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AB300516

Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free

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Rabbit Recombinant Monoclonal TRIM9 antibody. Carrier free. Suitable for IP, IHC-P, WB and reacts with Mouse, Human, Rat samples.

View Alternative Names

KIAA0282, RNF91, TRIM9, E3 ubiquitin-protein ligase TRIM9, RING finger protein 91, RING-type E3 ubiquitin transferase TRIM9, Tripartite motif-containing protein 9

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

This data was developed using ab300515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling TRIM9 with ab300515 at 1/5000 (0.095 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cerebrum (PMID : 20085810). The section was incubated with ab300515 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

This data was developed using ab300515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling TRIM9 with ab300515 at 1/5000 (0.095 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse spleen (PMID : 20085810). The section was incubated with ab300515 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

This data was developed using ab300515, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling TRIM9 with ab300515 at 1/5000 (0.095 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : no staining on mouse liver (PMID : 20085810). The section was incubated with ab300515 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • WB

Supplier Data

Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

This data was developed using ab300515, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : human liver tissue (PMID : 20085810).

The antibody detects isoform of TRIM9 around 61kDa which consistent with what has been described in the literature (PMID : 20085810).

The identity of the lower MW band at approximately 37 kDa (in lane 1, 2) is unknown.

The blot of lane 5 was developed using a high sensitivity ECL substrate.

Exposure time : Lane 1-4 : 136 seconds Lane 5 : 3 minutes

All lanes:

Western blot - Anti-TRIM9 antibody [EPR25103-71] (<a href='/en-us/products/primary-antibodies/trim9-antibody-epr25103-71-ab300515'>ab300515</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 40 µg

Lane 3:

IMR-32 (human neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 4:

SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate at 20 µg

Lane 5:

Y79 (human retinoblastoma retinoblastoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 79 kDa

Observed band size: 61 kDa,79 kDa

false

Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • WB

Supplier Data

Western blot - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the bands between 37 kDa and 25 kDa are unknown.

Exposure time : Lane 1-5 : 81 seconds Lane 6, 7 : 52 minutes

All lanes:

Western blot - Anti-TRIM9 antibody [EPR25103-71] (<a href='/en-us/products/primary-antibodies/trim9-antibody-epr25103-71-ab300515'>ab300515</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse hippocampus tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 40 µg

Lane 4:

Mouse cerebellum tissue lysate at 20 µg

Lane 5:

Rat cerebellum tissue lysate at 20 µg

Lane 6:

Rat brain tissue lysate at 20 µg

Lane 7:

Rat liver tissue lysate at 40 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 79 kDa

Observed band size: 61 kDa,79 kDa

false

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • IP

Supplier Data

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

This data was developed using ab300516, the same antibody clone in a different buffer formulation. TRIM9 was immunoprecipitated from 0.35 mg Mouse brain tissue lysate 10 ug with ab300515 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300515 at ID188 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Mouse brain tissue lysate 10 ug Lane 2 : ab300515 IP in Mouse brain tissue lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300515 in mouse brain tissue lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 41 seconds

Lane 1:

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] (<a href='/en-us/products/primary-antibodies/trim9-antibody-epr25103-71-ab300515'>ab300515</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] (<a href='/en-us/products/primary-antibodies/trim9-antibody-epr25103-71-ab300515'>ab300515</a>) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 41s

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)
  • IP

Supplier Data

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] - BSA and Azide free (AB300516)

This data was developed using ab300516, the same antibody clone in a different buffer formulation. TRIM9 was immunoprecipitated from 0.35 mg SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 10 ug with ab300515 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300515 at ID188 dilution. VeriBlot for IP secondary antibody (HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : SK-N-BE(2) (human neuroblastoma neuroblast), whole cell lysate 10 ug Lane 2 : ab300515 IP in SK-N-BE(2) whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab300515 in SK-N-BE(2) whole cell lysate. Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 23 seconds

Lane 1:

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] (<a href='/en-us/products/primary-antibodies/trim9-antibody-epr25103-71-ab300515'>ab300515</a>) at 1/30 dilution

Lane 2:

Immunoprecipitation - Anti-TRIM9 antibody [EPR25103-71] (<a href='/en-us/products/primary-antibodies/trim9-antibody-epr25103-71-ab300515'>ab300515</a>) at 1/1000 dilution

All lanes:

SK-N-BE(2) whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 23s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25103-71

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

TRIM9 also known as Tripartite Motif Containing 9 is an E3 ubiquitin ligase with a mass of approximately 68 kDa. This protein adds ubiquitin to substrate proteins tagging them for proteasomal degradation or altering their activity. TRIM9 contains a RING domain B-box type zinc finger and coiled-coil region typical of the TRIM family proteins. It is widely expressed in the nervous system with high levels found in the brain highlighting its significance in neural processes.
Biological function summary

The TRIM9 protein regulates axon pathfinding and synapse formation influencing neural network development. TRIM9 forms complexes with other proteins to modulate cytoskeletal dynamics affecting neuron shape and connectivity. By interacting with proteins like DCC (Deleted in Colorectal Carcinoma) TRIM9 plays a role in guiding axons during neural circuit formation. This interaction is essential for creating proper neuronal connections and ensuring functional communication within the brain.

Pathways

TRIM9 emerges as an important regulator in the Netrin-1 signaling pathway critical for axonal guidance. This pathway involves interaction with DCC a receptor for Netrin-1 influencing axonal steering decisions. Additionally TRIM9 participates in the ubiquitination pathway affecting protein stability and cellular localization that modulates various signaling cascades within neurons. By manipulating these pathways TRIM9 assists in neural development and plasticity.

Alterations in TRIM9 expression or function correlate with neurological conditions such as schizophrenia and autism spectrum disorders. Dysfunctional TRIM9-mediated pathways might disrupt neural circuit development contributing to the cognitive and social impairments associated with these disorders. Other proteins like DCC also share connections with these disorders indicating a network of interactions where TRIM9 influences disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which ubiquitinates itself in cooperation with an E2 enzyme UBE2D2/UBC4 and serves as a targeting signal for proteasomal degradation. May play a role in regulation of neuronal functions and may also participate in the formation or breakdown of abnormal inclusions in neurodegenerative disorders. May act as a regulator of synaptic vesicle exocytosis by controlling the availability of SNAP25 for the SNARE complex formation.
See full target information TRIM9
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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