AB69639

Anti-TRIM59 antibody

Name: Anti-TRIM59 antibody (ab69639) | Abcam
Brand: Abcam Limited
SKU: AB69639
Price: 545 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(14 Publications)

Rabbit Polyclonal TRIM59 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 14 publications.

View Alternative Names

RNF104, TRIM57, TSBF1, TRIM59, Tripartite motif-containing protein 59, RING finger protein 104, Tumor suppressor TSBF-1

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM59 antibody (AB69639)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM59 antibody (AB69639)

This image shows human brain tissue stained with ab69639 at 1/50 dilution, with and without the immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-TRIM59 antibody (AB69639)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-TRIM59 antibody (AB69639)

This image shows HeLa cells stained with ab69639 at 1/500 dilution, with and without the immunising peptide.

Unknown

Western blot - Anti-TRIM59 antibody (AB69639)

All lanes:

Western blot - Anti-TRIM59 antibody (ab69639) at 1/500 dilution

Lane 1:

extracts from NIH-3T3 cells at 5 µg

Lane 2:

extracts from NIH-3T3 cells at 5 µg with immunising peptide

Predicted band size: 47 kDa

Observed band size: 47 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM59 also known as tripartite motif-containing protein 59 is a protein that weighs approximately 54 kDa. It belongs to the TRIM family and has a conserved RING finger domain which implies a role in ubiquitination. The protein shows expression in various tissues including the liver kidney and spleen. TRIM59 has an E3 ubiquitin ligase activity that adds ubiquitin molecules to target proteins tagging them for degradation by the proteasome. This process helps regulate protein levels and maintain cellular homeostasis.

Biological function summary

TRIM59 influences cellular processes such as proliferation apoptosis and immune response. It acts as part of a complex that participates in protein degradation and signal transduction. The protein affects cell signaling pathways by modulating the ubiquitination and turnover of key signaling molecules. Through these actions TRIM59 can influence how cells respond to external stimuli and manage their internal functions. Alterations in its activity can lead to disruptions in these processes.

Pathways

TRIM59 plays a role in the PI3K/AKT and Wnt signaling pathways. In the PI3K/AKT pathway it affects cellular survival and growth while in the Wnt signaling pathway it influences cell fate determination. TRIM59 interacts with proteins such as AKT and β-catenin which serve as important players in these pathways. These interactions enable TRIM59 to impact downstream signaling events and modulate cellular outcomes contributing to multiple physiological functions and processes.

TRIM59 has a connection with several cancers including prostate cancer and gastric cancer. Elevated levels of TRIM59 often correlate with tumor progression and poor prognosis. In prostate cancer TRIM59 interacts with the androgen receptor influencing its stability and activity. In gastric cancer it affects proteins such as p53 a well-known tumor suppressor leading to altered cell cycle regulation. The dysregulation of TRIM59 and its related pathways suggest potential targeting strategies for therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase involved in different processes such as development and immune response (PubMed : 22588174, PubMed : 30231667). Serves as a negative regulator for innate immune signaling pathways by suppressing RLR-induced activation of IRF3/7 and NF-kappa-B via interaction with adapter ECSIT (PubMed : 22588174). Regulates autophagy through modulating both the transcription and the ubiquitination of BECN1 (PubMed : 30231667). On the one hand, regulates the transcription of BECN1 through negatively modulating the NF-kappa-B pathway. On the other hand, regulates TRAF6-mediated 'Lys-63'-linked ubiquitination of BECN1, thus affecting the formation of the BECN1-PIK3C3 complex. In addition, mediates 'Lys-48'-linked ubiquitination of TRAF6 and thereby promotes TRAF6 proteasomal degradation (PubMed : 30231667). Acts also as a critical regulator for early embryo development from blastocyst stage to gastrula through modulating F-actin assembly and WASH1 'Lys-63'-linked ubiquitination (By similarity).

