Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free

24 product images

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cerebrum tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1:10000 (0.04 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse cerebrum.
  Panel B: anti-TRIM46 staining the proximal part of the axon in mouse cerebrum.
  Panel C: anti-FMRP staining neurons in mouse cerebrum.
  Panel D: anti-Serotonin transporter staining dendrites in mouse cerebrum.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-FMRP antibody [EPR23852-90] ab259335 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse hippocampus tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1:10000 ( 0.04 µg/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse hippocampus.
  Panel B: anti-TRIM46 staining the proximal part of the axon in mouse hippocampus.
  Panel C: anti-FMRP staining neurons in mouse hippocampus.
  Panel D: anti-Serotonin transporter staining dendrites in mouse hippocampus.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-FMRP antibody [EPR23852-90] ab259335 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat cerebrum tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1:10000 (0.04 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat cerebrum.
  Panel B: anti-TRIM46 staining the proximal part of the axon in rat cerebrum.
  Panel C: anti-FMRP staining neurons in rat cerebrum.
  Panel D: anti-Serotonin transporter staining dendrites in rat cerebrum.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-FMRP antibody [EPR23852-90] ab259335 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat hippocampus tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1:10000 (0.04 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat hippocampus.
  Panel B: anti-TRIM46 staining the proximal part of the axon in rat hippocampus.
  Panel C: anti-FMRP staining neurons in rat hippocampus.
  Panel D: anti-Serotonin transporter staining dendrites in rat hippocampus..
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-FMRP antibody [EPR23852-90] ab259335 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat spinal cord tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, FMRP with Anti-FMRP antibody [EPR23852-90] ab259335 at 1:10000 (0.04 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-FMRP (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat spinal cord.
  Panel B: anti-TRIM46 staining the proximal part of the axon in rat spinal cord.
  Panel C: anti-FMRP staining neurons in rat spinal cord.
  Panel D: anti-Serotonin transporter staining dendrites in rat spinal cord.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-FMRP antibody [EPR23852-90] ab259335 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse cerebrum tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, VAChT with Anti-VAChT antibody [EPR29154-71] ab317452 at 1:1000 (0.508 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse cerebrum.
  Panel B: anti-TRIM46 staining the proximal part of the axon in mouse cerebrum.
  Panel C: anti-VAChT staining vesicle in mouse cerebrum.
  Panel D: anti-Serotonin transporter staining dendrites in mouse cerebrum.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-VAChT antibody [EPR29154-71] ab317452 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat cerebrum tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, VAChT with Anti-VAChT antibody [EPR29154-71] ab317452 at 1:1000 (0.508 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat cerebrum.
  Panel B: anti-TRIM46 staining the proximal part of the axon in rat cerebrum.
  Panel C: anti-VAChT staining vesicle in rat cerebrum.
  Panel D: anti-Serotonin transporter staining dendrites in rat cerebrum.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-VAChT antibody [EPR29154-71] ab317452 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Rat hippocampus tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, VAChT with Anti-VAChT antibody [EPR29154-71] ab317452 at 1:1000 (0.508 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on rat hippocampus.
  Panel B: anti-TRIM46 staining the proximal part of the axon in rat hippocampus.
  Panel C: anti-VAChT staining vesicle in rat hippocampus.
  Panel D: anti-Serotonin transporter staining dendrites in rat hippocampus.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-VAChT antibody [EPR29154-71] ab317452 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Multiplex immunohistochemistry - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.

  Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded Mouse hippocampus tissue staining TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at a 1:5000 (0.102 ug/ml) dilution, VAChT with Anti-VAChT antibody [EPR29154-71] ab317452 at 1:1000 (0.508 ug/ml) dilution and Serotonin transporter with Anti-Serotonin transporter antibody [EPR26259-61] ab308443 at a 1:500 (0.052 ug/ml) dilution followed by secondary antibody Opal Polymer HRP Ms + Rb.

  Panel A: merged staining of anti-TRIM46 (green; Opal^™520), anti-VAChT (magenta; Opal^™690) and anti-Serotonin transporter (yellow; Opal^™570) on mouse hippocampus.
  Panel B: anti-TRIM46 staining the proximal part of the axon in mouse hippocampus.
  Panel C: anti-VAChT staining vesicle in mouse hippocampus.
  Panel D: anti-Serotonin transporter staining dendrites in mouse hippocampus.
  Nuclear DNA was labeled with DAPI (shown in blue).

  The section was incubated in three rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967, Anti-VAChT antibody [EPR29154-71] ab317452 and Anti-Serotonin transporter antibody [EPR26259-61] ab308443 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

  The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal^™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• 

  Western blot - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using 307967, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Negative control: heart, liver (PMID: 35440129).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.
  
  Exposure time: 3 minutes.

