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Rabbit Recombinant Monoclonal TRIM8 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IP and reacts with Human, Mouse, Rat samples.

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Images

Immunoprecipitation - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (AB316150), expandable thumbnail
  • Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (AB316150), expandable thumbnail
  • Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (AB316150), expandable thumbnail
  • Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (AB316150), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (AB316150), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow Cyt (Intra)WBIPIHC-PICC/IF
Human
Tested
Tested
Tested
Not recommended
Not recommended
Mouse
Expected
Tested
Expected
Not recommended
Not recommended
Rat
Expected
Tested
Expected
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

E3 ubiquitin-protein ligase that participates in multiple biological processes including cell survival, differentiation, apoptosis, and in particular, the innate immune response (PubMed:27981609, PubMed:28747347). Participates in the activation of interferon-gamma signaling by promoting proteasomal degradation of the repressor SOCS1 (PubMed:12163497). Plays a positive role in the TNFalpha and IL-1beta signaling pathways. Mechanistically, induces the 'Lys-63'-linked polyubiquitination of MAP3K7/TAK1 component leading to the activation of NF-kappa-B (PubMed:22084099, PubMed:23152791, PubMed:27981609, PubMed:34871740). Modulates also STAT3 activity through negative regulation of PIAS3, either by degradation of PIAS3 through the ubiquitin-proteasome pathway or exclusion of PIAS3 from the nucleus (PubMed:20516148). Negatively regulates TLR3/4-mediated innate immune response by catalyzing 'Lys-6'- and 'Lys-33'-linked polyubiquitination of TICAM1 and thereby disrupting the TICAM1-TBK1 interaction (PubMed:28747347).

Alternative names

GERP, RNF27, TRIM8, E3 ubiquitin-protein ligase TRIM8, Glioblastoma-expressed RING finger protein, RING finger protein 27, RING-type E3 ubiquitin transferase TRIM8, Tripartite motif-containing protein 8

Recommended products

Rabbit Recombinant Monoclonal TRIM8 antibody. Carrier free. Suitable for Flow Cyt (Intra), WB, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR27965-6
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab316150 is the carrirer-free version of Anti-TRIM8 antibody [EPR27965-6] ab316149.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

TRIM8 also known as Tripartite Motif Containing 8 is an E3 ubiquitin ligase with a known mass of about 61 kDa. This protein facilitates protein degradation via the ubiquitin-proteasome system. It localizes to the nucleus and cytoplasm and is expressed in various tissues such as the brain and immune cells. TRIM8 influences multiple cellular processes by adding ubiquitin chains to specific proteins thereby tagging them for degradation.

Biological function summary

TRIM8 is involved in the regulation of signaling pathways and acts as a modulator of cellular functions. TRIM8 participates in the stability and turnover of p53 and STAT3 affecting their transcriptional activities. It also contributes to immune responses by modulating pathways that involve interferon signaling. TRIM8 is not typically part of large protein complexes; its interactions often involve single protein-protein connections.

Pathways

TRIM8 plays significant roles in immune signaling and apoptosis regulation. It interacts with the NF-kB pathway influencing inflammation and immune responses. Through this pathway TRIM8 impacts the activation of transcriptional activity affecting cellular survival and proliferation. Additionally TRIM8 interacts with proteins like p53 altering its function in the apoptotic pathway thereby influencing cell cycle regulation and survival.

Associated diseases and disorders

TRIM8 has connections to cancer and neurodegenerative diseases. For cancer abnormalities in TRIM8 expression levels can result in altered p53 activity promoting tumor growth or suppression. In neurodegenerative contexts TRIM8's interaction with STAT3 can affect inflammatory responses linked to diseases like Alzheimer's. These relationships highlight the importance of TRIM8 in both the progression and inhibition of certain pathologies through its interactions with proteins such as p53 and STAT3.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150), expandable thumbnail

    Immunoprecipitation - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150)

    This data was developed using Anti-TRIM8 antibody [EPR27965-6] ab316149, the same antibody clone in a different buffer formulation.

    TRIM8 was immunoprecipitated from 0.35 mg HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate with Anti-TRIM8 antibody [EPR27965-6] ab316149 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-TRIM8 antibody [EPR27965-6] ab316149 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate

    Lane 2: Anti-TRIM8 antibody [EPR27965-6] ab316149 IP in HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-TRIM8 antibody [EPR27965-6] ab316149 in HCT 116 whole cell lysate

    All lanes: Immunoprecipitation - Anti-TRIM8 antibody [EPR27965-6] (Anti-TRIM8 antibody [EPR27965-6] ab316149) at 1/30 dilution

    All lanes: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 102s

  • Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150), expandable thumbnail

    Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150)

    This data was developed using Anti-TRIM8 antibody [EPR27965-6] ab316149, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-TRIM8 antibody [EPR27965-6] (Anti-TRIM8 antibody [EPR27965-6] ab316149) at 1/1000 dilution

    Lane 1: Mouse brain lysate at 20 µg with 5% NFDM/TBST

    Lane 2: Rat brain lysate at 20 µg with 5% NFDM/TBST

    Lane 3: Rat spleen lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 61 kDa

    Exposure time: 180s

  • Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150), expandable thumbnail

    Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150)

    This data was developed using Anti-TRIM8 antibody [EPR27965-6] ab316149, the same antibody clone in a different buffer formulation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-TRIM8 antibody [EPR27965-6] (Anti-TRIM8 antibody [EPR27965-6] ab316149) at 1/1000 dilution

    Lane 1: HCT 116 (human colorectal carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: HCT 116 transfected with siRNA specifically targeting TRIM8 whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 61 kDa, 36 kDa

    Exposure time: 92s

  • Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150), expandable thumbnail

    Western blot - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150)

    This data was developed using Anti-TRIM8 antibody [EPR27965-6] ab316149, the same antibody clone in a different buffer formulation.

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    The identity of the higher MW band at approximately 90 kDa (in lane 4) is unknown.

    All lanes: Western blot - Anti-TRIM8 antibody [EPR27965-6] (Anti-TRIM8 antibody [EPR27965-6] ab316149) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 5: MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lanes 6 - 7: T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 61 kDa

    Exposure time: 180s

  • Flow Cytometry (Intracellular) - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-TRIM8 antibody [EPR27965-6] - BSA and Azide free (ab316150)

    This data was developed using Anti-TRIM8 antibody [EPR27965-6] ab316149, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HCT 116 (human colorectal carcinoma epithelial cell) cells labelling TRIM8 with Anti-TRIM8 antibody [EPR27965-6] ab316149 at 1/50 dilution (1 ug)/(Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

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Product protocols

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