See full target information TRIM59

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:41 PubMed40238115

2025

TUT7-Mediated Uridine Degradation of MCPIP1 in the Pterygium to Regulate TRAF6-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Juanjuan Li,Hao Ji,Yanze Xu,Weijia Zhang,Yuru Yin,Yubing Zhao,Yan Du,Anni He,Dandan Zhao

Scientific reports 14:2511 PubMed38291200

2024

TRIM59 suppresses the brain ischaemia/reperfusion injury and pyroptosis of microglial through mediating the ubiquitination of NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Liangtian Zhang,Gang Li,Ying Li

Journal of biochemical and molecular toxicology 37:e23473 PubMed37545326

2023

Circ_0006324 regulates cell proliferation, cell-cycle progression, apoptosis, and glycolysis of non-small cell lung cancer cells through miR-496/TRIM59 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Wang,Xu Zhu,Kai Wang,Ronghai Ding

Annals of translational medicine 11:42 PubMed36819529

2023

TRIM59 attenuates ox-LDL-induced endothelial cell inflammation, apoptosis, and monocyte adhesion through AnxA2.

Applications

Unspecified application

Species

Unspecified reactive species

Qilin Zeng,Jingli Xie,Fang Li

Molecular therapy : the journal of the American Society of Gene Therapy 31:774-787 PubMed36523164

2022

SARS-CoV-2 viral protein ORF3A injures renal tubules by interacting with TRIM59 to induce STAT3 activation.

Applications

Unspecified application

Species

Unspecified reactive species

Hong Cai,Ya Chen,Ye Feng,Morad Asadi,Lewis Kaufman,Kyung Lee,Thomas Kehrer,Lisa Miorin,Adolfo Garcia-Sastre,G Luca Gusella,Leyi Gu,Zhaohui Ni,Shan Mou,John Cijiang He,Weibin Zhou

Oncology letters 21:6 PubMed33240412

2020

Blockade of TRIM59 enhances esophageal cancer cell chemosensitivity to cisplatin by upregulating p53.

Applications

Unspecified application

Species

Unspecified reactive species

Rongfeng Liu,Hongchen Li,Yanzhao Xu,Xing Li,Xiaojin Guo,Jian Shi,Yanzhi Cui,Zhiyu Wang,Junfeng Liu

FEBS open bio : PubMed32902187

2020

Histone H3K9 and H3K14 acetylation at the promoter of the LGALS9 gene is associated with mRNA levels in cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Erick Armenta-Castro,Tania Reyes-Vallejo,Daniel Máximo-Sánchez,Irma Herrera-Camacho,Gustavo López-López,Sandra Reyes-Carmona,Ileana Conde-Rodríguez,Ivonne Ramírez-Díaz,Adriana Aguilar-Lemarroy,Luis Felipe Jave-Suárez,Lorena Milflores-Flores,Gerardo Santos-Lopez,Julio Reyes-Leyva,Verónica Vallejo-Ruiz

EMBO reports 21:e49689 PubMed32790025

2020

Epstein-Barr virus-derived circular RNA LMP2A induces stemness in EBV-associated gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li-Ping Gong,Jian-Ning Chen,Min Dong,Zhen-Dong Xiao,Zhi-Ying Feng,Yu-Hang Pan,Yu Zhang,Yu Du,Jing-Yue Zhang,Yuan-Hua Bi,Jun-Ting Huang,Jing Liang,Chun-Kui Shao

Journal of receptor and signal transduction research 40:471-478 PubMed32340525

2020

Tripartite motif containing 59 (TRIM59) promotes esophageal cancer progression via promoting MST4 expression and ERK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guangming Liu,Jinying Song,Yong Zhao,Lianjie Zhang,Junjie Qin,Youbin Cui

Nature communications 10:4013 PubMed31488827

2019

CDK5-dependent phosphorylation and nuclear translocation of TRIM59 promotes macroH2A1 ubiquitination and tumorigenicity.

Applications

Unspecified application

Species

Unspecified reactive species

Youzhou Sang,Yanxin Li,Yingwen Zhang,Angel A Alvarez,Bo Yu,Weiwei Zhang,Bo Hu,Shi-Yuan Cheng,Haizhong Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-TRIM59 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM59 antibody (AB69639)

Immunocytochemistry/ Immunofluorescence - Anti-TRIM59 antibody (AB69639)

Western blot - Anti-TRIM59 antibody (AB69639)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (14)

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