  All lanes: Western blot - Anti-TRIM46 antibody [EPR26957-34] (Anti-TRIM46 antibody [EPR26957-34] ab307967) at 1/1000 dilution

  Lane 1: Human brain tissue lysate at 20 µg

  Lane 2: Human liver tissue lysate at 20 µg

  Lane 3: Human heart tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 80 kDa

  Exposure time: 3min

• 

  Western blot - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using 307967, the same antibody clone in a different buffer formulation.
  
  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Negative control: heart, liver (PMID: 35440129).
  
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200, 000 dilution.
  
  Exposure time: 5 seconds.

  All lanes: Western blot - Anti-TRIM46 antibody [EPR26957-34] (Anti-TRIM46 antibody [EPR26957-34] ab307967) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse heart tissue lysate at 20 µg

  Lane 3: Mouse liver tissue lysate at 20 µg

  Lane 4: Rat brain tissue lysate at 20 µg

  Lane 5: Rat heart tissue lysate at 20 µg

  Lane 6: Rat liver tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

  Observed band size: 80 kDa

  Exposure time: 5s

• 

  Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat heart (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Low expression: confocal image showing no staining on rat heart (PMID: 35440129).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Low expression: confocal image showing no staining on mouse liver (PMID: 35440129).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Low expression: confocal image showing no staining on rat liver (PMID: 35440129).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cortex (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Panel A: merged staining of anti-TRIM46 (Anti-TRIM46 antibody [EPR26957-34] ab307967, green) and anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, red) on mouse cortex.
  Panel B: anti-TRIM46 stained on the mouse cortex.
  Panel C: anti-NeuN stained in neurons of mouse cortex.
  The section was incubated in two rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cortex (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Panel A: merged staining of anti-TRIM46 (Anti-TRIM46 antibody [EPR26957-34] ab307967, green) and anti-NeuN (Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565, red) on rat cortex.
  Panel B: anti-TRIM46 stained on the rat cortex.
  Panel C: anti-NeuN stained in neurons of rat cortex.
  The section was incubated in two rounds of staining: in the order of Anti-TRIM46 antibody [EPR26957-34] ab307967 and Alexa Fluor® 647 Anti-NeuN antibody [EPR12763] - Neuronal Marker ab190565 for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• 

  Immunohistochemistry (Frozen sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse heart (fresh) tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/100 dilution (5.12 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (2 µg/mL) (Green).
  Low expression: confocal image showing no staining on mouse heart (PMID: 35440129).
  The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor488) preadsorbed at 1/1000 dilution (2 µg/mL) dilution.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  
  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neural/glia cells labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/1000 dilution (0.512 µg/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (2 ug/ml) (Green).
  Confocal image showing proximal part of the axon staining in mouse primary neural/glia cell.
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Anti-MAP2 antibody [HM-2] ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 dilution (4 ug/ml), followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 ug/ml) (Red). The nuclear counterstain was DAPI (Blue).
  -ve control 1: Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/1000 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 at 1/1000 dilution.
  -ve control 2: Anti-MAP2 antibody [HM-2] ab11267 at 1/50 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 at 1/1000 dilution.

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  Flow Cytometry (Intracellular) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  
  Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized mouse primary neurons labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/500 dilution (0.1 µg) (Right panel) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Left panel). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-TRIM46 antibody [EPR26957-34] ab307967.

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  Flow Cytometry (Intracellular) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  
  Flow cytometric analysis of 4% paraformaldehyde-fixed 90% methanol-permeabilized SH-SY5Y (human neuroblastoma epithelial cell) cells labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/500 dilution (0.1 µg) (Red) compared with a Rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or Anti-TRIM46 antibody [EPR26957-34] ab307967.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded human liver  tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/2000 dilution (0.256 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  
  Negative control: No staining on human liver.
  
  The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/5000 dilution (0.102 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  
  Positive staining on the axons of rat cerebrum.
  
  The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/5000 dilution (0.102 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  
  Positive staining on the axons of mouse cerebrum (PMID: 26671463).
  
  The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM46 antibody [EPR26957-34] - BSA and Azide free (ab307968)

  This data was developed using Anti-TRIM46 antibody [EPR26957-34] ab307967, the same antibody clone in a different buffer formulation.
  
  Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling TRIM46 with Anti-TRIM46 antibody [EPR26957-34] ab307967 at 1/2000 dilution (0.256 µg/ml) followed by ready to use LeicaDS9800 (Bond ™ Polymer Refine Detection).
  
  Positive staining on the axons of human cerebrum.
  
  The section was incubated with Anti-TRIM46 antibody [EPR26957-34] ab307967 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
  
  Secondary antibody only control: Secondary antibody is ready to use LeicaDS9800 (Bond ™Polymer Refine Detection).
  
  Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.