AB72234

Anti-TRIM47 antibody

Name: Anti-TRIM47 antibody (ab72234) | Abcam
Brand: Abcam Limited
SKU: AB72234
Price: 535 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal TRIM47 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human TRIM47.

View Alternative Names

GOA, RNF100, TRIM47, E3 ubiquitin-protein ligase TRIM47, Gene overexpressed in astrocytoma protein, RING finger protein 100, Tripartite motif-containing protein 47

2 Images

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Immunoprecipitation - Anti-TRIM47 antibody (AB72234)

Immunoprecipitation/ Western Blot of TRIM47.
Lane 1 : ab72234 at 3μg/mg whole cell lysate.
Lane 2 : Control IgG.

ab72234 at 1μg/ml for WB.
Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded.
Chemiluminescence with an exposure time of 3 seconds.

All lanes:

Immunoprecipitation - Anti-TRIM47 antibody (ab72234)

Predicted band size: 70 kDa

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Western blot - Anti-TRIM47 antibody (AB72234)

All lanes:

Western blot - Anti-TRIM47 antibody (ab72234) at 0.04 µg/mL

Lane 1:

Whole cell lysate from Hela cells at 50 µg

Lane 2:

Whole cell lysate from Hela cells at 15 µg

Lane 3:

Whole cell lysate from Hela cells at 5 µg

Lane 4:

Whole cell lysate from 293T cells at 50 µg

Predicted band size: 70 kDa

Observed band size: 130 kDa,70 kDa,80 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human TRIM47. The exact immunogen used to generate this antibody is proprietary information.

Q96LD4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM47 also known as Tripartite Motif Containing 47 or GOF functions as an E3 ubiquitin ligase. This protein has a molecular mass of approximately 67 kDa. It participates in the process of transferring ubiquitin from E2 conjugating enzyme to substrate proteins which leads to proteasomal degradation of these proteins. TRIM47 is expressed in various tissues with high levels observed in the testis and brain and it shows differential expression in certain types of cancers.

Biological function summary

TRIM47 affects cellular processes by regulating protein stability and turnover. As an E3 ubiquitin ligase it assists in tagging proteins for degradation which is important for maintaining protein homeostasis within the cell. By controlling protein levels TRIM47 influences cellular proliferation differentiation and apoptosis. It is involved in the degradation of proteins that are part of cellular signaling pathways which can impact the cell's response to external stimuli and stress.

Pathways

Multiple cellular responses and regulatory networks are influenced by TRIM47 activity. It plays a role in the ubiquitin-proteasome pathway and is involved in the regulation of NF-kB signaling pathways. This interconnection guides how cells react during inflammation and immune responses. TRIM47 interacts with proteins such as IκBα which further allows it to modulate the NF-kB pathway therefore indirectly affecting genes involved in inflammation and immune cell survival.

TRIM47 expression levels correlate with certain cancers including glioblastoma and breast cancer. Dysregulation of TRIM47 can lead to increased degradation of tumor-suppressor proteins promoting tumor growth and progression. The protein's role in cancer links it to other proteins involved in cancer pathways such as p53 and PTEN which are known for their functions in regulating cell cycle and apoptosis. Understanding TRIM47's interactions with these proteins can reveal new therapeutic targets for cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase that mediates the ubiquitination and proteasomal degradation of CYLD.

See full target information TRIM47

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

American journal of reproductive immunology (New York, N.Y. : 1989) 93:e70102 PubMed40435048

2025

Knockdown of TRIM47 Overcomes Paclitaxel Resistance in Ovarian Cancer by Suppressing the TGF-β Pathway via PPM1A.

Applications

Unspecified application

Species

Unspecified reactive species

Hezhu Wang,Shengjun Chen,Feifei Xu,Xiaojing Chen

Translational andrology and urology 13:1991-2004 PubMed39434735

2024

TRIM47 promotes the Warburg effect and reduces ferroptosis in prostate cancer by FBP1 and FOXO1.

Applications

Unspecified application

Species

Unspecified reactive species

Chubiao Zhao,Zengqin Liu,Junming Peng,Jiansheng Huang,Jinan Guo

Cell biology international 48:1138-1147 PubMed38769645

2024

TRIM47 promotes HDM-induced bronchial epithelial pyroptosis by regulating NEMO ubiquitination to activate NF-κB/NLRP3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjuan Zhan,Huifang Zhang,Yufei Su,Li Yin

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 36:e22345 PubMed35635715

2022

HMGN3 represses transcription of epithelial regulators to promote migration of cholangiocarcinoma in a SNAI2-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Supannika Sorin,Sho Kubota,Sofiane Hamidi,Takako Yokomizo-Nakano,Kulthida Vaeteewoottacharn,Sopit Wongkham,Sakda Waraasawapati,Chawalit Pairojkul,Jie Bai,Mariko Morii,Guojun Sheng,Kanlayanee Sawanyawisuth,Goro Sashida

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM47 antibody

Immunoprecipitation - Anti-TRIM47 antibody (AB72234)

Western blot - Anti-TRIM47 antibody (AB72234)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise