to environmental stimuli impacting many cellular processes.
- Associated diseases and disorders
TRIM45 has a connection to neurodegenerative diseases and certain cancers. Alterations in TRIM45 expression or function may contribute to the pathogenesis of diseases like glioblastoma. Decreased or dysfunctional TRIM45 can lead to dysregulated cell growth making TRIM45 a potential target for therapeutic intervention. Studies have shown interactions of TRIM45 with proteins involved in these conditions such as APC in cancer progression emphasizing its significance in pathological contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-TRIM45 antibody (ab169036)
Immunofluorescent analysis of permeabilized HeLa cells labeling TRIM45 with ab169036 at 10μg/ml.
Western blot - Anti-TRIM45 antibody (ab169036)
All lanes: Western blot - Anti-TRIM45 antibody (ab169036) at 1 µg/mL
Lane 1: TRIM45-transfected 293T cell line lysate
Lane 2: Non-transfected 293T cell line lysate
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 64 kDa
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