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AB155674

Anti-TRIM8 antibody

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(5 Publications)

Rabbit Polyclonal TRIM8 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM8 aa 1-250.

View Alternative Names

GERP, RNF27, TRIM8, E3 ubiquitin-protein ligase TRIM8, Glioblastoma-expressed RING finger protein, RING finger protein 27, RING-type E3 ubiquitin transferase TRIM8, Tripartite motif-containing protein 8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM8 antibody (AB155674)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM8 antibody (AB155674)

Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labeling TRIM8 with ab155674 at 1/500 dilution.

Western blot - Anti-TRIM8 antibody (AB155674)
  • WB

Unknown

Western blot - Anti-TRIM8 antibody (AB155674)

7.5% SDS PAGE

All lanes:

Western blot - Anti-TRIM8 antibody (ab155674) at 1/1000 dilution

All lanes:

HepG2 whole cell lysate at 30 µg

Predicted band size: 61 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human TRIM8 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q9BZR9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM8 also known as Tripartite Motif Containing 8 is an E3 ubiquitin ligase with a known mass of about 61 kDa. This protein facilitates protein degradation via the ubiquitin-proteasome system. It localizes to the nucleus and cytoplasm and is expressed in various tissues such as the brain and immune cells. TRIM8 influences multiple cellular processes by adding ubiquitin chains to specific proteins thereby tagging them for degradation.
Biological function summary

TRIM8 is involved in the regulation of signaling pathways and acts as a modulator of cellular functions. TRIM8 participates in the stability and turnover of p53 and STAT3 affecting their transcriptional activities. It also contributes to immune responses by modulating pathways that involve interferon signaling. TRIM8 is not typically part of large protein complexes; its interactions often involve single protein-protein connections.

Pathways

TRIM8 plays significant roles in immune signaling and apoptosis regulation. It interacts with the NF-kB pathway influencing inflammation and immune responses. Through this pathway TRIM8 impacts the activation of transcriptional activity affecting cellular survival and proliferation. Additionally TRIM8 interacts with proteins like p53 altering its function in the apoptotic pathway thereby influencing cell cycle regulation and survival.

TRIM8 has connections to cancer and neurodegenerative diseases. For cancer abnormalities in TRIM8 expression levels can result in altered p53 activity promoting tumor growth or suppression. In neurodegenerative contexts TRIM8's interaction with STAT3 can affect inflammatory responses linked to diseases like Alzheimer's. These relationships highlight the importance of TRIM8 in both the progression and inhibition of certain pathologies through its interactions with proteins such as p53 and STAT3.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response (PubMed : 27981609, PubMed : 28747347). Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1 (PubMed : 12163497). Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B (PubMed : 22084099, PubMed : 23152791, PubMed : 27981609, PubMed : 34871740). Also modulates STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus (PubMed : 20516148). Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction (PubMed : 28747347).
See full target information TRIM8
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

The Turkish journal of gastroenterology : the official journal of Turkish Society of Gastroenterology 35:61-72 PubMed38454278

2024

Identification of Novel Prognostic Biomarkers for Colorectal Cancer by Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Niu,Xiaogang Li,Xian Lei Luo,Hongwei Wan,Wendi Jin,Zhiping Zhang,Wanfu Zhang,Bo Li

International journal of molecular sciences 24: PubMed37686095

2023

Identification of Key Genes Involved in Response to or spp. Infections in Human Cell Lines and in Mouse Organs.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina Stepanenko,Natalia Bondareva,Anna Sheremet,Elena Fedina,Alexei Tikhomirov,Tatiana Gerasimova,Daniil Poberezhniy,Irina Makarova,Vyacheslav Tarantul,Nailya Zigangirova,Valentina Nenasheva

Allergologia et immunopathologia 51:92-97 PubMed36617827

2023

Knockdown of TRIM8 alleviates dextran sulfate sodium-induced colitis in mice by inhibiting the NF-κB signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Qiu,Yifei Lv,Lu Niu,Yu Zhang

Frontiers in oncology 12:1002040 PubMed36353542

2022

Identification and validation of novel biomarker TRIM8 related to cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhang,Youli Dan,Chaoyang Ou,Hongyan Qian,Yi Yin,Min Tang,Qian He,Chen Peng,Aiqin He

Molecular psychiatry 26:3444-3460 PubMed32929213

2020

Transcription factor POU3F2 regulates TRIM8 expression contributing to cellular functions implicated in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Chaodong Ding,Chunling Zhang,Richard Kopp,Liz Kuney,Qingtuan Meng,Le Wang,Yan Xia,Yi Jiang,Rujia Dai,Shishi Min,Wei-Dong Yao,Ma-Li Wong,Hongyu Ruan,Chunyu Liu,Chao Chen
View all publications
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Product promise

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