Anti-TRIM46 antibody [EPR26957-34]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TRIM46 with ab307967 at 1/2000 dilution (0.256 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection). Positive staining on the axons of human cerebrum. The section was incubated with ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling TRIM46 with ab307967 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab307967.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of paraffin-embedded human liver  tissue labeling TRIM46 with ab307967 at 1/2000 dilution (0.256 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection). Negative control : No staining on human liver. The section was incubated with ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling TRIM46 with ab307967 at 1/100 dilution (5.12 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Low expression : confocal image showing no staining on mouse heart (PMID : 35440129). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart (fresh) tissue labeling TRIM46 with ab307967 at 1/100 dilution (5.12 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Low expression : confocal image showing no staining on rat heart (PMID : 35440129). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling TRIM46 with ab307967 at 1/100 dilution (5.12 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Low expression : confocal image showing no staining on mouse liver (PMID : 35440129). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling TRIM46 with ab307967 at 1/1000 dilution (0.512 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green). Confocal image showing proximal part of the axon staining in mouse primary neural/glia cell. Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 dilution (4 ug/ml), followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue). -ve control 1 : ab307967 at 1/1000 dilution followed by ab150120 at 1/1000 dilution. -ve control 2 : ab11267 at 1/50 dilution followed by ab150081 at 1/1000 dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cortex (fresh) tissue labeling TRIM46 with ab307967 at 1/100 dilution (5.12 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Panel A : merged staining of anti-TRIM46 (ab307967, green) and anti-NeuN (ab190565, red) on mouse cortex. Panel B : anti-TRIM46 stained on the mouse cortex. Panel C : anti-NeuN stained in neurons of mouse cortex. The section was incubated in two rounds of staining : in the order of ab307967 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized mouse primary neurons labeling TRIM46 with ab307967 at 1/500 dilution (0.1 µg) (Right panel) compared with a Rabbit monoclonal IgG isotype control (ab172730) (Left panel). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab307967.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cortex (fresh) tissue labeling TRIM46 with ab307967 at 1/100 dilution (5.12 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Panel A : merged staining of anti-TRIM46 (ab307967, green) and anti-NeuN (ab190565, red) on rat cortex. Panel B : anti-TRIM46 stained on the rat cortex. Panel C : anti-NeuN stained in neurons of rat cortex. The section was incubated in two rounds of staining : in the order of ab307967 and ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse hippocampus tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, FMRP with ab259335 at 1 : 10000 ( 0.04 µg/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse hippocampus.
Panel B : anti-TRIM46 staining the proximal part of the axon in mouse hippocampus.
Panel C : anti-FMRP staining neurons in mouse hippocampus.
Panel D : anti-Serotonin transporter staining dendrites in mouse hippocampus.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab259335 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRIM46 with ab307967 at 1/5000 dilution (0.102 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection). Positive staining on the axons of mouse cerebrum (PMID : 26671463). The section was incubated with ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling TRIM46 with ab307967 at 1/100 dilution (5.12 µg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green). Low expression : confocal image showing no staining on rat liver (PMID : 35440129). The nuclear counterstain was DAPI (Blue). The section was incubated with ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat spinal cord tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, FMRP with ab259335 at 1 : 10000 (0.04 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat spinal cord.
Panel B : anti-TRIM46 staining the proximal part of the axon in rat spinal cord.
Panel C : anti-FMRP staining neurons in rat spinal cord.
Panel D : anti-Serotonin transporter staining dendrites in rat spinal cord.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab259335 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat hippocampus tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, FMRP with ab259335 at 1 : 10000 (0.04 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat hippocampus.
Panel B : anti-TRIM46 staining the proximal part of the axon in rat hippocampus.
Panel C : anti-FMRP staining neurons in rat hippocampus.
Panel D : anti-Serotonin transporter staining dendrites in rat hippocampus..
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab259335 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TRIM46 with ab307967 at 1/5000 dilution (0.102 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection). Positive staining on the axons of rat cerebrum. The section was incubated with ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection). Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat hippocampus tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, VAChT with ab317452 at 1 : 1000 (0.508 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat hippocampus.Panel B : anti-TRIM46 staining the proximal part of the axon in rat hippocampus.Panel C : anti-VAChT staining vesicle in rat hippocampus.Panel D : anti-Serotonin transporter staining dendrites in rat hippocampus.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab317452 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat cerebrum tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, VAChT with ab317452 at 1 : 1000 (0.508 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat cerebrum.Panel B : anti-TRIM46 staining the proximal part of the axon in rat cerebrum.Panel C : anti-VAChT staining vesicle in rat cerebrum.Panel D : anti-Serotonin transporter staining dendrites in rat cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab317452 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat cerebrum tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, FMRP with ab259335 at 1 : 10000 (0.04 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat cerebrum.
Panel B : anti-TRIM46 staining the proximal part of the axon in rat cerebrum.
Panel C : anti-FMRP staining neurons in rat cerebrum.
Panel D : anti-Serotonin transporter staining dendrites in rat cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab259335 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse hippocampus tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, VAChT with ab317452 at 1 : 1000 (0.508 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse hippocampus.Panel B : anti-TRIM46 staining the proximal part of the axon in mouse hippocampus.Panel C : anti-VAChT staining vesicle in mouse hippocampus.Panel D : anti-Serotonin transporter staining dendrites in mouse hippocampus.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab317452 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cerebrum tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, VAChT with ab317452 at 1 : 1000 (0.508 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse cerebrum.Panel B : anti-TRIM46 staining the proximal part of the axon in mouse cerebrum.Panel C : anti-VAChT staining vesicle in mouse cerebrum.Panel D : anti-Serotonin transporter staining dendrites in mouse cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab317452 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cerebrum tissue staining TRIM46 with ab307967 at a 1 : 5000 (0.102 ug/ml) dilution, FMRP with ab259335 at 1 : 10000 (0.04 ug/ml) dilution and Serotonin transporter with ab308443 at a 1 : 500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

Panel A : merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse cerebrum.
Panel B : anti-TRIM46 staining the proximal part of the axon in mouse cerebrum.
Panel C : anti-FMRP staining neurons in mouse cerebrum.
Panel D : anti-Serotonin transporter staining dendrites in mouse cerebrum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab307967, ab259335 and ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : heart, liver (PMID : 35440129). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 3 minutes.

All lanes:

Western blot - Anti-TRIM46 antibody [EPR26957-34] (ab307967) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 80 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Western blot : Rabbit Monoclonal [EPR26957-34] to TRIM46 ab307967 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta.

A band was observed at 83 kDa in Wild-type A549 cell lysates with no signal observed at this size in TRIM46 knockout A549 cell line.

To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.

Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-TRIM46 antibody [EPR26957-34] (ab307967) at 1/1000 dilution

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

TRIM46 knockout A549 at 20 µg

Lane 3:

U-2 OS at 20 µg

Lane 4:

HT-29 at 20 µg

Secondary

All lanes:

Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

• WB

Supplier Data

Western blot - Anti-TRIM46 antibody [EPR26957-34] (AB307967)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : heart, liver (PMID : 35440129). In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200, 000 dilution. Exposure time : 5 seconds.

All lanes:

Western blot - Anti-TRIM46 antibody [EPR26957-34] (ab307967) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 80 kDa

false

Exposure time: 5s